Sodium Current by Hindlimb Unloading

This study aimed to characterize the excitability changes in peripheral motor axons caused by hindlimb unloading (HLU), which is a model of disuse neuromuscular atrophy. HLU was performed in normal 8-week-old male mice by fixing the proximal tail by a clip connected to the top of the animal's cage for 3 weeks. Axonal excitability studies were performed by stimulating the sciatic nerve at the ankle and recording the compound muscle action potential (CMAP) from the foot. The amplitudes of the motor responses of the unloading group were 51% of the control amplitudes [2.2 ± 1.3 mV (HLU) vs. 4.3 ± 1.2 mV (Control), P = 0.03]. Multiple axonal excitability analysis showed that the unloading group had a smaller strength-duration time constant (SDTC) and late subexcitability (recovery cycle) than the controls [0.075 ± 0.01 (HLU) vs. 0.12 ± 0.01 (Control), P < 0.01; 5.4 ± 1.0 (HLU) vs. 10.0 ± 1.3 % (Control), P = 0.01, respectively]. Three weeks after releasing from HLU, the SDTC became comparable to the control range. Using a modeling study, the observed differences in the waveforms could be explained by reduced persistent Na+ currents along with parameters related to current leakage. Quantification of RNA of a SCA1A gene coding a voltage-gated Na+ channel tended to be decreased in the sciatic nerve in HLU. The present study suggested that axonal ion currents are altered in vivo by HLU. It is still undetermined whether the dysfunctional axonal ion currents have any pathogenicity on neuromuscular atrophy or are the results of neural plasticity by atrophy

Use of the index of pulmonary vascular disease for predicting long-term outcome of pulmonary arterial hypertension associated with congenital heart disease

AimsLimited data exist on risk factors for the long-term outcome of pulmonary arterial hypertension (PAH) associated with congenital heart disease (CHD-PAH). We focused on the index of pulmonary vascular disease (IPVD), an assessment system for pulmonary artery pathology specimens. The IPVD classifies pulmonary vascular lesions into four categories based on severity: (1) no intimal thickening, (2) cellular thickening of the intima, (3) fibrous thickening of the intima, and (4) destruction of the tunica media, with the overall grade expressed as an additive mean of these scores. This study aimed to investigate the relationship between IPVD and the long-term outcome of CHD-PAH.MethodsThis retrospective study examined lung pathology images of 764 patients with CHD-PAH aged &lt;20 years whose lung specimens were submitted to the Japanese Research Institute of Pulmonary Vasculature for pulmonary pathological review between 2001 and 2020. Clinical information was collected retrospectively by each attending physician. The primary endpoint was cardiovascular death.ResultsThe 5-year, 10-year, 15-year, and 20-year cardiovascular death-free survival rates for all patients were 92.0%, 90.4%, 87.3%, and 86.1%, respectively. The group with an IPVD of ≥2.0 had significantly poorer survival than the group with an IPVD &lt;2.0 (P = .037). The Cox proportional hazards model adjusted for the presence of congenital anomaly syndromes associated with pulmonary hypertension, and age at lung biopsy showed similar results (hazard ratio 4.46; 95% confidence interval: 1.45–13.73; P = .009).ConclusionsThe IPVD scoring system is useful for predicting the long-term outcome of CHD-PAH. For patients with an IPVD of ≥2.0, treatment strategies, including choosing palliative procedures such as pulmonary artery banding to restrict pulmonary blood flow and postponement of intracardiac repair, should be more carefully considered

都市部の妊娠期・出産後の女性の喫煙と食生活および生活習慣との関連

都市部に在住する乳児の母親を対象として,妊娠前後の喫煙の現状と食・生活習慣との関連を明らかにすることを目的に,無記名自記式アンケート調査を実施した。対象は,平成14年11月の1ヶ月間に,京都市内14ヶ所の保健所・支所にて,生後4ヶ月児健診を受診した991名の乳児の母親である。喫煙や食・生活習慣に関する調査紙を手渡し,回答が得られた466名(回収率47.0%)を解析対象とした。その結果,妊娠前喫煙率は28.5%で,年齢が若いほど喫煙率が高かった。妊娠を契機に74.4%が禁煙し,妊娠中喫煙率は7.3%であったが,出産後喫煙率は10.9%に増加していた。喫煙者は非喫煙者と比較して,食・生活習慣,授乳,喫煙に関する知識の欠如などの健康課題を有しており,小児期からの喫煙防止教育等,総合的な保健対策が望まれる

活性化したCreERT2は、染色体組み換え異常を起こすことで造血障害をきたす

京都大学0048新制・課程博士博士(医学)甲第15027号医博第3403号新制||医||978(附属図書館)27488京都大学大学院医学研究科内科系専攻(主査)教授 前川 平, 教授 武田 俊一, 教授 長澤 丘司学位規則第4条第1項該当Doctor of Medical ScienceKyoto UniversityDA

日本の短期留学プログラムにおけるフランスの日本語学習者‐学習者対象のアンケート調査結果が示唆すること (French Learners of Japanese on Japanese Short Term Study Abroad Programs –Examining the Results from the Survey of Students

International audienc

日本の短期留学プログラムにおけるフランスの日本語学習者‐学習者対象のアンケート調査結果が示唆すること (French Learners of Japanese on Japanese Short Term Study Abroad Programs –Examining the Results from the Survey of Students

International audienc

日本の短期留学プログラムにおけるフランスの日本語学習者‐学習者対象のアンケート調査結果が示唆すること (French Learners of Japanese on Japanese Short Term Study Abroad Programs –Examining the Results from the Survey of Students

International audienc

日本と海外の日本語教育機関の教育連携の模索‐短期交換留学プログラムの学習者アンケートから (Towards a Japanese Language pedagogy network of Home and Host universiteis – observations for a survey of Japanese Language Learners),

International audienc