Avaliação da expressão gênica em pacientes com Diabetes Mellitus tipo 2

O Diabetes Mellitus caracteriza-se como um grupo de doenças metabólicas, marcado pela hiperglicemia resultante de defeitos na secreção de insulina, em sua ação ou em ambos. O aparecimento da doença está relacionado a fatores de risco genéticos, ambientais e comportamentais, com aumento da prevalência em todo o mundo. Com relação à expressão gênica e Diabetes Mellitus tipo 2 (DM2), informação muito limitada foi encontrada enfocando-se genes relacionados ao sistema imune. O objetivo deste estudo foi avaliar o efeito da descompensação do DM2 sobre a expressão de genes do sistema imune, tais como IL10RB, IL10RA, IL10, JAK1, STAT3, SOCS3, IP10 e ICAM1. A amostra foi constituída por 60 pacientes divididos em 2 grupos: Grupo 1 (DM2 Compensado metabolicamente) e Grupo 2 (DM2 Descompensado metabolicamente). Toda a amostra foi submetida a exame físico e avaliação laboratorial para verificação da glicemia de jejum, hemoglobina glicada (HbA1c), insulina e proteína C-reativa. De cada paciente foi coletado sangue em tubo com EDTA, sendo separados os leucócitos para investigar a expressão gênica. Após a extração de RNA total do sangue dos pacientes de ambos os Grupos, foi confeccionado o cDNA e a expressão gênica foi avaliada por qPCR (Reação em Cadeia da Polimerase em Tempo Real, ou quantitativo). Como resultado da expressão gênica comparando os Grupos 1 e 2, obtiveram-se diferenças significantes para os genes IL10RA, JAK1, STAT3, SOCS3 e ICAM1. Todos esses genes se mostraram mais expressos no Grupo 2 ...Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

Dyslipidemia rather than Type 2 Diabetes Mellitus or Chronic Periodontitis Affects the Systemic Expression of Pro-and Anti-Inflammatory Genes

A high percentage of type 2 diabetes mellitus (T2D) patients are also affected by dyslipidemia and chronic periodontitis (CP), but no studies have determined the gene expression in patients that are simultaneously affected by all three diseases. We investigated the systemic expression of immune-related genes in T2D, dyslipidemia, and CP patients. One hundred and fifty patients were separated into five groups containing 30 individuals each: (G1) poorly controlled T2D with dyslipidemia and CP; (G2) well-controlled T2D with dyslipidemia and CP; (G3) normoglycemic individuals with dyslipidemia and CP; (G4) healthy individuals with CP; (G5) systemic and periodontally healthy individuals. Blood analyses of lipid and glycemic profiles were carried out. The expression of genes, including IL10, JAK1, STAT3, SOCS3, IP10, ICAM1, IFNA, IFNG, STAT1, and IRF1, was investigated by RT-qPCR. Patients with dyslipidemia demonstrated statistically higher expression of the IL10 and IFNA genes, while IFNG, IP10, IRF1, JAK1, and STAT3 were lower in comparison with nondyslipidemic patients. Anti-inflammatory genes, such as IL10, positively correlated with parameters of glucose, lipid, and periodontal profiles, while proinflammatory genes, such as IFNG, were negatively correlated with these parameters. We conclude that dyslipidemia appears to be the primary disease that is associated with gene expression of immune-related genes, while parameters of T2D and CP were correlated with the expression of these important immune genes

Dyslipidemia rather than Type 2 Diabetes Mellitus or Chronic Periodontitis Affects the Systemic Expression of Pro- and Anti-Inflammatory Genes

A high percentage of type 2 diabetes mellitus (T2D) patients are also affected by dyslipidemia and chronic periodontitis (CP), but no studies have determined the gene expression in patients that are simultaneously affected by all three diseases. We investigated the systemic expression of immune-related genes in T2D, dyslipidemia, and CP patients. One hundred and fifty patients were separated into five groups containing 30 individuals each: (G1) poorly controlled T2D with dyslipidemia and CP; (G2) well-controlled T2D with dyslipidemia and CP; (G3) normoglycemic individuals with dyslipidemia and CP; (G4) healthy individuals with CP; (G5) systemic and periodontally healthy individuals. Blood analyses of lipid and glycemic profiles were carried out. The expression of genes, including IL10, JAK1, STAT3, SOCS3, IP10, ICAM1, IFNA, IFNG, STAT1, and IRF1, was investigated by RT-qPCR. Patients with dyslipidemia demonstrated statistically higher expression of the IL10 and IFNA genes, while IFNG, IP10, IRF1, JAK1, and STAT3 were lower in comparison with nondyslipidemic patients. Anti-inflammatory genes, such as IL10, positively correlated with parameters of glucose, lipid, and periodontal profiles, while proinflammatory genes, such as IFNG, were negatively correlated with these parameters. We conclude that dyslipidemia appears to be the primary disease that is associated with gene expression of immune-related genes, while parameters of T2D and CP were correlated with the expression of these important immune genes