Epigenetic analysis of regulatory T cells using multiplex bisulfite sequencing.

This work was supported by Wellcome Trust Grant 096388, JDRF Grant 9-2011-253, the National Institute for Health Research Cambridge Biomedical Research Centre (BRC) and Award P01AI039671 (to LSW. and JAT.) from the National Institute of Allergy and Infectious Diseases (NIAID). CW is supported by the Wellcome Trust (089989). The content of this article is solely the responsibility of the authors and does not necessarily represent the official views of NIAID or the National Institutes of Health. The Cambridge Institute for Medical Research is in receipt of Wellcome Trust Strategic Award 100140. We gratefully acknowledge the participation of all NIHR Cambridge BioResource volunteers. We thank the Cambridge BioResource staff for their help with volunteer recruitment. We thank members of the Cambridge BioResource SAB and Management Committee for their support of our study and the National Institute for Health Research Cambridge Biomedical Research Centre for funding. We thank Fay Rodger and Ruth Littleboy for running the Illumina MiSeq in the Molecular Genetics Laboratories, Addenbrooke's Hospital, Cambridge. This research was supported by the Cambridge NIHR BRC Cell Phenotyping Hub. In particular, we wish to thank Anna Petrunkina Harrison, Simon McCallum, Christopher Bowman, Natalia Savinykh, Esther Perez and Jelena Markovic Djuric for their advice and support in cell sorting. We also thank Helen Stevens, Pamela Clarke, Gillian Coleman, Sarah Dawson, Jennifer Denesha, Simon Duley, Meeta Maisuria-Armer and Trupti Mistry for acquisition and preparation of samples.This is the final version of the article. It first appeared from Wiley via http://dx.doi.org/10.1002/eji.20154564

Epigenetic analysis of regulatory T cells using multiplex bisulfite sequencing

This is the final version of the article. It first appeared from Wiley via http://dx.doi.org/10.1002/eji.201545646This work was supported by Wellcome Trust Grant 096388, JDRF Grant 9-2011-253, the National Institute for Health Research Cambridge Biomedical Research Centre (BRC) and Award P01AI039671 (to LSW. and JAT.) from the National Institute of Allergy and Infectious Diseases (NIAID). CW is supported by the Wellcome Trust (089989). The content of this article is solely the responsibility of the authors and does not necessarily represent the official views of NIAID or the National Institutes of Health. The Cambridge Institute for Medical Research is in receipt of Wellcome Trust Strategic Award 100140. We gratefully acknowledge the participation of all NIHR Cambridge BioResource volunteers. We thank the Cambridge BioResource staff for their help with volunteer recruitment. We thank members of the Cambridge BioResource SAB and Management Committee for their support of our study and the National Institute for Health Research Cambridge Biomedical Research Centre for funding. We thank Fay Rodger and Ruth Littleboy for running the Illumina MiSeq in the Molecular Genetics Laboratories, Addenbrooke's Hospital, Cambridge. This research was supported by the Cambridge NIHR BRC Cell Phenotyping Hub. In particular, we wish to thank Anna Petrunkina Harrison, Simon McCallum, Christopher Bowman, Natalia Savinykh, Esther Perez and Jelena Markovic Djuric for their advice and support in cell sorting. We also thank Helen Stevens, Pamela Clarke, Gillian Coleman, Sarah Dawson, Jennifer Denesha, Simon Duley, Meeta Maisuria-Armer and Trupti Mistry for acquisition and preparation of samples

Linking space and nature syntaxes : the influence of a natural view through observed behaviour at Arcosanti, Arizona, USA

The world’s urban population is rapidly growing, now exceeding its rural population, and is expected to reach 70% of the world’s total by 2050. Research in environmental psychology increasingly supports the Biophilia Hypothesis which holds that our connection with Nature is innate. Thus, how do we maintain a human connection to Nature in an increasingly urbanising world? The research explores the boundary between built and natural environments, specifically how proximity, initially through visual connections, to Nature affects how people use social spaces. Case study work is being undertaken at Arcosanti urban laboratory in the Arizona desert. Through development of a Space/Nature Syntax methodology applied within a uniquely compact urban form, this research attempts to understand how maintaining an instinctive bond with Nature can enhance social interactions and inform future design choices within built environments. Initial results support relationships of varying strengths between spatial connectivity, visibility of Nature, and types of social interactions. This paper explores the potential of the cross-disciplinary Space/Nature Syntax methodology as a design and analysis tool, projecting where social interactions within a built space could be influenced by visibility of Nature; where informed design can allow for the essential human/Nature connection to thrive