Improving the Final Liveweight and Growing Ability of TETRA-H a Dual Purpose Chicken Type by Using a New Experimental Sire Line

Our study was carried out with the pure, crossed and reciprocal crossed off spring of TETRA-H hybrid, a coloured slow-growing broiler genotype which was developed in the 1980’s by Babolna TETRA Ltd. In this project the potential of a new, improved sire line was also investigated. Experiments were carried out in the Poultry Test Station of the Kaposvar University in 2008 - 2010. In the first stage, growing ability of TETRA-H and a commercial genotype (standard control=Shaver Redbro) had been compared. In stages two and three the LL line, which was previously used as sire line, the newly selected sire Line EE, and the off spring came from the combination of those lines have been centrally tested. A total number of 6453 birds were used. The following traits were investigated: live weight, body composition evaluated by CT, carcass yield, meat quality, etc. The positive impact of the newly developed sire line (EE) in their off spring was confirmed. Lower slaughter weight of the F1 off spring has improved by 10.1-10.8%, making the newly developed hybrid highly competitive on the market. Furthermore, the crossing method was incompetent (LL♂xEE♀ or EE♂xLL♀) in the birds live weight and growing ability

Increased Expression of Claudin-1 and Claudin-7 in Liver Cirrhosis and Hepatocellular Carcinoma.

Claudins have been reported to be differentially regulated in malignancies and implicated in the process of carcinogenesis and tumor progression. Claudin-1 has been described as key factor in the entry of hepatitis C virus (HCV) into hepatocytes and as promoter of epithelial-mesenchymal transition in liver cells. The objective of the current study was to characterize claudin expression in hepatocellular carcinoma (HCC) as well as HCC-surrounding and normal liver samples with respect to cirrhosis and HCV infection. Expression of claudin-1, -2, -3, -4, and -7 was measured by morphometric analysis of immunohistochemistry, and Western blotting in 30 HCCs with 30 corresponding non-tumorous tissues and 6 normal livers. Claudin-1 and -7 protein expression was found significantly elevated in cirrhosis when compared with non-cirrhotic liver. HCCs developed in cirrhotic livers showed even higher expression of claudin-1 contrary to decreased claudin-7 expression when compared with cirrhosis. With reference to HCV status, HCCs or surrounding livers of HCV-infected samples did not show significant alterations in claudin expression when compared with HCV-negative specimens. Cirrhotic transformation associates with elevated claudin-1 and -7 expressions in both non-tumorous liver and HCC. The fact that no significant differences in claudin expression were found regarding HCV-positivity in our sample set suggests that HCV infection alone does not induce a major increase in the total amount of its entry co-factor claudin-1. Increased expression of claudin-1 seems to be a consequence of cirrhotic transformation and might contribute to a more effective HCV entry and malignant transformation

Erratum to: Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) (Autophagy, 12, 1, 1-222, 10.1080/15548627.2015.1100356

non present