Forensic analysis of the Turkey 2023 presidential election

Concerns about the integrity of Turkey's elections have increased with the recent transition from a parliamentary democracy to an executive presidency under Recep Tayyip Erdogan. Election forensics tools are used to identify statistical traces of certain types of electoral fraud, providing important information about the integrity and validity of democratic elections. Such analyses of the 2017 and 2018 Turkish elections revealed that malpractices such as ballot stuffing or voter manipulation may indeed have played a significant role in determining the election results. Here, we apply election forensic statistical tests for ballot stuffing and voter manipulation to the results of the first round of the 2023 presidential election in Turkey. We find that the 2023 elections exhibit similar statistical irregularities to those observed in the 2018 presidential elections, however the magnitude of these distortions has decreased. We estimate that 2.4% (SD 1.9%) of electoral units may have been affected by ballot-stuffing practices in favour of Erdogan, compared to 8.5% (SD 3.9%) in 2018. We also find that areas with few and smaller polling stations had significantly inflated vote and turnout numbers, again, in favour of Erdogan. Our results suggest that Turkish elections continue to be riddled with statistical irregularities, which may be indicative of electoral fraud

Binding ability properties of β-cyclodextrin dimers linked through their secondary faces towards cancer chemotherapeutic agent methotrexate

The binding ability properties of two β-cyclodextrin dimers linked through their secondary faces by short, rigid spacer arms towards the cancer chemotherapeutic agent methotrexate were studied by ITC and NMR (1D and ROESY) experiments. Both dimers are able to bind two molecules of methotrexate with a binding constant between 2.4 and 3.5 times higher than that for native β-cyclodextrin, the dimer having the shortest linker forming the most stable complex

A non‐covalent “click chemistry” strategy to efficiently coat highly porous MOF nanoparticles with a stable polymeric shell

Background Metal-organic framework nanoparticles (nanoMOFs) are biodegradable highly porous materials with a remarkable ability to load therapeutic agents with a wide range of physico-chemical properties. Engineering the nanoMOFs surface may provide nanoparticles with higher stability, controlled release, and targeting abilities. Designing postsynthetic, non-covalent self-assembling shells for nanoMOFs is especially appealing due to their simplicity, versatility, absence of toxic byproducts and minimum impact on the original host-guest ability. Methods In this study, several β-cyclodextrin-based monomers and polymers appended with mannose or rhodamine were randomly phosphorylated, and tested as self-assembling coating building blocks for iron trimesate MIL-100(Fe) nanoMOFs. The shell formation and stability were studied by isothermal titration calorimetry (ITC), spectrofluorometry and confocal imaging. The effect of the coating on tritium-labeled AZT-PT drug release was estimated by scintillation counting. Results Shell formation was conveniently achieved by soaking the nanoparticles in self-assembling agent aqueous solutions. The grafted phosphate moieties enabled a firm anchorage of the coating to the nanoMOFs. Coating stability was directly related to the density of grafted phosphate groups, and did not alter nanoMOFs morphology or drug release kinetics. Conclusion An easy, fast and reproducible non-covalent functionalization of MIL-100(Fe) nanoMOFs surface based on the interaction between phosphate groups appended to β-cyclodextrin derivatives and iron(III) atoms is presented. General significance This study proved that discrete and polymeric phosphate β-cyclodextrin derivatives can conform non-covalent shells on iron(III)-based nanoMOFs. The flexibility of the β-cyclodextrin to be decorated with different motifs open the way towards nanoMOFs modifications for drug delivery, catalysis, separation, imaging and sensing. This article is part of a Special Issue entitled “Recent Advances in Bionanomaterials” Guest Editors: Dr. Marie-Louise Saboungi and Dr. Samuel D. Bader

β‑Cyclodextrin-Bearing Gold Glyconanoparticles for the Development of Site Specific Drug Delivery Systems

Three novel gold nanoparticles containing multiple long, flexible linkers decorated with lactose, β-cyclodextrin and both simultaneously have been prepared. The interaction of such nanoparticles with β-D-galactose-recognizing lectins peanut agglutinin (PNA) and human galectin-3 (Gal-3) was demonstrated by UV-Vis studies. Gal-3 is well-known to be overexpressed in several human tumors and can act as biorecognizable target. This technique also allowed us to estimate their loading capability toward the anticancer drug methotrexate (MTX). Both results make these glyconanoparticles potential site-specific delivery systems for anticancer drugs

The effects of citalopram and low-dose risperidone on memory and anxiety in rats subjected to chronic immobilization stress

OBJECTIVES: Many clinical reports describe the beneficial effects of low-dose atypical antipsychotic added to the antidepressants in the management of anxiety disorders. The aim of this study was to evaluate the effect of low-dose atypical antipsychotic when added to antidepressant treatment on cholinergic M1 receptor expression in the hippocampus and amygdale region in learning and cognitive disorders caused by anxiety. METHODS: The treatments were administered by using different test models on memory, learning, and anxiety, as well as the effect on muscarinic M1 receptor expression levels were assessed. Citalopram (10 mg/kg/sc) and combination [citalopram and risperidone (1 mg/kg/sc)] treatments were applied after stress induction using the immobilization model in rats. Animals groups were randomly divided as: control, stress, stress + citalopram, and stress + combination treatment group. Rats in stress groups were immobilized in cages for 4 h a day for 15 days. On days 1–5, groups were subjected to Morris water maze (MWM), open field, and elevated plus maze (EPM) tests. RESULTS: MWM test results have shown that citalopram induces an anxiolytic effect. Low-dose risperidone treatment has increased the antidepressant-like activity of citalopram in all tests. In OFT the number of squares that rats were circulating on the plane was increased and the time spent by the rats on the maze platform was also increased in MWM. In addition to this, the time spent by the rats on the open arms of the EPM test were also increased. Since the combined treatment increased the discovery of the environment and the active behaviour in tests; all those reflected the increase in general activity. Findings also suggest that treatments may play an effective role in altering the expression level of M1 receptors which are effective in learning and recalling information in the amygdale and the hippocampus. Combination treatment has been shown to provide a meaningful correction of stress-induced memory and learning functions. CONCLUSIONS: These findings indicate that combination treatment may help reduce the stress-induced impairments in cognitive functions

Epidural anesthesia may attenuate lipid peroxidation during aorto-femoral surgery

Purpose: To determine the effect of epidural anesthesia (FP) on oxygenation of the chronically ischemic limb in patients undergoing aorto-femoral bypass grafting and to assess whether it produces an alteration of lipid peroxidation and antioxidant status following revascularization