Le site Internet Pluies Extrêmes sur la France Métropolitaine

Desplat Julien, Dandin Philippe, Jacq V., Balaguer S., Chapnik B., Croux S., Chassagneux P., Rambaldelli B. Le site Internet Pluies Extrêmes sur la France Métropolitaine. In: Evénements extrêmes fluviaux et maritimes. Leurs variabilités spatiales et chronologiques dans l'ouest de l’Europe. 34èmes journées de l’hydraulique Paris, 1 et 2 février 2012. 2012

Helical Klinotactic Locomotion of Two‐Link Nanoswimmers with Dual‐Function Drug‐Loaded Soft Polysaccharide Hinges

Inspired by the movement of bacteria and other microorganisms, researchers have developed artificial helical micro‐ and nanorobots that can perform corkscrew locomotion or helical path swimming under external energy actuation. In this paper, for the first time the locomotion of nonhelical multifunctional nanorobots that can swim in helical klinotactic trajectories, similarly to rod‐shaped bacteria, under rotating magnetic fields is investigated. These nanorobots consist of a rigid ferromagnetic nickel head connected to a rhodium tail by a flexible hydrogel‐based hollow hinge composed of chemically responsive chitosan and alginate multilayers. This design allows nanoswimmers switching between different dynamic behaviors—from in‐plane tumbling to helical klinotactic swimming—by varying the rotating magnetic field frequency and strength. It also adds a rich spectrum of swimming capabilities that can be adjusted by varying the type of applied magnetic fields and/or frequencies. A theoretical model is developed to analyze the propulsion mechanisms and predict the swimming behavior at distinct rotating magnetic frequencies. The model shows good agreement with the experimental results. Additionally, the biomedical capabilities of the nanoswimmers as drug delivery platforms are demonstrated. Unlike previous designs constitute metallic segments, the proposed nanoswimmers can encapsulate drugs into their hollow hinge and successfully release them to cells.ISSN:2198-384

Improvement of PCR reaction conditions for site-directed mutagenesis of big plasmids

QuickChange mutagenesis is the method of choice for site-directed mutagenesis (SDM) of target sequences in a plasmid. It can be applied successfully to small plasmids (up to 10 kb). However, this method cannot efficiently mutate bigger plasmids. Using KOD Hot Start polymerase in combination with high performance liquid chromatography (HPLC) purified primers, we were able to achieve SDM in big plasmids (up to 16 kb) involving not only a single base change but also multiple base changes. Moreover, only six polymerase chain reaction (PCR) cycles and 0.5 µl of polymerase (instead of 18 PCR cycles and 1.0 µl of enzyme in the standard protocol) were sufficient for the reaction