The growth of plant embryos in vitro. Preliminary experiments on the role of accessory substances

The aseptic culture of plant embryos isolated from the seed dates back to the work of Brown and Morris,(1) Hannig(2) and Dietrich.(3) More recent contributions to our knowledge concerning the culture in vitro of excised embryos have been made by Tukey,(4) Brunner(5) and LaRue,(6) among others. It has been recognized by, for example, Ray(7) that the embryo culture technique offers a useful tool for biochemical investigations, and it has also been recognized(4,8) that it may be used as a practical measure to circumvent the abortion of embryos. It has, however, been found that in general the growth of the excised embryo, even upon a medium containing essential inorganic materials and sugar, is far less than that of normal intact seedlings. This has led to the suggestion(5) that "accessory growth factors" which are needed in minute amounts, are required by the developing plant as they are by the developing animal organism. The present work, as well as that of Kogl and Haagen-Smit,(9) furnish final proof that this is the case; that these accessory substances, although normally furnished by the seed, may be replaced to some extent by pure compounds added in small amounts to the embryo culture medium. These investigations, taken up early in 1936, are concerned particularly with orienting experiments undertaken with an ultimate view toward the elucidation of the nature and mode of action of these accessory growth factors. The embryo culture technique is here to be used as a tool in the "hormonal" analysis of plant development