56 research outputs found
Massa específica foliar de Maytenus ilicifolia Mart. ex Reiss. em função de variáveis ambientais.
Resumo
Variação dos teores foliares de silício, taninos e lignina, em Maytenus ilicifolia Martius (Espinheira-santa), em função de variáveis ambientais e genéticas.
Organizado por Patricia Póvoa de Mattos, Celso Garcia Auer, Rejane Stumpf Sberze, Katia Regina Pichelli e Paulo César Botosso
Reatividade de silicatos.
Projeto/Plano de Ação: 01.09.10103-04
PRNT de escórias na correção da acidez do solo para o cultivo da alfafa.
projeto/Plano de Ação: 0109.01.001
Teor de metais pesados na cultura da aveia preta em função da aplicação superficial de resíduos urbanos e industriais, no sistema plantio direto.
Projeto/Plano de Ação: 01.09.10103-04
Dinâmica do nitrogênio em função da aplicação de lodos de esgoto em sistema de plantio direto.
Projeto/Plano de Ação: 01.09.10103-04
Identification of global inhibitors of cellular glycosylation
Small molecule inhibitors of glycosylation enzymes are valuable tools for dissecting glycan functions and potential drug candidates. Screening for inhibitors of glycosyltransferases are mainly performed by in vitro enzyme assays with difficulties moving candidates to cells and animals. Here, we circumvent this by employing a cell-based screening assay using glycoengineered cells expressing tailored reporter glycoproteins. We focused on GalNAc-type O-glycosylation and selected the GalNAc-T11 isoenzyme that selectively glycosylates endocytic low-density lipoprotein receptor (LDLR)-related proteins as targets. Our screen of a limited small molecule compound library did not identify selective inhibitors of GalNAc-T11, however, we identify two compounds that broadly inhibited Golgi-localized glycosylation processes. These compounds mediate the reversible fragmentation of the Golgi system without affecting secretion. We demonstrate how these inhibitors can be used to manipulate glycosylation in cells to induce expression of truncated O-glycans and augment binding of cancer-specific Tn-glycoprotein antibodies and to inhibit expression of heparan sulfate and binding and infection of SARS-CoV-2
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