PROTEOMIC ANALYSIS OF HUMAN BLOOD AND URINE IN DIABETIC NEPHROPATHY

Objective: To investigate the proteomics status of human blood and urine in diabetic nephropathy.Methods: In the present study 90 patients were selected. The study was comprised of 30 Diabetic mellitus (DM) with microalbuminuria patients (Group 3), 30 DM without microalbuminuria patients (group 2), 30 healthy controls (Group 1). Fasting glucose, post prandial glucose, lipid profile, fructosamine in serum and micro albumin in urine were investigated in all the patients.Results: The significant increase in serum fructosamine, fasting and post prandial glucose levels along with increased microalbuminuria observed in group 3 patients compared to group 2 and group 1 patients. Hyperglycemia increases fructosamine, cholesterol, triglycerides with decrease in HDL-cholesterol levels, indicates the major risk of atherogenicity. To study the effect of age, smoking, DM duration on DN, patients were grouped in different way and analysed.Conclusion: The results suggested that smoking, age and prolonged DM influences DN. Normal and DN patients serum and urine samples were selected and protein was separated by SDS-PAGE and identified by LC-MS. Results of LC-MS showed the difference in proteomics of normal and DN patients

Tropical Dominating Sets in Vertex-Coloured Graphs

Given a vertex-coloured graph, a dominating set is said to be tropical if every colour of the graph appears at least once in the set. Here, we study minimum tropical dominating sets from structural and algorithmic points of view. First, we prove that the tropical dominating set problem is NP-complete even when restricted to a simple path. Then, we establish upper bounds related to various parameters of the graph such as minimum degree and number of edges. We also give upper bounds for random graphs. Last, we give approximability and inapproximability results for general and restricted classes of graphs, and establish a FPT algorithm for interval graphs.Comment: 19 pages, 4 figure

Targeting Glycosylation Pathways and the Cell Cycle: Sugar-Dependent Activity of Butyrate-Carbohydrate Cancer Prodrugs

SummaryShort-chain fatty acid (SCFA)-carbohydrate hybrid molecules that target both histone deacetylation and glycosylation pathways to achieve sugar-dependent activity against cancer cells are described in this article. Specifically, n-butyrate esters of N-acetyl-d-mannosamine (But4ManNAc, 1) induced apoptosis, whereas corresponding N-acetyl-d-glucosamine (But4GlcNAc, 2), d-mannose (But5Man, 3), or glycerol (tributryin, 4) derivatives only provided transient cell cycle arrest. Western blots, reporter gene assays, and cell cycle analysis established that n-butyrate, when delivered to cells via any carbohydrate scaffold, functioned as a histone deacetylase inhibitor (HDACi), upregulated p21WAF1/Cip1 expression, and inhibited proliferation. However, only 1, a compound that primed sialic acid biosynthesis and modulated the expression of a different set of genes compared to 3, ultimately killed the cells. These results demonstrate that the biological activity of butyrate can be tuned by sugars to improve its anticancer properties

Monoclonal antibody-conjugated dendritic nanostructures for siRNA delivery

Small interfering RNA (siRNA) is a promising tool for gene therapy-based disease treatments. However, delivery of siRNA to the target cells requires a specific and reliable carrier system. Herein we describe a targeted carrier system that can deliver siRNA to cancer cells overexpressing the human epidermal growth factor 2 (HER2) receptor. Trastuzumab-conjugated poly(amido)amine dendrimers can be synthesized using the protocols described here

Demonstration of Mussel Farming in Karnataka: A Success Story

Spat settlement along the Karnataka coast occurs during October to November. The mussel fishing season in the region also begins from October. The dense attachment of mussel spats occurs along with adult mussels in the intertidal and subtidal areas, which can be productively utilized for farming, provided a considerable numbers of farmers are involved to establish and maintain the link between mussel farmers and mussel fishers

Vertebral osteomyelitis and native valve endocarditis due to Staphylococcus simulans: a case report

This is an Open Access article distributed under the terms of the Creative Commons Attribution Licens

CMV infection of liver transplant recipients: comparison of antigenemia and molecular biology assays

BACKGROUND: CMV is a major clinical problem in transplant recipients. Thus, it is important to use sensitive and specific diagnostic techniques to rapidly and accurately detect CMV infection and identify patients at risk of developing CMV disease. In the present study, CMV infection after liver transplantation was monitored retrospectively by two molecular biology assays - a quantitative PCR assay and a qualitative NASBA assay. The results were compared with those obtained by prospective pp65 antigenemia determinations. MATERIALS AND METHODS: 87 consecutive samples from 10 liver transplanted patients were tested for CMV by pp65 antigenemia, and CMV monitor and NASBA pp67 mRNA assay. RESULTS: CMV infection was detected in all patients by antigenemia and CMV monitor, whereas NASBA assay identified only 8/10 patients with viremia. Furthermore, CMV infection was never detected earlier by molecular biology assays than by antigenemia. Only 5/10 patients with CMV infection developed CMV disease. Using a cut off value of 8 cells/50,000, antigenemia was found to be the assay that better identified patients at risk of developing CMV disease. However, the kinetics of the onset of infection detected by NASBA and CMV monitor seemed to have better identified patients at risk of developing CMV disease. Furthermore, before onset of disease, CMV pp67 mRNA was found to have similar or better negative and positive predictive values for the development of CMV disease. CONCLUSIONS: The present data, suggests that the concomitant use of antigenemia and pp67 mRNA assay gives the best identification of patients at risk of developing CMV disease