The Importance of Analytical Chemistry in Therapeutic Drug Monitoring for Personalized Medicine

Personalized therapy (PM) has the potential to adapt treatment with the best response and highest safety to provide better patient care. Key data is drug concentration of biological materials such as plasma and serum.Individual drug therapy means, choice of a drug and its dose regime should fit every individual specifically. Thus efficacy of a drug treatment would improve significantly. When developing an analytical method for (Therapeutic drug monitoring) TDM, it is important to choose a clinically relevant calibration range. This quantitation range should be built around the proposed target concentration, covering majority of samples as seen in the clinic (Ciocan-Cartita et al. 2019).Inter-individual variability in Pharmacokinetic variables may affect the blood concentration of drug so TDM approaches could solve the dosing problem.To achieve individual drug therapy with a reasonably predictive outcome, one must further account for different patterns of drug response among geographically and ethnically distinct populations. Keywords: LC-MS/MS, Therapeutic Drug Monitoring, Lenalidomide, Anastrozole DOI: 10.7176/CMR/12-7-05 Publication date:September 30th 202

A rapid liquid chromatography/tandem mass spectrometry method for simultaneous determination of levodopa, carbidopa, entacapone and their six related compounds in film-coated tablets

Rationale A liquid chromatography/tandem mass spectrometry (LC/MS/MS) method has been developed and validated to determine levodopa, carbidopa, entacapone, and corresponding six related substances - levodopa impurity B, levodopa impurity C, methyldopa, methylcarbidopa, entacapone impurity C, and entacapone impurity A - in film-coated tablets for the first time

Differential Pulse Voltammetric Determination of Anticancer Drug Regorafenib at a Carbon Paste Electrode: Electrochemical Study and Density Functional Theory Computations

Electrochemical properties of regorafenib (REG) were studied in 0.1 M Britton-Robinson buffer-methanol solutions (3 : 2, v/v) with pH between 3 and 8 at carbon paste electrode by cyclic and differential pulse voltammetry. The results exhibited irreversible anodic peak at about 0.90 V vs. Ag/AgCl, NaCl (3 M). The anodic peak was found to be diffusion-adsorption controlled. Mechanism of REG electrochemical reaction was studied by performing density functional theory computations and mass spectrometric analysis. A validated differential pulse voltammetry (DPV) technique for REG determination was performed. The calibration curve of REG on carbon paste electrode was linear in the concentration range of 0.5-13 mu g/mL and limit of detection was 0.10 mu g/mL. The recommended DPV method was used to detect REG in spiked plasma and urine specimens and average recoveries were 94%

Beroe ovata (Bosc,1802)' nın yağ asidi kompozisyonu

Marmara Denizinin kıyı bölgelerinden toplanan Beroe ovata'''nın toplam lipid miktarı 0.98±0.05 mg/g (n=6) olarak tespit edildi. Beroe ovata'nın yağ asidi bileşenleri gaz kromatografısi-kütle spektrometresi ile tayin edildi. Toplam doymuş, tek ve çok doymamış yağ asidi yüzdeleri sırasıyla 35.78, 14.11 ve 37.09 olarak bulundu. Eikozapentaenoik asit (EPA, 20:5n-3) ve dokozahekzaenoik asit (DHA, 22:6n-3) miktarları sırasıyla 9.75% ve 23.01% olarak bulundu. Dokozapentaenoik asit Marmara Dehizi'nde bulunan Beroe ovata'da bulunmadı. EPA/DHA oranı 0.42 olarak bulundu. Bu çalışmada Beroe ovata ile literatürde kayıtlı diğer Beroe türlerinin yağ asitlerinin içeriği incelenmiş ve bunlar arasında yalnız Beroe ovata' nın 21:0 ve 17:ln-7 yağ asitleri içerdiği tespit edilmiştir.Bu Beroe ovata'nın yağ asidi kompozisyonu üzerine ilk çalışmadır.Beroe ovata was collected from Kumkapi, Istanbul, Sea of Marmara. Fatty acids composition of Beroe ovgta were determined using GC/MS. Total lipid amount was found as 0.98±0.05 mg/g (n=6). Total saturated; monounsaturated and polyunsaturated fatty acids percentages were 35.78, 14.11 and 37.09, respectively. Eicosapentaenoic acid (EPA, 20:5n-3) percentage was 9.75 and docosahexaenoic acid (DHA, 22:6n-3) percentage was 23.01. Docosapentaenoic acid was not detected in Beroe ovata. EPA/DHA ratio was found as 0.42. The difference of fatty acids between the Beroe ovata with Beroe cucumis and Beroe forskalii were detection of 21:0 ve 17:ln-7 in only Beroe ovata This paper is the first study on the fatty acid composition of Beroe ovata