Visualization and quantitation of spine deformity in zebrafish models of scoliosis by micro-computed tomography

Summary: Zebrafish (Danio rerio) are increasingly used to investigate spine development, growth, and for studying the etiology of spinal deformity, such as scoliosis. Here, we present a micro-computed tomography-based pipeline for visualizing the zebrafish skeleton. We describe steps for sample preparation, imaging, data management, and processing. We then detail analysis of vertebral and spine morphology using open-source software. This protocol will be useful for scientists using zebrafish to understand spine development and disease.For complete details on the use and execution of this protocol, please refer to Bearce et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics

Control Growth of \u3ci\u3eEuphorbia pulcherrima\u3c/i\u3e Willd. ex Klotzsch ‘Sonora Jingle’ and ‘Sonora White’ Using Ethephon

Ethephon was sprayed on Euphorbia pulcherrima Willd. ex Klotzsch (poinsettia) ‘Sonora Jingle’ and ‘Sonora White’ to control their height and produce more compact potted plants. The results showed that Ethephon could effectively control the growth of ‘Sonora Jingle’ and ‘Sonora White’ poinsettia. Height was reduced by was 33.4 ± 0.8% for ‘Sonora Jingle’ and 30.8 ± 1.3% for ‘Sonora White’ poinsettia, when 700 mg L−1 Ethephon was sprayed three times on 29 August, 20 September, and 13 October 2005, respectively. Similar to other plant growth retardants, side effects including phytotoxicity and delays to first bract color were also observed on Ethephon treated poinsettia. However, all plants produced were still of a marketable quality