Partycypacja społeczna w kształtowaniu przestrzeni centralnej wsi jerzmanowice usytułowanej w otulinie Ojcowskiego Parku Narodowego

Suburban villages undergo dynamic transformations that significantly affect their spatial and social structure. Particular attention should be paid to those locations situated within an attractive landscape, where investment pressure is gaining momentum. The threat of implementing new functions at the expense of open areas increases with an attractive location in the vicinity of large cities. The situation described herein concerns the Jerzmanowice-Przeginia municipality, which is adjoining the Ojców National Park. The activities on the part of municipal authorities, aimed at protecting the identity of the village, are conducted on two planes: related to land planning, and to architecture. Students from the University of Agriculture were involved in the architectural scope of planning, and they developed concepts for the management of the central area of Jerzmanowice village based on the results of social research carried out by the staff of the Faculty of Biotechnology and Horticulture at the University of Agriculture in Kraków.Wsie podmiejskie ulegają dynamicznym przekształceniom, które znacząco wpływają na ich strukturę przestrzenną i społeczną. Szczególną uwagę należy zwrócić na miejscowości usytuowane w atrakcyjnym krajobrazie, gdzie presja inwestycyjna przybiera na sile. Zagrożenie realizacją nowych funkcji kosztem terenów otwartych wzrasta wraz z atrakcyjną lokalizacją w sąsiedztwie dużych miast. Opisana sytuacja dotyczy gminy Jerzmanowice-Przeginia, która sąsiaduje z terenem Ojcowskiego Parku Narodowego. Działania władz gminy, w celu ochrony tożsamości wsi, prowadzone są w dwóch skalach, planistycznej oraz architektonicznej. W działaniach architektonicznych wzięli udział studenci Uniwersytetu Rolniczego, którzy opracowywali koncepcje zagospodarowania przestrzeni centralnej wsi Jerzmanowice na podstawie wyników badań społecznych przeprowadzonych przez pracowników naukowo-dydaktycznych Wydziału Biotechnologii i Ogrodnictwa Uniwersytetu Rolniczego w Krakowie

Preliminary study on the influence of UV-C irradiation on microorganism viability and polyphenol compounds content during winemaking of ‘Regent’ red grape cultivar

In this study, UV-C light was tested as an alternative method to inactivate microorganisms in the must of ‘Regent’ red grape cultivar. The control sample containing the microorganism diluted in a physiological NaCl solution was prepared to take into consideration different conditions of liquids, such as turbidity and colour. Additionally, the changes in the composition of polyphenol compounds in the ‘Regent’ must after UV-C exposure were evaluated. The viability of yeasts (Saccharomyces cerevisiae) and bacteria (Oenococcus oeni) significantly decreased with time; however, the highest decline was noted after the first hour of exposure. The polyphenol compound content was significantly lower after UV-C treatment and this was mainly the result of anthocyanin decomposition. The total content of flavan-3-ols and hydroxycinnamic acids and derivatives increased after irradiation

The determinants and significance of intraluminal stent protrusion in patients with acute myocardial infarction

Abstract Background The mchanisms leading to intraluminal stent protrusion in patients following primary coronary angioplasty for ST-elevation myocardial infarction (STEMI) are not fully elucidated. Purpose We sought to investigate composition of stent protrusion, its systemic and local determinants as well as its consequences for epicardial blood flow restoration in STEMI patients. Methods We enrolled 85 STEMI patients with median age 61 (interquartile range 54–69) years. In all patients optical coherence tomography of an infarct-related artery was performed first following epicardial blood flow restoration after aspiration thrombectomy and second after final stent deployment. On admission, besides standard laboratory investigations we measured calibrated automated thrombogram parameters including time to start clotting, time to peak thrombin generation, peak thrombin generation and endogenous thrombin potential as well as ex vivo measured fibrin clot permeability reflecting fibrin pore size and fibrin clot lysis time. Results A median volume of stent protrusion was 13.4 (8.5–25.4) mm3 while atherothrombotic burden defined as a ratio of free intraluminal thrombus and tissue protrusion divided by stent volume was 6 (3.9–7.5)%. The main component of stent protrusion was lipid tissue in 32 (37.6%), fibrous tissue in 27 (31.8%) and thrombus in 26 (30.6%) patients. A volume of stent protrusion was correlated with total stent volume (R=0.79, P&amp;lt;0.001) and the length of lipid reach pool in the naïve coronary vessel intima (R=0.31, P=0.007) whereas atherothrombotic burden was correlated with residual thrombus volume before stent implantation (R=0.34, P=0.003), arcus of maximal lipid reach pool in the naïve coronary vessel intima (R=0.25, P=0.03) and stent area (R=0.26, P=0.02). The residual thrombus volume after aspiration thrombectomy was correlated with clot permeability (R=0.024, P=0.04) and inversely correlated with time to peak thrombin generation (R=−0.23, P=0.04). A temporary deterioration of epicardial blood flow of at least 1 point in Thrombolysis in Myocardial Infarction scale following stent implantation detected in 20 (23.5%) patients was associated with shorter lag time (3.0 [2.7–3.3] vs. 3.3 [3.0–4.0] min, P=0.015), shorter time to peak thrombin generation (5.7 [5.3–6.1] vs. 6.3 [5.7–7.1] min, P=0.026) and more frequent presence of lipid-rich stent protrusion (65 vs. 29%, P=0.007) as compared to the patients without slow-flow phenomenon after stent deployment. Conclusions The total volume of stent protrusion is correlated with local determinants including stent size and lipid content in the naïve coronary artery intima. After adjustment for stent volume, atherothrombotic burden is additionaly affected by residual thrombus volume after aspiration thrombectomy. However, patients with faster thrombin formation and lipid-rich stent protrusion are more prone to slow-flow phenomenon following stent implantation. Funding Acknowledgement Type of funding sources: Public grant(s) – National budget only. Main funding source(s): National Science Center of Poland </jats:sec

A two-site flexible clamp mechanism for RET-GDNF-GFRα1 assembly reveals both conformational adaptation and strict geometric spacing

AbstractRET receptor tyrosine kinase plays vital developmental and neuroprotective roles in metazoans. GDNF family ligands (GFLs) when bound to cognate GFRα co-receptors recognise and activate RET stimulating its cytoplasmic kinase function. The principles for RET ligand-co-receptor recognition are incompletely understood. Here we report a crystal structure of the cadherin-like module (CLD1-4) from zebrafish RET revealing interdomain flexibility between CLD2-CLD3. Comparison with a cryo-EM structure of a ligand-engaged zebrafish RETECD-GDNF-GFRα1 complex indicates conformational changes within a clade-specific CLD3 loop adjacent to co-receptor. Our observations indicate RET is a molecular clamp with a flexible calcium-dependent arm that adapts to different GFRα co-receptors, while its rigid arm recognises a GFL dimer to align both membrane-proximal cysteine-rich domains. We also visualise linear arrays of RETECD-GDNF-GFRα1 suggesting a conserved contact stabilises higher-order species. Our study reveals ligand-co-receptor recognition by RET involves both receptor plasticity and strict spacing of receptor dimers by GFL ligands.HighlightsCrystal structure of zebrafish RET cadherin-like module reveals conformational flexibility at the calcium-dependent CLD2-CLD3 interfaceComparison of X-ray and cryo-EM structures indicate conformational differences between unliganded and liganded RET involving a clade-specific CLD3 loopStrict spatial separation of RETECD C-termini is imposed by each cysteine-rich domain interaction with GFL dimerDifferences in co-receptor engagement and higher-order ligand-bound RET complexes indicate potentially divergent signalling mechanisms</jats:sec