49 research outputs found

    Insulin Increases Ceramide Synthesis in Skeletal Muscle

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    Aims. The purpose of this study was to determine the effect of insulin on ceramide metabolism in skeletal muscle. Methods. Skeletal muscle cells were treated with insulin with or without palmitate for various time periods. Lipids (ceramides and TAG) were isolated and gene expression of multiple biosynthetic enzymes were quantified. Additionally, adult male mice received daily insulin injections for 14 days, followed by muscle ceramide analysis. Results. In muscle cells, insulin elicited an increase in ceramides comparable to palmitate alone. This is likely partly due to an insulin-induced increase in expression of multiple enzymes, particularly SPT2, which, when knocked down, prevented the increase in ceramides. In mice, 14 days of insulin injection resulted in increased soleus ceramides, but not TAG. However, insulin injections did significantly increase hepatic TAG compared with vehicle-injected animals. Conclusions. This study suggests that insulin elicits an anabolic effect on sphingolipid metabolism in skeletal muscle, resulting in increased ceramide accumulation. These findings reveal a potential mechanism of the deleterious consequences of the hyperinsulinemia that accompanies insulin resistance and suggest a possible novel therapeutic target to mitigate its effects

    Development of an Electrofluidic Rate Sensor

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    Assessment of impoundment and forfeiture laws for drivers convicted of DUI. Phase II report: evaluation of Oregon and Washington Vehicle Plate Zebra Sticker Laws. Final report.

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    National Highway Traffic Safety Administration, Washington, D.C.Mode of access: Internet.Author corporate affiliation: National Public Services Research Institute, Landover, Md.Report covers the period 12/89 - 4/94Subject code: DYISubject code: JLKSubject code: RCCDDSubject code: RPSubject code: RRCSubject code: WW
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