Serial Analysis of Gene Expression Adapted for Downsized Extracts (SAGE/SADE) Analysis in Reticulocytes

International audienc

Open Data on ANR-funded research: a valuable resource to explore impact pathways in research funding

ANR is the French national research agency founded in 2005 and responsible for the project-based research funding. As part of ANR’s “open science” initiative, the Agency ensures the circulation of knowledge on public grants by providing data for each funded project in standardized formats to facilitate their broad and easy use. These data provide information on the research funded by ANR since 2005 across all scientific fields. In the present poster, we will first describe the type of data made available to the public which provides detailed information on the projects grants funded by ANR from 2005 to 2017. Using three proof-of-principle examples, we also illustrate how this data could be exploited and investigated, at different levels (institutions, PIs and research themes) to study the impacts of ANR on the national scientific production. In all three examples, results obtained from the ANR database were confronted to similar metrics obtained from external sources, in particular the Web of Science (WoS). Specifically, we identified a main pool of publications by querying the WoS database for all publications in which ANR was acknowledged (closed to 100,000 publications between 2006 and 2018). These examples illustrate how the ANR dataset could be completed by additional resources (e.g. bibliographic databases, European funding, patents data, open archives, ORCID, altmetrics data) to yield interesting results describing research funding and production. As such, dissemination of these data, combined with other research data, and extension, refinements of these analyses should contribute to decipher and enlighten the trends of research, the dynamics of scientific networks in France, in Europe and worldwide

Open Data on ANR-funded research: a valuable resource to explore impact pathways in research funding

ANR is the French national research agency founded in 2005 and responsible for the project-based research funding. As part of ANR’s “open science” initiative, the Agency ensures the circulation of knowledge on public grants by providing data for each funded project in standardized formats to facilitate their broad and easy use. These data provide information on the research funded by ANR since 2005 across all scientific fields. In the present poster, we will first describe the type of data made available to the public which provides detailed information on the projects grants funded by ANR from 2005 to 2017. Using three proof-of-principle examples, we also illustrate how this data could be exploited and investigated, at different levels (institutions, PIs and research themes) to study the impacts of ANR on the national scientific production. In all three examples, results obtained from the ANR database were confronted to similar metrics obtained from external sources, in particular the Web of Science (WoS). Specifically, we identified a main pool of publications by querying the WoS database for all publications in which ANR was acknowledged (closed to 100,000 publications between 2006 and 2018). These examples illustrate how the ANR dataset could be completed by additional resources (e.g. bibliographic databases, European funding, patents data, open archives, ORCID, altmetrics data) to yield interesting results describing research funding and production. As such, dissemination of these data, combined with other research data, and extension, refinements of these analyses should contribute to decipher and enlighten the trends of research, the dynamics of scientific networks in France, in Europe and worldwide

Rat G protein-coupled recptor kinase GRK4 : identification, functional expression, and differential tissue distribution of two splice variants

International audienc

Rat G protein-coupled recptor kinase GRK4 : identification, functional expression, and differential tissue distribution of two splice variants

International audienc

G Protein-coupled Receptor Kinase 4 (GRK4) Regulates the Phosphorylation and Function of the Dopamine D3 Receptor*

During conditions of moderate sodium excess, the dopaminergic system regulates blood pressure and water and electrolyte balance by engendering natriuresis. Dopamine exerts its effects on dopamine receptors, including the dopamine D3 receptor. G protein-coupled receptor kinase 4 (GRK4), whose gene locus (4p16.3) is linked to essential hypertension, desensitizes the D1 receptor, another dopamine receptor. This study evaluated the role of GRK4 on D3 receptor function in human proximal tubule cells. D3 receptor co-segregated in lipid rafts and co-immunoprecipitated and co-localized in human proximal tubule cells and in proximal and distal tubules and glomeruli of kidneys of Wistar Kyoto rats. Bimolecular fluorescence complementation and confocal microscopy revealed that agonist activation of the receptor initiated the interaction between D3 receptor and GRK4 at the cell membrane and promoted it intracellularly, presumably en route to endosomal trafficking. Of the four GRK4 splice variants, GRK4-γ and GRK4-α mediated a 3- and 2-fold increase in the phosphorylation of agonist-activated D3 receptor, respectively. Inhibition of GRK activity with heparin or knockdown of GRK4 expression via RNA interference completely abolished p44/42 phosphorylation and mitogenesis induced by D3 receptor stimulation. These data demonstrate that GRK4, specifically the GRK4-γ and GRK4-α isoforms, phosphorylates the D3 receptor and is crucial for its signaling in human proximal tubule cells