Molekularna filogenija i karakterizacija mundri ovce (Ovis aries) u Pakistanu sekvenciranjem mitohondrijskog citokroma b i podjedinice I citokrom oksidaze

The main focus of this research is to determine the molecular phylogeny and characterization of Mundri Sheep (Ovis aries) through sequencing of mitochondrial Cytochrome b and Cytochrome Oxidase Subunit I (COI). This sheep breed appears morphologically different from other local sheep breeds of Pakistan. The current research is carried out to appraise the status of Mundri sheep whether it is aa different breed from other breeds or not. Blood samples of Mundri sheep were collected from Livestock Experiment Station, (LES) Fazilpur in district Rajanpur (Punjab). DNA was isolated and subjected to Polymerase Chain Reaction (PCR) for amplification of Cytochrome b and COI genes using appropriate primers. PCR products were sequenced and analyzed by MEGA X software. The phylogeny analysis categorized Ovis aries including Mundri sheep into three and two groups for Cytochrome b and COI genes respectively. It showed Mundri sheep as a separate group and thus as a separate breed from all other local sheep breeds. Hence the study validates based on Cytochrome b and COI that Mundri sheep is a distinctive breed from the rest of the local sheep breed.Glavni je cilj ovog istraživanja bio odrediti molekularnu filogeniju i karakterizaciju mundri ovce (Ovis aries) sekvenciranjem mitohondrijskog citokroma b i podjedinice I citokrom oksidaze (COI). Ova se pasmina ovaca morfološki čini drugačijom od ostalih lokalnih pasmina ovaca u Pakistanu. Ovo je istraživanje provedeno da bi se procijenio status mundri ovce, da bismo mogli odrediti radi li se o pasmini drugačijoj od ostalih pasmina. Uzorci krvi mundri ovce prikupljeni su iz Stanice za eksperimente na stoci (engl. Livestock Experiment Station – LES) Fazilpur u okrugu Rajanpur (Punjab). DNK je izolirana i podvrgnuta lančanoj reakciji polimerazom (PCR) zbog pojačanja citokrom B i COI gena uporabom prikladnih primera. PCR proizvodi su sekvencirani i analizirani pomoću MEGA X softvera. Filogenetska analiza kategorizirala je Ovis aries uključujući mundri ovcu, u tri i dvije skupine za citokrom b, odnosno COI gene. Istraživanje je pokazalo da je mundri ovca posebna skupina i time zasebna pasmina ovaca u odnosu na ostale lokalne pasmine. Na temelju citokroma b i COI, naša je studija potvrdila da je mundri ovca zasebna pasmina i da se razlikuje od ostalih lokalne pasmine ovaca