Direct Nitrous Oxide Emissions From Tropical And Sub-Tropical Agricultural Systems : A Review and Modelling of Emission Factors

We acknowledge the financial support from the CGIAR Research Programs on Climate Change, Agriculture and Food Security (CCAFS). Grant ref. n. P25.Peer reviewedPublisher PD

Identification of low-frequency TRAF3IP2 coding variants in psoriatic arthritis patients and functional characterization

Introduction: In recent genome-wide association studies for psoriatic arthritis (PsA) and psoriasis vulgaris, common coding variants in the TRAF3IP2 gene were identified to contribute to susceptibility to both disease entities. The risk allele of p.Asp10Asn (rs33980500) proved to be most significantly associated and to encode a mutant protein with an almost completely disrupted binding property to TRAF6, supporting its impact as a main disease-causing variant and modulator of IL-17 signaling. Methods: To identify further variants, exons 2-4 encoding both known TNF-receptor-associated factor (TRAF) binding domains were sequenced in 871 PsA patients. Seven missense variants and one three-base-pair insertion were identified in 0.06% to 1.02% of alleles. Five of these variants were also present in 931 control individuals at comparable frequency. Constructs containing full-length wild-type or mutant TRAF3IP2 were generated and used to analyze functionally all variants for TRAF6-binding in a mammalian two-hybrid assay. Results: None of the newly found alleles, though, encoded proteins with different binding properties to TRAF6, or to the cytoplasmic tail of the IL-17-receptor α-chain, suggesting that they do not contribute to susceptibility. Conclusions: Thus, the TRAF3IP2-variant p.Asp10Asn is the only susceptibility allele with functional impact on TRAF6 binding, at least in the German population

Late Holocene forcing of the Asian winter and summer monsoon as evidenced by proxy records from the northern Qinghai-Tibetan Plateau

Little is known about decadal- to centennial-scale climate variability and its associated forcing mechanisms on the Qinghai-Tibetan Plateau. A decadal-resolution record of total organic carbon (TOC) and grainsize retrieved from a composite piston core from Kusai Lake, NW China, provides solid evidence for decadal- to centennial-scale Asian monsoon variability for the Northern Qinghai-Tibetan Plateau during the last 3770 yr. Intensified winter and summer monsoons are well correlated with respective reductions and increases in solar irradiance. A number of intensified Asian winter monsoon phases are potentially correlated with North Atlantic climatic variations including Bond events 0 to 2 and more recent subtle climate changes from the Medieval Warm Period to the Little Ice Age. Our findings indicate that Asian monsoon changes during the late Holocene are forced by changes in both solar output and oceanic-atmospheric circulation patterns. Our results demonstrate that these forcing mechanisms operate not only in low latitudes but also in mid-latitude regions (the Northern Qinghai-Tibetan Plateau)

Guiding principles for good governance of the smart destination

Smart tourism development has received tremendous attention worldwide, but relevant literature remains largely focused on the technological aspects of achieving the greater economic growth and efficiency through innovation, mobility, inclusion and environmental sustainability the smart paradigm promises. While well-being and experience enhancement are considered at the individual level of tourists and (sometimes) residents, guiding principles for achieving responsible smart tourism at the destination level are lacking. Addressing these concerns, this paper begins to explore justice and ethics principles that offer the potential to help facilitate good governance of sustainable smart destinations. By merging smart tourism goals with justice and ethics considerations the paper presents preliminary guidelines for theory building, public policy and development practice in the context of smart destinations governance, and future research in this area. Keywords: smart tourism; sustainable development; governance, ethics, justice, responsible touris

Ureteroscopic and Scanning Electron Microscopical Findings in Ureteral Anastomoses After Segment Resection

In an experimental study on 8 canine ureters, end-to-end anastomoses were performed after segmental resection between 9 and 13 cm, using two different surgical techniques. After 3 months of observation the three-dimensional structure of the anastomosis and the surrounding areas was investigated by ureteroscopy and scanning electron microscopy. 3 types could be differentiated by the morphologic features of the ureteral lumen: normal width, segmental stricture, and circumferential stricture. The results offer a plausible explanation for the occasional discrepancy between histologic findings of relatively normal lumen width, and functional disorders apparently reflecting ureteral stenosis. They further support that excellent functional and morphologic reconstruction can be achieved even under the extreme conditions of 13 cm segmental resection with obligatory anastomosis under tension. The findings connected with dilatations observed distal to the anastomotic site appear rather contradictory, their interpretation is difficult

The 2HA line of Medicago truncatula has characteristics of an epigenetic mutant that is weakly ethylene insensitive

BACKGROUND The Medicago truncatula 2HA seed line is highly embryogenic while the parental line Jemalong rarely produces embryos. The 2HA line was developed from one of the rare Jemalong regenerates and this method for obtaining a highly regenerable genotype in M. truncatula is readily reproducible suggesting an epigenetic mechanism. Microarray transcriptomic analysis showed down regulation of an ETHYLENE INSENSITIVE 3-like gene in 2HA callus which provided an approach to investigating epigenetic regulation of genes related to ethylene signalling and the 2HA phenotype. Ethylene is involved in many developmental processes including somatic embryogenesis (SE) and is associated with stress responses. RESULTS Microarray transcriptomic analysis showed a significant number of up-regulated transcripts in 2HA tissue culture, including nodule and embryo specific genes and transposon-like genes, while only a few genes were down-regulated, including an EIN3-like gene we called MtEIL1. This reduced expression was associated with ethylene insensitivity of 2HA plants that was further investigated. The weak ethylene insensitivity affected root and nodule development. Sequencing of MtEIL1 found no difference between 2HA and wild-type plants. DNA methylation analysis of MtEIL1 revealed significant difference between 2HA and wild-type plants. Tiling arrays demonstrated an elevated level of miRNA in 2HA plants that hybridised to the antisense strand of the MtEIL1 gene. AFLP-like methylation profiling revealed more differences in DNA methylation between 2HA and wild-type. Segregation analysis demonstrated the recessive nature of the eil1 phenotype and the dominant nature of the SE trait. CONCLUSIONS We have demonstrated that EIL1 of Medicago truncatula (MtEIL1) is epigenetically silenced in the 2HA seed line. The possible cause is an elevated level of miRNA that targets its 3'UTR and is also associated with DNA methylation of MtEIL1. Down regulation of MtEIL1 makes it possible to form nodules in the presence of ethylene and affects root growth under normal conditions. Segregation analysis showed no association between MtEIL1 expression and SE in culture but the role and mechanism of ethylene signalling in the process of plant regeneration through SE requires further investigation. The work also suggests that epigenetic changes to a particular gene induced in culture can be fixed in regenerated plants.This work was funded by the Australian Research Council (CEO348212) through the ARC Centre of Excellence for Integrative Legume Research (CILR)

Detection of arabis mosaic virus using the polymerase chain reaction (PCR)

A new method is described for detecting arabis mosaic virus (ArMV) in infected plants. Specific sequences of ArMV-RNA present in total nucleic acid extracts of infected Vitis vinifera or Chenopodium quinoa were initially reverse-transcribed into a complementary DNA (cDNA), then amplified by PCR using specific oligonucleotide-primers. Different primer combinations distinguished between an ArMV infection and an infection with grapevine fanleaf or raspberry ringspot virus. The amount of nucleic acids obtained from 5 mg grapevine leaves resp. 1 mg leaves of Ch. quinoa were sufficient for detecting ArMV