10 research outputs found
Production and partial characterization of interleukin 2 induced by periodic acid oxidation of lymphocyte membranes
Murine splenocytes, when stimulated to undergo blastogenesis by H
5IO
6 oxidation, produced a lymphokine with a spectrum of properties identical to that generally ascribed to IL-2 released by lectin-stimulated cells. These included the sustained propagation of IL-2-dependent CT6 cells, as well as identical thermal and enzymatic stabilities. Cell membrane carbonyls generated
in situ by the oxidation of cell membranes served as triggers for subsequent IL-2 production by the activated cells. Reduction of membrane carbonyls by NaBH
4, and their addition reaction with NaHSO
3 and NH
2OH abrogated cell activation and inhibited, but did not abolish, IL-2 production. None of the specific carbonyl reagents, e.g., NaBH
4, NaHSO
3, and NH
2OH, have by themselves induced blastogenic transformation, although they did elicit IL-2 production. It is therefore concluded that cell membrane carbonyls serve as triggers for IL-2 production by H
5IO
6-transformed cells, although an increased rate of DNA synthesis per se is not an indispensable precondition for IL-2 synthesis