Pediatric Chest Ultrasound for Bedside Diagnosis of Pneumonia: A Validation Study for Diagnostic Options in Developing Countries

Background: Pneumonia remains a major cause of morbidity and mortality in childhood with a higher burden in developing countries. Diagnosis relies on clinical findings with supporting evidence from chest X-ray (CXR) and occasionally chest computed tomography (CT). Aim of the work: The aim of this study was to assess the role of lung ultrasonography (LUS) for bedside diagnosis of pediatric pneumonia in comparison to chest CT as a gold standard. Methods: The study was performed on 50 children admitted to Cairo University Children’s Hospitals. All children presented with the classical clinical picture of pneumonia and did CXR, LUS and chest CT. Results: Radiological findings demonstrated lung consolidation in 48 patients (96%) by CXR, 49 (98%) patients by LUS and all 50 patients (100%) by CT chest. Pleural effusion was found in 8 (16%) patients by CT chest, all of which were detected by LUS (100%), but only 5 were correctly detected by CXR (specificity 62.5%) in addition to 3 false positive cases. LUS detected pleurisy in 29 children (58%) (specificity 100%) while X-rays did not. Also 70% of children needed sedation before CT scanning as compared to none for LUS. Conclusion: Lung ultrasound is a sensitive, specific, safe and available tool that can be used by clinicians in cases of suspected pneumonia. It is more reliable than CXR, does not require sedation, and can be repeated at the patient's bedside with no risk of irradiation

ISOLATION AND IDENTIFICATION OF Spiroplasma citri USING SOME SENSITIVE METHODS

Spiroplasma citri, the causal agent of stubborn disease, was isolated from leaves and fruits of diseased citrus plants and cultured on solid and liquid C3G medium. On the basis of mode of transmission, symptomatology, shape on solid medium, staining of infected plant tissues with Dienes’ stain and phase contrast microscopy, the isolated agent was identified as Spiroplasma citri. Identifiction was ensured by ELISA and PCR techniques. A fragment (1053 bp) from the spiralin gene of S. citri was amplified by PCR using two specific primers for the spiralin gene. The nucleic acid hybridization techniques (Southern and dot blots) were used for identification of the spiroplasmal genome using non-radioactive DNA probe specific for spiralin gene region. It was carried out as an alternative sensitive method for rapid detection of the Egyption isolate of S. citri   using non-radioactive DNA probe specific for spiralin gene region

Unveiling the therapeutic potential of exogenous β-hydroxybutyrate for chronic colitis in rats: novel insights on autophagy, apoptosis, and pyroptosis

Ulcerative colitis (UC) is a chronic relapsing inflammatory disease of the colorectal area that demonstrates a dramatically increasing incidence worldwide. This study provides novel insights into the capacity of the exogenous β-hydroxybutyrate and ketogenic diet (KD) consumption to alleviate dextran sodium sulfate (DSS)-induced UC in rats. Remarkably, both interventions attenuated disease activity and colon weight-to-length ratio, and improved macro and microstructures of the damaged colon. Importantly, both β-hydroxybutyrate and KD curbed the DSS-induced aberrant NLRP3 inflammasome activation as observed in mRNA and protein expression analysis. Additionally, inhibition of the NLRP3/NGSDMD-mediated pyroptosis was detected in response to both regimens. In parallel, these modalities attenuated caspase-1 and its associated consequences of IL-1β and IL-18 overproduction. They also mitigated apoptosis as indicated by the inactivation of caspase-3. The anti-inflammatory effects of BHB and KD were confirmed by the reported decline in the levels of inflammatory markers including MPO, NFκB, IL-6, and TNF-α. Moreover, these interventions exhibited antioxidative properties by reducing ROS production and improving antioxidative enzymes. Their effectiveness in mitigating UC was also evident in the renovation of normal intestinal epithelial barrier function, as shown by correcting the discrepancies in the levels of tight junction proteins ZO-1, OCLN, and CLDN5. Furthermore, their effects on the intestinal microbiota homeostasis were investigated. In terms of autophagy, exogenous β-hydroxybutyrate upregulated BECN-1 and downregulated p62, which may account for its superiority over KD in attenuating colonic damage. In conclusion, this study provides experimental evidence supporting the potential therapeutic use of β-hydroxybutyrate or β-hydroxybutyrate-boosting regimens in UC

Anatomical Variants of the Sciatic Nerve Division in the Pelvis, the Gluteal Region and the Thigh: A Cadaveric Study

Introduction: Anatomical dissimilarities can be found in any part of the body and is pertinent to identify patients who are not treated successfully with standard medical care. Anatomical variants of the Sciatic Nerve (SN) were defined many years ago, and may have consequences in certain diseases, as sciatica. Specialised care may be required to these patients due to their unique anatomy. Aim: To study the cadaveric anatomical variants of the SN divisions in the pelvis, the gluteal region and the thigh and also to describe its linkage to the Piriformis Muscle (PM). Materials and Methods: This descriptive study was conducted from June 2021 to September 2021 in the Department of Anatomy of Qassim University, Saudi Arabia. Total 30 lower limbs and gluteal regions of formalin fixed male cadavers were used to study the anatomical variants of the SN division into its terminal branches and its relation to the PM. Numbers and percentages of the specimens falling in the groups were calculated and tabulated. Results: Out of 30, 3 specimens (10%), showed division of the SN in the pelvis, where both Tibial Nerve (TN) and Common Fibular Nerve (CFN) course separately below the piriformis muscle. While, 8 specimens (26.66%) showed division of the SN in the pelvis, where CFN pierces the piriformis muscle, and tibial nerve lies below it. About 5 specimens (16.66%), showed division of the sciatic nerve into tibial nerve and CFN in the gluteal region at the level of the obturator internus muscle. Additionally, 9 specimens (30%) showed division of the sciatic nerve into tibial nerve and CFN occurred in the upper of the posterior aspect of the thigh. Lastly, 5 specimens (16.66%), revealed division of the sciatic nerve into tibial nerve and CFN in the middle of the posterior aspect of the thigh. Conclusion: The most prevalent level of bifurcation of the SN in the present study was the upper part of the posterior aspect of the thigh (30%), while the least common level of the SN bifurcation was in the pelvis, before its exits in the gluteal region, where TN and CFN course separately below PM (10%).</jats:p

Podocyte-related biomarkers' role in evaluating renal toxic effects of silver nanoparticles with the possible ameliorative role of resveratrol in adult male albino rats

Extensive uses of silver nanoparticles (Ag NPs) in different industries result in exposure to these nanoparticle imperatives in our daily lives. Resveratrol is found in many plants as a natural compound. The present study aimed to estimate the renal toxic effects of Ag NPs in adult male albino rats and the underlying relevant mechanisms while studying the possible role of resveratrol in ameliorating these effects. Thirty adult albino rats were split into 5 groups; control, vehicle, resveratrol (30 mg/kg), Ag NPs (300 mg/kg), and resveratrol + Ag NPs groups. The treatments were given orally for 4 weeks. Ag NPs group displayed a reduction in kidney weight ( absolute and relative), excess in urinary levels of kidney injury molecule, neutrophil gelatinase-associated lipocalin, cystatin, and blood kidney biomarkers (creatinine, urea, and potassium), increases in oxidative stress markers with the reduction in antioxidant markers, and decreases in serum sirtuin 1(SIRT1) level. Upregulation of interleukin 1 beta, tumor necrosis factor-alpha, and monocyte chemoattractant protein-1 gene expressions with downregulation of nephrin and podocin gene expressions in renal tissues were also observed. These changes were associated with histological alterations of the glomeruli and tubules, and increased area percentage of collagen fiber. A significant increase in the optical density of transforming growth factor-beta 1 and claudin-1 immunostaining was detected in the Ag NPs group when compared to other groups. All these changes were alleviated by the usage of resveratrol through its anti-oxidant, anti-inflammatory, and activation of SIRT1 recommending its use as a renoprotective agent

Virtual dissection applications in learning human anatomy: international medical students’ perspectives

Abstract Background Understanding human anatomy is fundamental to building medical competencies. This study explored the diverse perceptions of medical students in Saudi Arabia and Egypt concerning virtual dissection applications. Methods Students from different Saudi and Egyptian governmental universities were invited. The online questionnaire covered the sociodemographic characteristics, students’ perception of the advantages of virtual anatomy applications, satisfaction with their efficiency, and their recommendation to use them. Comparisons were performed in country-wise and then university-wise patterns. Significance was considered with a P < 0.05. Results The mean age of 401 participants was 20 ± 1.6. Females were 63% and the males were 37%. Saudi universities participated by 47% and Egyptian universities by 53% with various academic levels. The traditional program covers 25% of the participants and 75% are in integrated programs. Virtual dissection applications were used by 84% of the sample while the remaining 16% did not use them. No significant differences between Saudi participants and those from Egypt in the adopted teaching methods and the usual use of virtual dissection applications. However, significant differences in considering the ease of virtual applications (P = 0.016), their perfectness in lectures (P = 0.04), and their perception (P = 0.034). Also, there was no significant difference in satisfaction while their recommendation differed significantly (P = 0.034). The university-wise comparisons elaborated significant differences in the feasibility, reality, usefulness, preference, and perfectness of virtual dissection applications in lecturing. Conclusion Technology can be an efficient complementary, not a replacement resource to support the educational process. Also, students’ perceptions are valuable in maximizing the educational benefits of curricula