<i>Ex vivo</i> anti-HPV16-E2 peptide IFNγ ELISPOT responses in women presenting with usual VIN.

<p>IFNγ ELISPOT response to E2 peptide pools was studied using PBMCs from A: women presenting with usual VIN either during the whole study (F#1 and F#7) or at study entry and up to the healing after treatment (F#3, F#6, F#8 before M6, F#5 before M12) and B: asymptomatic women having cleared their usual VIN after treatment (F#2, F#4 before their entry in the study, F#3, F#6, F#8 from M6, F#5 from M12). Bars represent numbers of SFC/10⁶ PBMCs against a pool of E2 peptides, negative (0) and positive (+) controls obtained at M0 (blue), M6 (red), M12 (yellow) and M18 (turquoise blue). Standard deviation of triplicates appears for each bar. Responses were considered significant when the mean number of SFC per 10⁶ cells in the 3 experimental wells was >3-fold the mean number of SFC in the negative control wells (PBMC alone) and >30 SFC/10⁶ cells. Positive responses are identified by a black star above the corresponding bar.</p

Intracellular cytokine synthesis to HPV16 E2 peptides in proliferative T-cell female responders.

<p>The percentage of CD4+ T-cells synthesizing single IFNγ, dual IFNγ/IL2 or single IL2 was measured in response to E2 peptide pools or E2 peptide and compared to negative (no or irrelevant peptide) and positive controls (pool of non-HPV viral peptides). Single IFNγ responses are shown in blue, dual IFNγ/IL2 in red and single IL2 in green. Percentages of CD4+ responding cells are in the same color as the cytokine(s) synthesized. The white color represents the percentage of non responding cells among CD4+ T lymphocytes. Responses were considered as positive when the percentage of CD4+ T-cells synthesizing cytokines increased by at least 0.2% in the presence of the peptide as compared to negative control.</p

Clinical and biological characteristics of the eight women having usual VIN and their male partners.

<p>NA: not applicable.</p

Anti-HPV16 E2 peptide proliferative responses in women.

<p>T-cell proliferation induced by E2 peptide pools were studied with PBMCs from A: women presenting with usual VIN either during the whole study (F#1 and F#7) or at study entry and up to the healing after treatment (F#3, F#6, F#8 before M6, F#5 before M12) and B: asymptomatic women having cleared their usual VIN after treatment (F#2, F#4 before their entry in the study, F#3, F#6, F#8 from M6, F#5 from M12). SI is represented by the cpm in peptide-stimulated cells/cpm in negative control wells (without peptide). Each bar represents SI against a pool of E2 peptide or positive control (+) obtained from PBMCs sampled at M0 (blue), M6 (red), M12 (yellow) and M18 (turquoise blue). Proliferative responses with SI >3 were scored as positive, provided that cpm in the negative control was above 500. The values of positive SI for E2 pools are indicated in black near the corresponding bar. Responses to 15 mer peptides comprised in the various peptide pools are shown, superposed to the response to the pool, in another color. The SI values for these peptides are indicated using the same colors near the corresponding bars.</p

<i>Ex vivo</i> anti-HPV16-E2 peptide IFNγ ELISPOT responses in male partners of women presenting with usual VIN.

<p>IFNγ ELISPOT responses to E2 peptide pools were studied with PBMCs from male partners of women presenting with usual VIN. Bars represent numbers of SFC/10⁶ PBMCs against a pool of E2 peptides, negative (0) and positive (+) controls obtained at M0 (blue), M6 (red), M12 (yellow) and M18 (turquoise blue). Standard deviation of triplicates appears for each bar. Responses were considered significant when the mean number of SFC per 10⁶ cells in the 3 experimental wells was >3-fold the mean number of SFC in the negative control wells (PBMC alone) and >30 SFC/10⁶ cells. Positive responses are identified by a black star above the corresponding bar.</p

Ex vivo IFNγ ELISPOT responses in women and men against HPV16, HPV2, HPV27 and HPV55 E2 309–323 peptide.

<p>A: number of SFC in negative control was substracted.</p><p>B: number of SFC was <3-fold the mean number of SFC in the negative control.</p

Anti-HPV16 E2 peptide proliferative responses in male partners of women presenting with usual VIN.

<p>Proliferative assays were performed using PBMCs in presence of pools of E2 peptides. SI is represented by the cpm in peptide-stimulated cells/cpm in negative control wells (without peptide). Each bar represents SI against a pool of E2 peptide or positive control (+) obtained from PBMCs sampled at M0 (blue), M6 (red), M12 (yellow) and M18 (turquoise blue). Proliferative responses with SI >3 were scored as positive, provided that cpm in the negative control was above 500. The values of positive SI for E2 pools are indicated in black near the corresponding bar. Responses to 15 mer peptides comprised in the various peptide pools are shown, superposed to the response to the pool, in another color. The SI values for these peptides are indicated in the same colors near the corresponding bars.</p

Peptides and pools spanning the whole E2 protein.

<p>Peptides and pools spanning the whole E2 protein.</p