Augmentation of ouabain sensitivity of rat liver Na/K‐ATPase by in vivo adenovirus‐mediated expression of the Na/K‐ATPase α2 subunit

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/117011/1/feb2s0014579397000872.pd

Costly choices for treating Wilson's disease

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/110871/1/hep27663.pd

Transplant livers in wilson's disease for hepatic, not neurologic, indications

No Abstract.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/35274/1/500060522_ftp.pd

DNA/protein complexes delivered in conjunction with adenovirus generate high-efficiency in vitro transfection: A powerful transfection reagent with potential broad applications

We have combined a receptor-mediated DNA delivery system with the endosomal lysis ability of adenovirus and shown that DNA can be delivered into primary hepatocytes, resulting in a high level of gene expression. When asialoorosomucoid conjugated with poly(L-lysine) was used to deliver the Escherichia coli Β-galactosidase gene into primary hepatocytes through binding with the hepatic asialoglycoprotein receptor, only a low level of Β-galactosidase was detectable, with less than 0.1% of the hepatocytes being transfected. This level of activity can be greatly enhanced by the cointernalization of the DNA protein complex with a replication-defective adenovirus, resulting in 100% of the hepatocytes staining blue with 5-bromo-4-chloro-3-indolyI Β-D-galactoside. Quantitative analysis of Β-galactosdase expression also showed a 1000-fold enhancement of activity. To test the applicability of this DNA delivery system for the correction of phenylketonuria, a metabolic disorder that causes severe mental retardation in children, we have delivered the human phenylalanine hydroxylase (PAH) gene to hepatocytes derived from a PAH-deficient mouse strain and dem-onstrated complete reconstitution of enzymatic activity. This method shows great promise for efficient gene delivery to the liver for correction of hepatic disorders.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/38404/1/1840180639_ftp.pd

Anticoagulation Therapy in Microsurgery: A Review

The advent of microsurgical tissue transfer including replantation greatly has expanded the scope of reconstructive surgery. There are few recent innovations in anticoagulation therapies for microsurgery, however, and anastomotic thrombosis remains an occasional cause of surgical failure. No consensus exists on the ideal anticoagulation protocol for microsurgery. This article reviews major pharmacologic modalities of anticoagulation, delineates the mechanism of action and study of efficacy of each agent, and compares the risks and benefits of popular anticoagulation therapies. Finally, it examines available human outcomes–based data and attempts to provide a glimpse of the future direction of microsurgical anticoagulation research