6 research outputs found

    Выделение и характеристика митохондриальной ДНК-топоизомеразы I типа из проростков кукурузы (Zea mays)

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    Проведена очистка и изучены биохимические характеристики ДНК-топоизомеразы типа I из митохондрий проростков кукурузы. Установлено, что фермент проявляет свойства, характерные для ДНК-топоизомераз как эукариотического, так и прокариотического происхождения.Здійснено очистку та вивчено біохімічні характеристики ДНК-топоізомерази типу 1 з мітохондрій проростків кукурудзи. Встановлено, що згаданий фермент виявляє властивості, характерні для ДНК-топоізомераз як еукаріотичного, так і прокаріотичного походження.The type I DNA-topoisomerase has been isolated and purified by procedures of salting out and ion chromatography from maize seedling mitochondria. It has been found that the enzyme examined possesses properties characteristic of DNA-topoisomerases of both eukaryotic and prokaryotic origin

    Characterisation of the Gradient Coatings TiN/(Ti,Al,Si)N/TiN Type Deposited on Sintered Tool Materials

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    The paper presents results of the structural examinations, tests of mechanical and working properties ofthin wear resistant gradient coatings of the TiN/(Ti,Al,Si)N/TiN type, deposited in the CAE process ontothe substrate from the cermets and cemented carbides. Structural examinations are presented of theapplied coatings and their substrate made on the SEM, TEM and on the LM.Evaluation of the adhesion of the deposited coatings onto the cemented carbides and cermets was madeusing the scratch test. Cutting properties of the investigated materials were determined basing on thetechnological continuous cutting tests of the C45E steel. Substrate hardness tests and microhardness testsof the deposited coatings were made on the ultra-micro-hardness tester at 70 mN load. Surface roughnesstests were also made before depositing the coatings and after completing the PVD process
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