Grammar Translation Method In ELT: Hindering The Development Of Communicative Skills At Secondary Level

The purpose of this study is to evaluate the communication skill of students in English who were taught by Grammar Translation Method (GTM) at secondary school level in Pakistan. There is not usually any listening or speaking practice in GTM. Very little attention is placed on pronunciation or any communicative aspect of language. The skill exercised is reading and then only in the context of translation. Examinations also consist of translation of classical text. A questionnaire was used to collect data from15 teachers of Govt. schools of district Faisalabad who were using this method in the schools. The quantitative data were analyzed by simple frequency count and percentage, and qualitative data through the description of the classroom observation and interview. Analysis of the data shows that GTM put more emphasis on learning English words, phrases, sentences and structure pattern; but it has failed in practical use of English in real life situation. Students learning through this method at secondary school certificate level Pakistan in are unable to communicate even a basic conversation in English. It is suggested that the teachers should adopt communicative approach in ELT so that the communicative ability of the students may be enhanced. KEYWORDS: Grammar translation method GTM) , ELT, ESL, L1 (First language,) L2 (Second language ), communication, secondary leve

Terrorism-Related Factors Affecting Youth Sports Participation in Various Districts of Balochistan, A Province Pakistan

This study was carried out to assess the variables affecting young people's engagement in sports in several Balochistan areas in Pakistan. The current study was conducted in degree-granting institutions for both men and women in six (06) randomly selected Balochistan districts. In all, 824 students participated in the study 512 men and 312 women, or 10% of the students at 18 colleges for men and 14 colleges for women. A structured five-point Likert scale questionnaire that accepts responses of Strongly Agree (SA), Agree (A), Neither Disagree (NDA), Disagree (DA), and Strongly Disagree (SDA) was developed and used by the researcher. The researcher utilized the 0.05 significant level for the p-value to determine whether to accept or reject the provided hypotheses. The study’s conclusions demonstrated that Baluchistan’s law and order conditions, as well as terrorism, terrorism-related factors affecting youth sports participation in various districts of Balochistan, a province of Pakistan

Therapeutic Value of Medicinal Mushroom Agaricus blazei Murill

Agaricus blazei Murill (AbM) is a mushroom that has been utilized in alternative drug to anticipate cardiac disease, diabetes, arthritis, increased cholesterol, cancer, hepatitis and cancers. It contains β-glucan, which contributes to decreasing blood sugar in-vivo and acts against cancer. The extract of this mushroom exerts antioxidant action in-vivo. AbM contains agaritine that exerts antitumor effects against leukemic cells in vitro. This mushroom is also found to have immune-stimulatory function, tumor growth suppression, action against allergy, antiviral effects, antimicrobial function and immune modulatory effects. Researchers also studied its action in decreasing blood cholesterol. According to several studies, AbM containing (1→6)-β-D-glucan-exerts anti angiogenesis action. The purpose of this extensive review on the medicinal value of AbM mushroom was to highlight its significance and its traditional uses by scientific evidence to determine the effectiveness of the mushroom in various evidence-based uses. Google Scholar and PubMed search engines were used to browse articles from 1994-2019 on the therapeutic value of AbM. Initially, 74 articles were found related to the therapeutic value of AbM. After reviewing the available article, 42 were selected based on the medicinal uses of AbM. It was concluded that AbM possessed various bioactive compounds that are responsible for its therapeutic effects. Keywords: Mushroom; Antidiabetic; Therapeutics

Effectiveness of Natural Antioxidants against SARS-CoV-2? Insights from the In-Silico World

The SARS CoV-2 pandemic has affected millions of people around the globe. Despite many efforts to find some effective medicines against SARS CoV-2, no established therapeutics are available yet. The use of phytochemicals as antiviral agents provides hope against the proliferation of SARS-CoV-2. Several natural compounds were analyzed by virtual screening against six SARS CoV-2 protein targets using molecular docking simulations in the present study. More than a hundred plant-derived secondary metabolites have been docked, including alkaloids, flavonoids, coumarins, and steroids. SARS CoV-2 protein targets include Main protease (M(Pro)), Papain-like protease (PL(pro)), RNA-dependent RNA polymerase (RdRp), Spike glycoprotein (S), Helicase (Nsp13), and E-Channel protein. Phytochemicals were evaluated by molecular docking, and MD simulations were performed using the YASARA structure using a modified genetic algorithm and AMBER03 force field. Binding energies and dissociation constants allowed the identification of potentially active compounds. Ligand-protein interactions provide an insight into the mechanism and potential of identified compounds. Glycyrrhizin and its metabolite 18-β-glycyrrhetinic acid have shown a strong binding affinity for M(Pro), helicase, RdRp, spike, and E-channel proteins, while a flavonoid Baicalin also strongly binds against PL(pro) and RdRp. The use of identified phytochemicals may help to speed up the drug development and provide natural protection against SARS-CoV-2

Association between TSH status and prevalence of miscarriages and stillbirth

Thyroid hormones play a significant role in normal human body growth. Abnormalities in thyroid stimulating hormone (TSH) levels can result in pregnancy loss due to miscarriages and intrauterine death (IUD). The objective of the study was to assess the levels of association of thyroid stimulating hormone with miscarriages and IUD. The descriptive study involving 110 samples between 18-40 years of age fulfilling inclusion criteria were sampled for TSH testing (2ml blood) after attaining their written informed consent. The mean age of participants was 29.49±4.26 year. The prevalence of hypothyroidism and hyperthyroidism was 3.64% and 2.73%, respectively. Complications like gestational hypertension, depression and oligomenorrhea were found prevalent in these females. Majority of females were taking high/low iodine than recommended iodine level (150mcg). This work shows that there is a significant association between pregnancy loss and disturbed TSH levels among pregnant females

Process optimization for enhanced production of cellulases form locally isolated fungal strain by submerged fermentation

Cellulase has myriad applications in various sectors like pharmaceuticals, textile, detergents, animal feed and bioethanol production, etc. The current study focuses on the isolation, screening and optimization of fungal strain through one factor at a time technique for enhanced cellulase production.  In current study sixteen different fungal cultures were isolated and the culture which quantitatively exhibits higher titers of cellulase activity was identified both morphologically and molecularly by 18S rDNA and designated as Aspergillus niger ABT11. Different parameters like fermentation medium, volume, temperature, pH and nutritional components were optimized. The highest CMCase and FPase activities  was achieved in 100ml of M5 medium in the presence of 1% lactose and sodium nitrate at 30 oC, pH5 after 72 hours. The result revealed A. niger can be a potential candidate for scale up studies

Role of Surgical Feeding Gastrostomy in Patients Requiring Prolonged Nutrition in a Neurosurgical Department

Objective: To build up the nutritional status of the neurosurgical patients with an easy and practical way. Patients and Methods: This prospective study was conducted in Neurosurgery department of Pakistan Institute of Medical Sciences Islamabad in a six months’ time. In total thirty adult patients of either gender with H/O road traffic accident with severe head injury and GCS less than 8/15, persistent vegetative state, brain tumor patients who had developed lower cranial nerve palsies were included in the study. 28 for two way Foleys catheter was used in feeding gastrostomy. Results: Thirty patients, with the median age of 35 years (range, 18-55 years) underwent feeding gastrostomy. Before the gastrostomy tube insertion, 18 patients had enteral nutrition by a nasogastric tube and 10 had parenteral nutrition (PN), with a median duration of 14.5 (range, 4-60) and 12 (range, 7-25) days, respectively. Two patients accidentally pulled out the gastrostomy tubes 10 and 11 days after insertion. Buried bumper syndrome developed in 1 patient. Two patients died 8 and 34 days after the procedure in the neurosurgical ICU. Twenty-eight patients were discharged from the hospital while being fed via the gastrostomy tubes. In 11 patients who were able to resume oral feeding, the tube was removed, with a median interval of 62 (range, 25-150) days. There was no Procedure-related mortality. Conclusion: An improvised method of nutritional support according to our circumstances. This study can be extended to other surgical and medical patients who need nutritional support for longer period of time.&nbsp

Bioethanol production from urban cellulosic waste employing Alcaligenes faecalis HI-1 isolated from gut of termite Heterotermes indicola

This study assessed the potential of termite gut inhabiting bacteria towards bioconversion of cellulosic waste into biofuel. Total seven bacterial isolates from the gut of Heterotermes indicola were isolated. Among all the isolates, HI-1 produced the largest zone upon primary screening.  Untreated paper had more cellulose content (73.03%) than acid (0.5%) treated paper that was used as a lignocellulosic substrate for saccharification. Among all the isolates tested, glucose yield (1.08mg/mL) was high for HI-1 isolate. Several factors were considered for optimizing augmented glucose yield (8.57mg/mL) and growth (8.07×108cfu/mL), such as temperature 37°C, pH 4.5, 5% (w/v) substrate concentration, 6 % bacterial inoculum size, agitation 150 rpm with PEG 0.25 % and Ca2+ ions 0.002 g/L. Overall 8-fold increase in glucose yield was achieved. Enzyme activity of HI-1 showed higher endoglucanase 0.29 ± 0.01 (U/mL/min) and exoglucanase 0.15±0.01 (U/mL/min) activity under optimum conditions, mentioned above. temperature 37°C, pH 4.5, substrate concentration 5%, inoculum size 6%, surfactants PEG 0.01%, ions Ca2+(0.002g/L) and agitation (120 rpm). Simultaneous saccharification and fermentation (SSF) of hydrolyzed office paper yielded 5.43mg/mL bioethanol. According to 16S rRNA sequence homology, the bacterial isolate H1 was identified as Alcaligenes faecalis. Bioethanol production from office paper untreated waste proved an effective strategy. Bacteria having natural tendency towards cellulosic waste consumption are promising for bioconversion of cellulosic waste to valuable products

超好熱性アーキアにおけるキチン分解・資化系に関する研究

京都大学0048新制・課程博士博士(工学)甲第20712号工博第4409号新制||工||1685(附属図書館)京都大学大学院工学研究科合成・生物化学専攻(主査)教授 跡見 晴幸, 教授 森 泰生, 教授 浜地 格学位規則第4条第1項該当Doctor of Philosophy (Engineering)Kyoto UniversityDGA