ELK1 Gene Transfection Effect in Prostate Cancer Cell Line Proliferation Activity

Three type of prostate cancer cell line was selected for this study (PC3, DU145 & LNCaP) as a model, transfected by liposome with the ELK1 gene ( control & knock down) , then detecting the proliferation ability of the cultured cell lines by the Mtt or (proliferation) assay that shows clear effect of ELK1 gene in this cells comparing with the control cell transfected with the knock down gene, using for each type of cells 6 repeats ,for each type there was two groups 1st for control ELK1 and the 2nd was knock down or (sh) ELK1, all works was done in Johns Hopkins University / School of Medicine / Pathology Department ( MD, USA

The Elk1 gene effect on prostate cancer cell line wound healing ability

In this study prostate cancer cell line model was used selecting three type of them (Pc3, DU145 & LNCaP) , gene transfecting was done by using the liposome into the cell culture cells then detecting the healing ability by the Wound Healing or (scratching) test that shows clear effect of ELK1 gene in this cells comparing with the control cell transfected with the knock down gene, using for each type of cells 6 repeats ,for each type there was two groups 1st for control ELK1 and the 2nd was knock down or (sh) ELK1, all works was done in Johns Hopkins University / School of Medicine / Pathology Department ( MD, USA

Network Pharmacology, Molecular Docking, and Molecular Dynamics Simulation Analysis Reveal Insights into the Molecular Mechanism of <i>Cordia myxa</i> in the Treatment of Liver Cancer

Traditional treatments of cancer have faced various challenges, including toxicity, medication resistance, and financial burdens. On the other hand, bioactive phytochemicals employed in complementary alternative medicine have recently gained interest due to their ability to control a wide range of molecular pathways while being less harmful. As a result, we used a network pharmacology approach to study the possible regulatory mechanisms of active constituents of Cordia myxa for the treatment of liver cancer (LC). Active constituents were retrieved from the IMPPAT database and the literature review, and their targets were retrieved from the STITCH and Swiss Target Prediction databases. LC-related targets were retrieved from expression datasets (GSE39791, GSE76427, GSE22058, GSE87630, and GSE112790) through gene expression omnibus (GEO). The DAVID Gene Ontology (GO) database was used to annotate target proteins, while the Kyoto Encyclopedia and Genome Database (KEGG) was used to analyze signaling pathway enrichment. STRING and Cytoscape were used to create protein–protein interaction networks (PPI), while the degree scoring algorithm of CytoHubba was used to identify hub genes. The GEPIA2 server was used for survival analysis, and PyRx was used for molecular docking analysis. Survival and network analysis revealed that five genes named heat shot protein 90 AA1 (HSP90AA1), estrogen receptor 1 (ESR1), cytochrome P450 3A4 (CYP3A4), cyclin-dependent kinase 1 (CDK1), and matrix metalloproteinase-9 (MMP9) are linked with the survival of LC patients. Finally, we conclude that four extremely active ingredients, namely cosmosiin, rosmarinic acid, quercetin, and rubinin influence the expression of HSP90AA1, which may serve as a potential therapeutic target for LC. These results were further validated by molecular dynamics simulation analysis, which predicted the complexes with highly stable dynamics. The residues of the targeted protein showed a highly stable nature except for the N-terminal domain without affecting the drug binding. An integrated network pharmacology and docking study demonstrated that C. myxa had a promising preventative effect on LC by working on cancer-related signaling pathways