Bioactivation of 3D Cell-Imprinted Polydimethylsiloxane Surfaces by Bone Protein Nanocoating for Bone Tissue Engineering

Physical and chemical parameters that mimic the physiological niche of the human body have an influence on stem cell fate by creating directional signals to cells. Micro/nano cell-patterned polydimethylsiloxane (PDMS) substrates, due to their ability to mimic the physiological niche, have been widely used in surface modification. Integration of other factors such as the biochemical coating on the surface can achieve more similar microenvironmental conditions and promote stem cell differentiation to the target cell line. Herein, we investigated the effect of physical topography, chemical functionalization by acid bone lysate (ABL) nanocoating, and the combined functionalization of the bone proteins' nanocoated surface and the topographically modified surface. We prepared four distinguishing surfaces: plain PDMS, physically modified PDMS by 3D cell topography patterning, chemically modified PDMS with bone protein nanocoating, and chemically modified nano 3D cell-imprinted PDMS by bone proteins (ABL). Characterization of extracted ABL was carried out by Bradford staining and sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis, followed by the MTT assay for evaluation of cell viability on ABL-coated PDMS. Moreover, field emission scanning electron microscopy and profilometry were used for the determination of optimal coating thickness, and the appropriate coating concentration was identified and used in the study. The binding and retention of ABL to PDMS were confirmed by Fourier transform infrared spectroscopy and bicinchoninic acid assay. Sessile drop static water contact angle measurements on substrates showed that the combined chemical functionalization and nano 3D cell-imprinting on the PDMS surface improved surface wettability by 66% compared to plain PDMS. The results of ALP measurement, alizarin red S staining, immunofluorescence staining, and real-time PCR showed that the nano 3D cell-imprinted PDMS surface functionalized by extracted bone proteins, ABL, is able to guide the fate of adipose derived stem cellss toward osteogenic differentiation. Eventually, chemical modification of the cell-imprinted PDMS substrate by bone protein extraction not only improved the cell adhesion and proliferation but also contributed to the topographical effect itself and caused a significant synergistic influence on the process of osteogenic differentiation

Chromium(III) biosorption onto spent grains residual from brewing industry : equilibrium, kinetics and column studies

The use of industrial wastes for wastewater treatment as a strategy to their re-use and valorisation may provide important advances toward sustainability. The present work gives new insights into heavy metal biosorption onto low-cost biosorbents, studying chromium(III) biosorption onto spent grains residual from a Portuguese brewing industry both in batch and expanded bed column systems. Experimental studies involved unmodified spent grains and spent grains treated with NaOH. Metal uptake followed a rapid initial step, well described by the pseudo-second-order kinetic model up to 27 h, indicating chemisorption to be the rate-limiting step. Beyond this period intraparticle diffusion assumed an important role in the uptake global kinetics. The best fit for equilibrium data was obtained using the Langmuir model, with unmodified spent grains having the higher maximum uptake capacity (q max = 16.7 mg g1). In open system studies, using expanded bed columns, the best performance was also achieved with unmodified spent grains: Breakthrough time (C/C i = 0.25) and total saturation time (C/C i = 0.99) occurred after 58 and 199 h of operation, corresponding to the accumulation of 390 mg of chromium(III), 43.3 % of the total amount entering the column. These results suggest that alkali treatment does not improve spent grains uptake performance. Changes in biomass composition determined by Fourier transform infrared spectroscopy suggested hydroxyl groups and proteins to have an important role in chromium(III) biosorption. This study points out that unmodified spent grains can be successfully used as low-cost biosorbent for trivalent chromium.The authors would like to thank the Portuguese brewing industry UNICER for all the support and FCT (Fundacao para a Ciencia e a Tecnologia) financial support through the Grant PRAXIS XXI/BD/15945/98

The use of a submerged membrane bioreactor for the treatment of a styrene containing synthetic wastewater

In this study, the potential of a membrane bioreactor for treating styrene contaminated synthetic wastewater, with COD that simulated a petrochemical wastewater, was evaluated and compared with previous studies. At hydraulic retention times of 18 and 24 hours, the removal efficiency of COD and styrene was consistently higher than 99%. More significantly no styrene was detected in the exhaust air, which meant that, at both hydraulic retention times, the mechanism of styrene removal in the membrane bioreactor was exclusively through biodegradation. The lower contribution of stripping to overall styrene removal obtained in the present work compared to that previously reported for traditional activated sludge processes was attributed to the higher Mixed Liquor Suspended Solids (MLSS) that can be achieved in a membrane bioreactor. Decrease of hydraulic retention time from 24 h to 18 h also resulted in significant increase in the rate of membrane fouling as quantified by transmembrane pressure gradiant. This was attributed to decrease in extracellular polymeric substance concentration which resulted in sludge deflocculation. As a consequence, the particle size distribution of the activated sludge shifted to lower mean particle sizes and the increase in the percentage of smaller particles resulted in increase in the rate of membrane fouling

Application of homotopy perturbation and numerical methods to the circular porous slider

WOS: 000310411700001Purpose - The purpose of this paper is to present the problem of three-dimensional flow of a fluid of constant density forced through the porous bottom of a circular porous slider moving laterally on a flat plate. Design/methodology/approach - The transformed nonlinear ordinary differential equations are solved via the homotopy perturbation method (HPM) for small as well as moderately large Reynolds numbers. The convergence of the obtained RPM solution is carefully analyzed. Finally, the validity of results is verified by comparing with numerical methods and existing numerical results. Findings - Close agreement of the two sets of results is observed, thus demonstrating the accuracy of the HPM approach for the particular problem considered. Originality/value - Interesting conclusions which can be drawn from this study are that HPM is very effective and simple compared to the existing solution method, able to solve problems without using Pade approximants and can therefore be considered as a clear advantage over the N.M. Bujurke and Phan-Thien techniques

Nanostructured mixed transition metal oxides for high performance asymmetric supercapacitors: facile synthetic strategy

Abstract not availableSanaz Tajik, Deepak P. Dubal, Pedro Gomez-Romero, Amir Yadegari, Alimorad Rashidi, Bahram Nasernejad, Inamuddin, Abdullah M. Asir