584 research outputs found
Molecular profiling of resident and infiltrating mononuclear phagocytes during rapid adult retinal degeneration using single-cell RNA sequencing.
Neuroinflammation commonly accompanies neurodegeneration, but the specific roles of resident and infiltrating immune cells during degeneration remains controversial. Much of the difficulty in assessing myeloid cell-specific functions during disease progression arises from the inability to clearly distinguish between activated microglia and bone marrow-derived monocytes and macrophages in various stages of differentiation and activation within the central nervous system. Using an inducible model of photoreceptor cell death, we investigated the prevalence of infiltrating monocytes and macrophage subpopulations after the initiation of degeneration in the mouse retina. In vivo retinal imaging revealed infiltration of CCR2+ leukocytes across retinal vessels and into the parenchyma within 48 hours of photoreceptor degeneration. Immunohistochemistry and flow cytometry confirmed and characterized these leukocytes as CD11b+CD45+ cells. Single-cell mRNA sequencing of the entire CD11b+CD45+ population revealed the presence of resting microglia, activated microglia, monocytes, and macrophages as well as 12 distinct subpopulations within these four major cell classes. Our results demonstrate a previously immeasurable degree of molecular heterogeneity in the innate immune response to cell-autonomous degeneration within the central nervous system and highlight the necessity of unbiased high-throughput and high-dimensional molecular techniques like scRNAseq to understand the complex and changing landscape of immune responders during disease progression
Determining the underlying Fermi surface of strongly correlated superconductors
The notion of a Fermi surface (FS) is one of the most ingenious concepts
developed by solid state physicists during the past century. It plays a central
role in our understanding of interacting electron systems. Extraordinary
efforts have been undertaken, both by experiment and by theory, to reveal the
FS of the high temperature superconductors (HTSC), the most prominent strongly
correlated superconductors. Here, we discuss some of the prevalent methods used
to determine the FS and show that they lead generally to erroneous results
close to half filling and at low temperatures, due to the large superconducting
gap (pseudogap) below (above) the superconducting transition temperature. Our
findings provide a perspective on the interplay between strong correlations and
superconductivity and highlight the importance of strong coupling theories for
the characterization as well as the determination of the underlying FS in ARPES
experiments
Magnetocrystalline anisotropic effect in GdCoFeAsO ()
From a systematic study of the electrical resistivity , magnetic
susceptibility , isothermal magnetization and the specific
heat , a temperature-magnetic field (-) phase diagram has been
established for GdCoFeAsO ( and ) polycrystalline
compounds. GdCoAsO undergoes two long-range magnetic transitions: ferromagnetic
(FM) transition of Co electrons () and
antiferromagnetic (AFM) transition of Gd electrons
(). For the Fe-doped sample (), an extra
magnetic reorientation transition takes place below ,
which is likely associated with Co moments. The two magnetic species of Gd and
Co are coupled antiferromagnetically to give rise to ferrimagnetic (FIM)
behavior in the magnetic susceptibility. Upon decreasing the temperature (), the magnetocrystalline anisotropy breaks up the FM
order of Co by aligning the moments with the local easy axes of the various
grains, leading to a spin reorientation transition at
. By applying a magnetic field,
monotonically decreases to lower temperatures, while
the is relatively robust against the external field.
On the other hand, the applied magnetic field pulls the magnetization of grains
from the local easy direction to the field direction via a first-order
reorientation transition, with the transition field () increasing
upon cooling the temperature.Comment: accepted by physical Review B 6 figures and 7 page
Superconductivity in SrNi2As2 Single Crystals
The electrical resistivity \rho(T) and heat capacity C(T) on single crystals
of SrNi2As2 and EuNi2As2 are reported. While there is no evidence for a
structural transition in either compound, SrNi2As2 is found to be a bulk
superconductor at T_c=0.62 K with a Sommerfeld coefficient of \gamma= 8.7
mJ/mol K^2 and a small upper critical field H_{c2} \sim 200 Oe. No
superconductivity was found in EuNi2As2 above 0.4 K, but anomalies in \rho and
C reveal that magnetic order associated with the Eu^{2+} magnetic moments
occurs at T_m = 14 K.Comment: 8 pages, 5 figure
Effect of annealing on the specific heat of Ba(Fe1-xCox)2As2
We report on the effect of annealing on the temperature and field
dependencies of the low temperature specific heat of the electron-doped
Ba(FeCo)As for under-(x = 0.045), optimal- (x = 0.08)
and over-doped (x = 0.105 and 0.14) regimes. We observed that annealing
significantly improves some superconducting characteristics in
Ba(FeCo)As. It considerably increases ,
decreases in the superconducting state and suppresses the
Schottky-like contribution at very low temperatures. The improved sample
quality allows for a better identification of the superconducting gap structure
of these materials. We examine the effects of doping and annealing within a
self-consistent framework for an extended s-wave pairing scenario. At optimal
doping our data indicates the sample is fully gapped, while for both under and
overdoped samples significant low-energy excitations possibly consistent with a
nodal structure remain. The difference of sample quality offers a natural
explanation for the variation in low temperature power laws observed by many
techniques.Comment: 9 pages: added references, two figures and supplementary information;
Accepted to Physical Review B (Jan 10, 2010
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