Assessment of serum metallograms in patients with purine urolithiasis before and after treatment

Investigations on the homeostasis of the hydroelectrolytic metabolism is important in the assessment of the uroconcrements’ biogenesis and of the therapeutic effects (based on diverse procedures). Our investigations have been performed on patients with purine urolithiasis included in two groups, according to the applied treatment for the removal of the uroconcrements. A group included 23 patients who underwent surgical treatment and the other group included 18 patients treated with Extracorporeal Shock-Wave Lithotripsy (ESWL). Before and after the therapeutic procedure the serum metallograms of the main alkaline (Na, K) and alkaline-earth (Ca, Mg) metals were determined by using spectrophotometry. The obtained results revealed post-treatment changes in the serum metallogram as follows: increase of natriemia and calcemia; decrease of kaliemia and magnesiemia in both groups. The find data show the dyshomeostasis of the main alkaline and alkaline-earth metals after the treatment underlying their implication in urolithogenesis

Effects of ethanol on the hepatic DNA in materno-fetal complex and on the serum proteins in pregnant female rats

Experimental investigations on the action of ethanol were performed on pregnant female rats and their fetuses. The Wistar strain pregnant rats were included into an experimental (E) and a control (C) group. Animals of experimental group received ethanol and thecontrol group consumed tap water. At the end of the experiment blood samples and liver samples were taken for analysis. In the present study there were pursued values of some biochemical parameters, i.e. maternal and fetal hepatic DNA concentration, maternal serumproteins and electrophoretic fractions (albumins as well as α-, β- and γ-globulins). The obtained data revealed a statistically non-signifiant increase of hepatic DNA both in mothers and fetuses of experimental group. Serumproteins concentration in the pregnant rats showed a significant increase. Regarding the electrophoretic fractions, a decrease of albumins and increase of globulins were observed . As to globulin subfractions a hyper-α-globulinemia, hypo-β- and hypo-γ-globulinemia were revealed