EFFLUX MEDIATED MULTIDRUG RESISTANT PSEUDOMONAS AERUGINOSA ISOLATED FROM DIFFERENT ENVIRONMENTAL SOURCES

Pseudomonas aeruginosa is an important opportunistic pathogen and one of the leading causes of multi-drug resistant nosocomial infections. This study was therefore carried out to determine the resistance nature, and the role of efflux pump in multidrug resistance of Pseudomonas aeruginosa isolated from different environmental sources using the efflux pump inhibitor, Carbonyl Cyanide 3-Chlorophenylhydrazone (CCCP). A total of 220 environmental samples were collected and processed following standard techniques. Susceptibility to antibiotics was performed using disc diffusion methods as described by the Clinical and Laboratory Standards Institute. Activity of the efflux pump system was carried out using the efflux pump inhibitor, CCCP. Results obtained identified 100 (45.5%) Pseudomonas aeruginosa and 72 (32.7%) other strains of Pseudomonas spp. The susceptibility testing revealed that all the identified strains of Pseudomonas aeruginosa that were subjected to susceptibility test were significantly resistant to ampicillin and cefotaxime, But the  resistance profile of isolates to tetracycline, chloramphenicol, ceftriaxone, cefuroxime and perfloxacin were 93%, 72.1%, 79.1%, 58.1% and 51.2% respectively. However, imipenem was the most sensitive (100%), followed by cefepime (65%) and gentamicin (44%). Carbonyl Cyanide 3-Chlorophenylhydrazone decreased the minimum inhibitory concentration (MIC) of the isolates by 2 folds. Results obtained have shown the ubiquitous presence of multi-drug resistant P. aeruginosa from the environmental samples examined. Furthermore, it indicated the role of efflux pump in antibiotics resistance in P. aeruginosa isolates which indicate that P. aeruginosa strains from environmental sources could resist antibiotics by the efflux mechanism.     &nbsp

AN EVALUATION OF THE ENTREPRENEURIAL INCLINATION AMONG CLOTHING AND TEXTILE UNDERGRADUATE IN SOUTH WESTERN NIGERIA

Entrepreneurship has been described as an incubator and energiser of market innovation, product and technological growth as well as a means of revitalising the economy and reducing unemployment. This study focused on assessing the entrepreneurship inclination among Clothing and Textile undergraduates and their attitude towards government entrepreneurial programmes. The study was carried out in selected tertiary institutions (Colleges of Education, Polytechnics and Universities) in South-Western Nigeria. A Multistage sampling was used with a combination of stratified and proportionate sampling to select four hundred and fifty (450) final year students from the selected tertiary institutions in South-Western Nigeria. Data were collected with the aid of questionnaire and were analysed using descriptive and inferential statistical tools. Results showed that most of the respondents were female (55.3%) while (44.7%) were male with a mean age of 22 years. More than half (54%) of the respondents were from Colleges of Education, 23.7% were from Universities and 22.2% were from Polytechnics. The findings further revealed that 30.4%, 24%, 19.6% and 14.2% were studying Home Economics, Creative arts, Fine arts and Clothing and Textiles respectively. The percentage of response on the students’ attitude towards entrepreneurship was 87.3%. The results of the chi-square showed that only the educational status of the respondents (χ2=14.65, p<0.05) indicated a significant relationship between the educational status of the students and their entrepreneurial inclination. The regression analysis showed that there was a significant correlation between government empowerment programme and the entrepreneurial inclination of the respondents (r = 0.43, P = 0.00). The β value of 0.43 explained the percentage of government empowerment programme to entrepreneurial inclination of the respondents. Also, there was no significant difference between entrepreneurial skills inclination of undergraduate students across the selected tertiary institutions at P < 0.05 level of significance. The study concluded that respondents in the study area have positive attitude towards government entrepreneurship programme. Consequently, the respondents are entrepreneurial inclined and government entrepreneurship programme is effective to reduce unemployment in the country and in promoting job creation. &nbsp

Effects of cadmium perturbation on the microbial community structure and heavy metal resistome of a tropical agricultural soil

The effects of cadmium (Cd) contamination on the microbial community structure, soil physicochemistry and heavy metal resistome of a tropical agricultural soil were evaluated in field-moist soil microcosms. A Cd-contaminated agricultural soil (SL5) and an untreated control (SL4) were compared over a period of 5 weeks. Analysis of the physicochemical properties and heavy metals content of the two microcosms revealed a statistically significant decrease in value of the soil physicochemical parameters (P < 0.05) and concentration of heavy metals (Cd, Pb, Cr, Zn, Fe, Cu, Se) content of the agricultural soil in SL5 microcosm. Illumina shotgun sequencing of the DNA extracted from the two microcosms showed the predominance of the phyla, classes, genera and species of Proteobacteria (37.38%), Actinobacteria (35.02%), Prevotella (6.93%), and Conexibacter woesei (8.93%) in SL4, and Proteobacteria (50.50%), Alphaproteobacteria (22.28%), Methylobacterium (9.14%), and Methylobacterium radiotolerans (12,80%) in SL5, respectively. Statistically significant (P < 0.05) difference between the metagenomes was observed at genus and species delineations. Functional annotation of the two metagenomes revealed diverse heavy metal resistome for the uptake, transport, efflux and detoxification of various heavy metals. It also revealed the exclusive detection in SL5 metagenome of members of RND (resistance nodulation division) protein czcCBA efflux system (czcA, czrA, czrB), CDF (cation diffusion facilitator) transporters (czcD), and genes for enzymes that protect the microbial cells against cadmium stress (sodA, sodB, ahpC). The results obtained in this study showed that Cd contamination significantly affects the soil microbial community structure and function, modifies the heavy metal resistome, alters the soil physicochemistry and results in massive loss of some autochthonous members of the community not adapted to the Cd stress

Anthropometric design of furniture for use in tertiary institutions in Abeokuta, South-western Nigeria

Students sit for most of the time on school furniturein the classroom. However, the design of schoolfurniture in Nigeria using the anthropometric dataof the users has yet to be given adequate attention.This study was carried out to obtainanthropometric data of students in higherinstitutions of learning in Nigeria for the design ofergonomics chairs and tables for use by students inthose institutions. Seven hundred and twenty (720)students in three selected institutions participatedin the study. Various body dimensions (sittingelbow height, shoulder height, knee height,popliteal height, buttock-popliteal length, statureand body weight) of the students were measuredusing standard anthropometer and 5 th, 50th, and95 th percentiles of the data obtained werecomputed using a SPSS 16.0 statistical package.The existing furniture dimensions in the selectedinstitutions were also measured. Based on theobtained anthropometric data, this paper proposesfurniture design dimensions for seat height, seatdepth, seat width, backrest height (upper), armrestand desk height for students in the selected tertiaryinstitutions. The present study may be a pointer tothe effect that in the design of tables and chairs foruse in higher institutions, the anthropometric dataof the Nigerian students were not considered. It ishereby recommended that similar study should becarried out in other sections of the country

COMPARATIVE ASSESSMENT OF CARBON STORAGE IN BIOMASS AND SOIL ORGANIC CARBON IN TEAK PLANTATION OF DIFFERENT AGES IN YEWA NORTH, OGUN STATE, NIGERIA

Tree act as a sink for CO2 by fixing carbon during photosynthesis and is a vital tool to alleviate climate change through CO2 absorption from the atmosphere. This study was conducted to assess carbon storage in tree biomass and soil carbon stock in Teak (Tectona grandis) plantations of different age series (5, 9 and 12 years). Data were collected on diameter at breast height (Dbh) and total height (TH) of all the trees in a sample plot of 1000 m2 in each plantation of age series. Carbon stored was evaluated based on tree growth variables, soil parameters and above ground biomass. Topsoil and subsoil (0-15 and 15-30 cm) samples collected from three sites were analyzed for organic carbon (OC) following standard methods.&nbsp; Mean diameter at breast height were 67.11, 45.42, and 21.35 cm ha-1 and the mean volumes were 0.39, 0.16 and 0.02 m3ha-1 for 12, 9 and 5 years old were significant different (p&lt;0.05). The highest value of total soil organic carbon (TSOC) was 8464.65 t.ha-1 for the 12year-old followed by 4430.25 t.ha-1 for 9 years old stand and 3004.95 t.ha-1 for 5years old were significant different (p&lt;0.05) respectively. The total soil organic carbon per hectare were higher for the older than the younger Tectona grandis stand (8464.65 t.ha-1, 4430.25 t.ha-1 and 3004.95 t.ha-1) were significant different (p&lt;0.05). Total carbon storage by soil and in biomass (CSB) was 15899.85 and 49.31 tons.ha-1 and total carbon accumulated by the plantation under investigation was in the order of 12 years old &gt; 9 years old &gt; 5 years old, respectively. The results of this study confirm that teak has good potentials to offer carbon sequestration through its soil and accumulate large amount of biomass carbon. The plantation ownership should be guided properly on the management activities such as thinning, pruning and weeding operation as well as indiscriminate removal of individual caused by anthropogenic activities should be avoided. &nbsp; &nbsp; &nbsp

In silico identification of the potential natural inhibitors of SARS-CoV-2 Guanine-N7 methyltransferase

This study aims at computationally identifying the potential natural inhibitors of the SARS-CoV-2 Guanine-N7 methyltransferase binding at the active sit

Global analysis of data on the spin-orbit coupled A1Σu+A^{1}\Sigma_{u}^{+} and b3Πub^{3}\Pi_{u} states of Cs2

We present experimentally derived potential curves and spin-orbit interaction functions for the strongly perturbed $A^{1}\Sigma_{u}^{+}$ and $b^{3}\Pi_{u}$ states of the cesium dimer. The results are based on data from several sources. Laser-induced fluorescence Fourier transform spectroscopy (LIF FTS) was used some time ago in the Laboratoire Aim\'{e} Cotton primarily to study the $X ^{1}\Sigma_{g}^{+}$ state. More recent work at Tsinghua University provides information from moderate resolution spectroscopy on the lowest levels of the $b^{3}\Pi_{0u}^{\pm}$ states as well as additional high resolution data. From Innsbruck University, we have precision data obtained with cold Cs$_{2}$ molecules. Recent data from Temple University was obtained using the optical-optical double resonance polarization spectroscopy technique, and finally, a group at the University of Latvia has added additional LIF FTS data. In the Hamiltonian matrix, we have used analytic potentials (the Expanded Morse Oscillator form) with both finite-difference (FD) coupled-channels and discrete variable representation (DVR) calculations of the term values. Fitted diagonal and off-diagonal spin-orbit functions are obtained and compared with {\it ab initio} results from Temple and Moscow State universities