EFFICACY AND SAFETY OF BISOPROLOL IN YOUNG MEN WITH ARTERIAL HYPERTENSION AND OBESITY

Aim. To evaluate effect of bisoprolol on circadian blood pressure (BP) profile, autonomic cardiovascular regulation, lipid and carbohydrate metabolism in young patients with arterial hypertension (HT) and obesity. Materials and methods. 36 men aged 18 to 35 years with HT 1-2 degrees, obesity 1-2 degrees and signs of sympathetic over activity were examined. In addition to standard clinical examination assessment of heart rate variability (HRV) during orthostatic test with spectral analysis, ambulatory BP monitoring, evaluation of lipid profile, oral glucose tolerance test, determination of immunoreactive insulin, insulin resistance index and functional activity of beta-cells were performed. Anthropometric measurements included body mass index and waist circumference. Bisoprolol in the initial dose of 5 mg QD orally for 12 weeks was assigned to all patients. After 12 weeks of bisoprolol treatment repeated examination was performed. Results. Significant changes in HRV after 12 weeks of treatment with bisoprolol was found. Parasympathetic influence of autonomic nervous system increased during orthostatic test. Contribution of the slow and very slow waves, which reflect sympathetic and neurohumoral activity , reduced. Total spectral power was significantly increased. Initial tachycardia reduced. Target BP values were achieved after 12 weeks of treatment with bisoprolol 5 mg QD. Increase in a number of dippers and decrease in a number of nondippers in terms of both systolic and diastolic BP was observed. Heart rate and HRV was significantly decreased. No adverse events in terms of lipid or carbohydrate metabolism were found during treatment with bisoprolol. Conclusion. Bisoprolol 12 week treatment resulted in sympathetic activity decrease and parasympathetic activity increase, achievement of BP target levels, improvement of BP circadian profile. Bisoprolol therapy in dose of 5 mg/day demonstrated metabolic neutrality in patients with risk factors of cardiovascular diseases and type 2 diabetes

CHANGE IN CONTENT OF GLYCOPROTEINS ON THE SURFACE OF ENDOTHELIAL CELL CULTURE EA.HY 926 AND INTIMA OF INTERNAL CAROTID ARTERIES UNDER THE INFLUENCE OF MAGNESIUM OROTATE

Aim. To study changes in the glycoproteins content in the glycocalyx of endothelial cells under the influence of magnesium orotate.Material and methods. Endotheliocytes of cell culture EA.hy 926 were examined before  and after addition of the magnesium orotate to the culture medium. Intimal endothelium of internal carotid arteries, fragments of which were obtained by sequential execution of bilateral resection of the arteries before  and after course of treatment with magnesium orotate was also investigated. Anthony’ method (1931), designed for the study of glycoproteins in the bacteria capsule and adapted by L.V. Didenko (2013) for eukaryotes was used to detect glycoproteins. The scanning electron microscopy with simultaneous x-ray microanalysis was applied.Results. Total number of Cu2+-signals per one scanning field in the mapping of samples for Cu2+ as the indirect index of proteoglycan content in EA.hy 926 endothelial cell culture before incubation with magnesium orotate was 6928±124, and after incubation with magnesium orotate – 7592±131. Number of Cu2+-signals on the surface of the internal carotid artery intima before treatment with magnesium orotate was 5015±407, and after treatment – 6100±152 per one scanning field.Conclusion. A significant increase in the content of glycoproteins on the surface of EA.hy926 endothelial cell culture (+10%) and internal carotid artery intima (+22%) under the influence of magnesium orotate was found

nanoparticles production and inclusion in s aureus incubated with polyurethane an electron microscopy analysis

This study shows that submicron/nanoparticles found in bacterial cells (S. aureus) incubated with polyurethane (a material commonly used for prostheses in odontostomatology) are a consequence of biodestruction. The presence of polyurethane nanoparticles into bacterial vesicles suggests that the internalization process occurs through endocytosis. TEM and FIB/SEM are a suitable set of correlated instruments and techniques for this multi facet investigation: polyurethane particles influence the properties of S. aureus from the morpho-functional standpoint that may have undesirable effects on the human body. S. aureus and C. albicans are symbiotic microorganisms; it was observed that C. albicans has a similar interaction with polyurethane and an increment of the biodestruction capacity is expected by its mutual work with S. aureus

Direct EPR Detection of Nitric Oxide in Mice Infected with the Pathogenic Mycobacterium Mycobacterium tuberculosis

It has been shown that treatment of mice preinfected with Mycobacterium tuberculosis with spin NO traps (iron complexes with diethyldithiocarbamate) enables detection of large amounts of NO in internal organs 2 and 4 weeks after infection (up to 55–57 μmol/kg of wet lung tissue accumulated with spin NO traps during 30 min). The animals were infected with the drug-sensitive laboratory strain H37Rv and a clinical isolate nonrespondent to antituberculous drugs (the multidrug-resistant strain of M. tuberculosis) obtained from a patient with an active form of tuberculosis. Two weeks after infection with the multidrug-resistant strain, the NO level in the lungs, spleen, liver and kidney increased sharply concurrently with slight lesions of lung tissue. A reverse correlation, i.e., low level of NO in the lungs and other internal organs and extensive injury of lung tissue, was established for H37Rv-infected mice. Four weeks after infection, NO production in the lungs increased dramatically for both M. tuberculosis strains resulting in 80–84% damage of lung tissue. The lesion is suggested to be due to the development of defense mechanisms in M. tuberculosis counteracting NO effects

Circadian dynamics of lipid profile in Polar North oil industry workers with arterial hypertension

The study was focused on circadian dynamics of lipid profile in Polar North oil industry shift-workers with arterial hypertension (AH). In total, 95 AH patients (Group I - 53 individuals working daytime; Group II - 42 participants working night-time), were examined. Significantly higher levels of total cholesterol (TC), low and very low-density lipoprotein cholesterol (LDL, VLDL-C), triglycerides (TG), as well as decreased high-density С (HDL-C) level, were observed in night-working participants. AH in night-working Polar North inhabitants is an additional risk factor of atherosclerosis

DYNAMICS OF STRUCTURAL AND FUNCTIONAL STATUS OF MYOCARDIUM DUE TO COMBINATION THERAPY WITH AMLODIPINE AND BISOPROLOL IN PATIENTS WITH ARTERIAL HYPERTENSION

Aim. To evaluate the effect of 6-month therapy with combination of amlodipine and bisoprolol on the structural and functional status of the myocardium in hypertensive patients who work in the Far North.Material and methods. 140 hypertensive patients who live in the Khanty-Mansiysk Autonomous District - Yugra were divided into two groups depending on arrangement of working time. The first group included 72 patients who work only day shift; the second group – 68 patients who work alternate (day/night) shifts. Combination therapy with amlodipine and bisoprolol assigned to all patients. Echocardiography was performed at baseline, after 12 weeks, and after 6 months of therapy.Results. The target blood pressure (BP) level in group 1 was achieved in 92.9%. A share of patients with normal left ventricular (LV) geometry increased from 37.5 to 44.8%; a share of patients with concentric and eccentric LV hypertrophy (LVH) decreased from 30.6 to 23.9% and 19.4 to 19.2%, respectively. Target BP level in group 2 was achieved in 87.9%. A share of patients with normal LV geometry increased from 23.5 to 33.3%; while share of patients with concentric and eccentric LVH decreased from 45.6 to 38.1% and from 19.1 to 17.4%, respectively. A positive correlation between LV myocardial index and average daily systolic and diastolic BP was found.Conclusion. Revealed changes in BP and in LV structure and function due to treatment with amlodipine and bisoprolol can be considered as cardioprotective effect of this combination in hypertensive patients who work in the Far North. This effect was more pronounced in hypertensive patients working alternate (day/night) shifts

EFFECTIVENESS OF ANTIHYPERTENSION THERAPY IN PATIENTS WITH ARTERIAL HYPERTENSION WORKING IN FAR NORTH

Aim. To evaluate combination treatment effectiveness by amlodipine with bisoprolol (A/B) on the data of 24-hour BP pattern in patients with arterial hypertension (AH) working in Far North.Material and methods. Totally 140 patients studied with AH who were separated into 2 groups according to their working conditions: 1st group — 72 persons, working only daytime; 2nd group — 68 shift workers. All patients were prescribed combination therapy A/B with final dosages: 1st group 8,3±1,3/9,8±1,6 mg daily, 2nd group 9,5±1,5/14,5±1,2 mg daily. 24-hour BP values were studied at baseline, in 4 weeks and in 6 weeks of the treatment.Results. In combination 6-months therapy by A/B in the 2nd group of AH patients comparing to the 1 st groupthere was lower rate of target BP achievement (87,9 and 92,9%, resp.). The "pressure load" parameters did not reach normal ranges in both groups, however were more significant in the 2nd group, that is why it is important to prescribe the 3rd hypotensive medication to these patients. We found BP variability normalization and improvement of morning BP dynamics in both groups that decreases the risk of cardiovascular complications. There was good tolerability

FIVE-YEAR DYNAMICS OF CIRCADIAN BLOOD PRESSURE PROFILE IN FAR NORTH WORKERS WITH ARTERIAL HYPERTENSION

Aim. In a prospective study, to assess the dynamics of circadian profiles of blood pressure (BP) in Far North workers with arterial hypertension (AH) and different shift working patterns. Material and methods. In the first phase of the study (2003–2005), 95 patients with Stage 1–2 AH were examined. Group I included 53 patients who worked only day shifts; Group II included 42 patients who worked alternating day and night shifts. In the second phase (2007–2011), both groups were examined, including 24-hour BP monitoring, and two subgoups (treated vs. untreated AH) were defined. Results. In Far North workers with AH, there was a negative dynamics in circadian BP profile, particularly among night shift workers. AH patients require antihypertensive combination therapy irrespective of their shift working patterns