Influence of recombinant erythropoietin on mesenchymal stem cells proliferation and differentiation during cultivation on allogenic bone matrix

Effectiveness of mesenchymal stem cells (MSCs) proliferation and differentiation was studied after addition of medicinal product containing human recombinant erythropoietin (rhEPO) into enriched culture medium with components for osteo- and angiogenesis. Cultivation of mesenchymal stem cells under static conditions using “cocktail” of growth factors and rhEPO allowed to populate allogenic discs-scaffolds with connective tissue formations with the participation of osteoblasts and elongated multicellular structures, which formed after endothelium-like cells fusio

Method of individual forecasting of sow reproductive performance on the basis of a non-linear canonical model of a random sequence

Improvement of sow reproductive performance is a key factor determining the efficiency of the pig production cycle and profitability of pork production. This article presents the solution of an important scientific and practical problem of individual forecasting of sow reproduction . The population used for the present study is from a pig farm managed by the Limited Liability Company (LLC) ‘Tavriys’kisvyni’ located in Skadovsky district (Kherson region, Ukraine). The experimental materials used for this study consisted of 100 inds. of productive parent sows of the Large White breed.The litter size traits – the total number of piglets born (TNB), number of piglets born alive (NBA) and number of weaned piglets (NW) – were monitored in the first eight parities over an eleven year period (2007–2017). The method of the forecasting of sow litter size is developed based on the non-linear canonical model of the random sequence of a litter size change. The proposed method allows us to take maximum account of stochastic peculiarities of sow reproductive performance and does not impose any restrictions on the random sequence of a litter size change (linearity, stationarity, Markov property, monotony, etc.). The block diagram of the algorithm presented in this work reflects the peculiarities of calculation of the parameters of a predictive model. The expression for the calculation of an extrapolation error allows us to estimate the necessary volume of a priori and a posteriori information for achieving the required quality of solving the forecasting problem. The results of the numerical experiment confirmed the high accuracy of the proposed method of forecasting of sow reproduction. The method offered by us almost doubles the accuracy of forecasting of sow litter size compared to the use of the Wiener and Kalman methods. Thus, average forecast error decreases across the range of features TNB (1.71), NBA (1.68) and NW (1.25 piglets). Apparently, this may reflect a higher level of manifestation of the genetically determined level of individual sow fertility at the moment of piglet weaning. The higher adequacy of the developed mathematical model with regard to NW can be also due to the fact that the relations between sow litter size in different farrowings primarily have a non-linear character, which is taken into maximum account in our offered model. Given non-linearity, on the other hand, turns out to be a significant factor determining a lower estimation of the repeatability value for NW compared to the estimations for TNB and NBA. The use of the developed method will help to improve the efficiency of pig farming

Myocardial revascularization by percutaneous coronary intervention in senile patients with chronic total occlusion: a modern view of the problem

In recent decades, the problem of coronary artery disease (CAD) accompanied by chronic total occlusion (CTO) has come to the fore in senile patients. The reason for this is the absence of generally accepted management strategies for these groups of patients. The choice of myocardial revascularization method in elderly patients has a fundamental impact on outcomes, but requires additional research. There is no doubt that in recent years, percutaneous coronary intervention (PCI) in CTO has gained a status as the first-choice method for this pathology. The following main reasons are distinguished: development of intravascular imaging; improvement of surgical instruments; development of new approaches to recanalization; increase in the number of experienced surgeons. The use of PCI for CTO in elderly patients is specified by the upward trend in the proportion of senile population worldwide. This review considers the validity and potential of using PCI for CTO in senile patients, as well as its safety, benefits and prospects. For this review, the following databases were used: Medline (PubMed), RSCI (eLIBRARY), Google Scholar