Locally Derived, Meteoric Fluid Infiltration Was Responsible for Widespread Late Paleozoic Illite Authigenesis in the Appalachian Basin

Isotopic and geochronologic investigation of authigenic, K‐bearing clays in the Appalachian Plateau of the northeastern U.S. Midcontinent yields new insights about the tectonic and diagenetic history of the North American sedimentary cover sequence. In situ texture analysis by High Resolution X‐ray Texture Goniometry indicates preservation of bedding‐parallel diagenetic fabrics with burial depths of 2–5 km, and illite mineralization temperatures are spatially variable, ranging from 80 to 190 °C, correlating to similar depths of 3–6 km. The mineralizing geofluid is surface derived, with δD values ranging from −48‰ to −72‰ (in the range of predicted Pangea meteoric fluid). In addition, we find that mineralizing fluid δD values increase away from the orogenic front, consistent with a rain shadow effect from the high elevation Appalachian orogen. The age of authigenic illite is constrained by 40Ar/39Ar geochronology to 308–318 Ma, reflecting Upper Carboniferous diagenesis. We postulate that far‐field stress transmission from continent‐continent collision created regional permeability pathways for surface fluids, altering the hydrologic architecture of the brittle crust and allowing meteoric fluid infiltration into upper crustal rocks. This interpretation challenges the popular view of tectonically forced, lateral fluid flow from the Appalachian orogen (squeegee hypothesis).Key PointsFar‐field stress due to Alleghanian orogenesis facilitates reorganization of architecture and fluid pathways of the brittle crust and drives regional diagenesisEvidence for locally sourced, surface‐derived fluid challenges popular hypothesis of orogenic fluid expulsionPairing of stable and radiogenic isotope analysis of authigenic illite constrains the relationship between regional diagenesis and tectonismPeer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/156223/2/tect21355.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/156223/1/tect21355_am.pd

Reduced methane emissions from large-scale changes in water management of China’s rice paddies during 1980-2000

Decreased methane emissions from paddy rice may have contributed to the decline in the rate of increase of global atmospheric methane (CH4) concentration over the last 20 years. In China, midseason paddy drainage, which reduces growing season CH4 fluxes, was first implemented in the early 1980s, and has gradually replaced continuous flooding in much of the paddy area. We constructed a regional prediction for China\u27s rice paddy methane emissions using the DNDC biogeochemical model. Results of continuous flooding and midseason drainage simulations for all paddy fields in China were combined with regional scenarios for the timing of the transition from continuous flooding to predominantly mid-season drainage to generate estimates of total methane flux for 1980–2000. CH4 emissions from China\u27s paddy fields were reduced over that period by ∼5 Tg CH4 yr−1

Modeling impacts of farming management alternatives on CO2, CH4, and N2O emissions: A case study for water management of rice agriculture of China

Since the early 1980s, water management of rice paddies in China has changed substantially, with midseason drainage gradually replacing continuous flooding. This has provided an opportunity to estimate how a management alternative impacts greenhouse gas emissions at a large regional scale. We integrated a process-based model, DNDC, with a GIS database of paddy area, soil properties, and management factors. We simulated soil carbon sequestration (or net CO2 emission) and CH4 and N2O emissions from China\u27s rice paddies (30 million ha), based on 1990 climate and management conditions, with two water management scenarios: continuous flooding and midseason drainage. The results indicated that this change in water management has reduced aggregate CH4 emissions about 40%, or 5 Tg CH4 yr−1, roughly 5–10% of total global methane emissions from rice paddies. The mitigating effect of midseason drainage on CH4 flux was highly uneven across the country; the highest flux reductions (\u3e200 kg CH4-C ha−1 yr−1) were in Hainan, Sichuan, Hubei, and Guangdong provinces, with warmer weather and multiple-cropping rice systems. The smallest flux reductions (\u3c25 kg CH4-C ha−1 yr−1) occurred in Tianjin, Hebei, Ningxia, Liaoning, and Gansu Provinces, with relatively cool weather and single cropping systems. Shifting water management from continuous flooding to midseason drainage increased N2O emissions from Chinese rice paddies by 0.15 Tg N yr−1 (∼50% increase). This offset a large fraction of the greenhouse gas radiative forcing benefit gained by the decrease in CH4 emissions. Midseason drainage-induced N2O fluxes were high (\u3e8.0 kg N/ha) in Jilin, Liaoning, Heilongjiang, and Xinjiang provinces, where the paddy soils contained relatively high organic matter. Shifting water management from continuous flooding to midseason drainage reduced total net CO2emissions by 0.65 Tg CO2-C yr−1, which made a relatively small contribution to the net climate impact due to the low radiative potential of CO2. The change in water management had very different effects on net greenhouse gas mitigation when implemented across climatic zones, soil types, or cropping systems. Maximum CH4 reductions and minimum N2O increases were obtained when the mid-season draining was applied to rice paddies with warm weather, high soil clay content, and low soil organic matter content, for example, Sichuan, Hubei, Hunan, Guangdong, Guangxi, Anhui, and Jiangsu provinces, which have 60% of China\u27s rice paddies and produce 65% of China\u27s rice harvest

Transcriptomic response of Saccharomyces cerevisiae to octanoic acid production

The medium-chain fatty acid octanoic acid is an important platform compound widely used in industry. The microbial production from sugars in Saccharomyces cerevisiae is a promising alternative to current non-sustainable production methods, however, titers need to be further increased. To achieve this, it is essential to have in-depth knowledge about the cell physiology during octanoic acid production. To this end, we collected the first RNA-Seq data of an octanoic acid producer strain at three time points during fermentation. The strain produced higher levels of octanoic acid and increased levels of fatty acids of other chain lengths (C6-C18) but showed decreased growth compared to the reference. Furthermore, we show that the here analyzed transcriptomic response to internally produced octanoic acid is notably distinct from a wild type\u27s response to externally supplied octanoic acid as reported in previous publications. By comparing the transcriptomic response of different sampling times, we identified several genes that we subsequently overexpressed and knocked out, respectively. Hereby we identified RPL40B, to date unknown to play a role in fatty acid biosynthesis or medium-chain fatty acid tolerance. Overexpression of RPL40B led to an increase in octanoic acid titers by 40%

Treatment of cyclic vomiting syndrome with co-enzyme Q10 and amitriptyline, a retrospective study

<p>Abstract</p> <p>Background</p> <p>Cyclic vomiting syndrome (CVS), which is defined by recurrent stereotypical episodes of nausea and vomiting, is a relatively-common disabling condition that is associated with migraine headache and mitochondrial dysfunction. Co-enzyme Q10 (Co-Q) is a nutritional supplement that has demonstrated efficacy in pediatric and adult migraine. It is increasingly used in CVS despite the complete lack of studies to demonstrate its value in treatment</p> <p>Methods</p> <p>Using an Internet-based survey filled out by subjects with CVS or their parents, the efficacy, tolerability and subject satisfaction in CVS prophylaxis were queried. Subjects taking Co-Q (22 subjects) were compared against those taking amitriptyline (162 subjects), which is the general standard-of-care.</p> <p>Results</p> <p>Subjects/parents reported similar levels of efficacy for a variety of episode parameters (frequency, duration, number of emesis, nausea severity). There was a 50% reduction in at least one of those four parameters in 72% of subjects treated with amitriptyline and 68% of subjects treated Co-Q. However, while no side effects were reported on Co-Q, 50% of subjects on amitriptyline reported side effects (P = 5 × 10^-7), resulting in 21% discontinuing treatment (P = 0.007). Subjects/parents considered the benefits to outweigh the risks of treatment in 47% of cases on amitriptyline and 77% of cases on Co-Q (P = 0.008).</p> <p>Conclusion</p> <p>Our data suggest that the natural food supplement Co-Q is potentially efficacious and tolerable in the treatment of CVS, and should be considered as an option in CVS prophylaxis. Our data would likely be helpful in the design of a double-blind clinical trial.</p

ACVR2 (activin receptor type 2)

Review on ACVR2 (activin receptor type 2), with data on DNA, on the protein encoded, and where the gene is implicated

Elasticity model of a supercoiled DNA molecule

Within a simple elastic theory, we study the elongation versus force characteristics of a supercoiled DNA molecule at thermal equilibrium in the regime of small supercoiling. The partition function is mapped to the path integral representation for a quantum charged particle in the field of a magnetic monopole with unquantized charge. We show that the theory is singular in the continuum limit and must be regularised at an intermediate length scale. We find good agreement with existing experimental data, and point out how to measure the twist rigidity accurately.Comment: Latex, 4 pages. The figure contains new experimental data, giving a new determination of the twist rigidit

Time-Dependent Physicochemical Changes of Carbonate Surfaces from SmartWater (Diluted Seawater) Flooding Processes for Improved Oil Recovery.

Over the past few decades, field- and laboratory-scale studies have shown enhancements in oil recovery when reservoirs, which contain high-salinity formation water (FW), are waterflooded with modified-salinity salt water (widely referred to as the low-salinity, dilution, or SmartWater effect for improved oil recovery). In this study, we investigated the time dependence of the physicochemical processes that occur during diluted seawater (i.e., SmartWater) waterflooding processes of specific relevance to carbonate oil reservoirs. We measured the changes to oil/water/rock wettability, surface roughness, and surface chemical composition during SmartWater flooding using 10-fold-diluted seawater under mimicked oil reservoir conditions with calcite and carbonate reservoir rocks. Distinct effects due to SmartWater flooding were observed and found to occur on two different timescales: (1) a rapid (&lt;15 min) increase in the colloidal electrostatic double-layer repulsion between the rock and oil across the SmartWater, leading to a decreased oil/water/rock adhesion energy and thus increased water wetness and (2) slower (&gt;12 h to complete) physicochemical changes of the calcite and carbonate reservoir rock surfaces, including surface roughening via the dissolution of rock and the reprecipitation of dissolved carbonate species after exchanging key ions (Ca2+, Mg2+, CO32-, and SO42- in carbonates) with those in the flooding SmartWater. Our experiments using crude oil from a carbonate reservoir reveal that these reservoir rock surfaces are covered with organic-ionic preadsorbed films (ad-layers), which the SmartWater removes (detaches) as flakes. Removal of the organic-ionic ad-layers by SmartWater flooding enhances oil release from the surfaces, which was found to be critical to increasing the water wetness and significantly improving oil removal from carbonates. Additionally, the increase in water wetness is further enhanced by roughening of the rock surfaces, which decreases the effective contact (interaction) area between the oil and rock interfaces. Furthermore, we found that the rate of these slower physicochemical changes to the carbonate rock surfaces increases with increasing temperature (at least up to an experimental temperature of 75 °C). Our results suggest that the effectiveness of improved oil recovery from SmartWater flooding depends strongly on the formation of the organic-ionic ad-layers. In oil reservoirs where the ad-layer is fully developed and robust, injecting SmartWater would lead to significant removal of the ad-layer and improved oil recovery

Greenhouse gas emission consequences of large-scale changes in water management of China’s rice paddies during 1980-2000

In China, midseason paddy drainage, which reduces growing season methane fluxes and enhances growing season nitrous oxide fluxes, was first implemented in the early 1980s, and has gradually replaced continuous flooding in much of the paddy rice area. We constructed a prediction rice paddy methane and nitrous oxide emissions in China using the DNDC biogeochemical model. Results of continuous flooding and midseason drainage simulations for all paddy fields in China (about 30 million ha) were combined with regional scenarios for the timing of the transition from continuous flooding to predominantly mid-season drainage to generate estimates of total methane (CH4) and nitrous oxide (N2O) flux for 1980-2000. By shifting from continuous flooding to midseason drainage management, we estimate that total N2O emissions from the rice paddies in China increased by about 0.17 Tg N2O-N yr−1 due to the stimulated nitri- fication and denitrification, while CH4 emissions decreased by about 4.5 Tg CH4-C yr−1 due to increased soil aeration. Simulated net carbon loss in paddy soils was about 0.65 Tg C yr−1 due to elevated decomposition. On a 100-year time frame, CH4 has a global warming potential (GWP) 23 times that of CO2, and N2O has a GWP 296 times that of CO2. The total GWP impact (2000 vs. 1980) of shifting to predominantly mid-season paddy drainage was -0.138 Pg CO2-equiv yr−1 from methane, +0.077 Pg CO2-equiv yr−1 from nitrous oxide and +0.0024 Pg CO2 yr−1 from soil C loss, for a total GWP impact of -0.059 Pg CO2-equiv yr−1 . The results imply that more than half of the GWP benefit of decreased CH4 emissions was offset, primarily by increases in N2O emissions, and to a small degree by soil C loss