Antioxidant and antimicrobial activities with GC/MS analysis of the Morus alba L. Leaves

Morus alba L. Moraceae leaves are used effectively to treat fever, protect liver from damage, strengthen the joints, facilitate discharge of urine in Turkey folk medicine. In this study we aimed to determine in vitro antioxidant and antimicrobial activity with GC/MS analysis of the Morus alba L. leaves. Dried plant leaves samples are milled and extracted with distilled water and ethanol in Soxhlet machine. After extraction, extract samples were concentrated in rotary evaporator machine. Total antioxidant status values were determined as mmol Trolox Equivalent/L spectrophotometrically by using Erel’s method. Sterile extracts were used to avoid contamination in antimicrobial activity test. As test microorganisms Escherichia coli ATCC 25922 , Staphylococcus aureus ATCC 25923 , Enterococcus faecalis ATCC 29212 , Pseudomonas aeroginosa ATCC 27853 , Candida albicans ATCC 90028 were used. Antimicrobial activity was determined with disc diffusion method. We used ethanol extracts of samples in GC/MS analysis. Ethanol and distilled water extracts showed antioxidant activity in different rate. Besides, ethanol extracts have an antimicrobial activity but water extracts have not antimicrobial activity on our test microorganisms. We determined four compounds 9,12,15-octadecatrienoic acid, ethyl ester, linolenic acid ethyl ester, gibberellic acid with GC/MS analysis in ethanol extract

The Effect of Isotretinoin on Oocyte Maturation in Adolescent Female Rats

Objective: Isotretinoin is used in acne vulgaris treatment for more than 20 years. Isotretinoin has serious side effects on many organs, but there are no comprehensive studies investigating its possible toxic effects on reproductive organs. Thus, we aimed to investigate the possible toxic effects of isotretinoin administration on oocyte maturation in female rat gonads in this study. Methods: Thirty-two adolescent female rats (Wistar Albino, 220 ± 35 g) were randomly divided into 4 groups with 8 subjects in each group: group 1, group 2, group 3, and group 4. Different doses of isotretinoin which was dissolved in sesame oil were given to rats by gavage: 7.5 mg/kg/day in group 3 and 15 mg/kg/day in group 4. The rats in group 2 received sesame oil by gavage. To create gavage stress, only gavage was administered to the rats in group 1. The gavages for each group continued once a day and at a certain time for 30 days. To determine the effect of isotretinoin on oocyte maturation, the periodic acid-Schiff reaction was performed for histochemical and histomorphometric evaluation of the zona pellucida, and staining of growth differentiation factor-9 (GDF-9) and bone morphogenetic protein-15 (BMP-15) was performed for immunohistochemical analysis. Results: When the thickness of the zona pellucida was evaluated, a statistically significant difference was found between group 1 and experimental groups (group 3 and group 4). In the experimental groups, it was determined that the thickness of the zona pellucida was decreased depending on the increase in dose. GDF-9 and BMP-15 expressions in oocytes of primordial and primary follicles decreased significantly in the experimental groups compared to group 1 and group 2. However, the expression of GDF-9 and BMP-15 in oocytes of secondary follicles was not significantly different between group 1 and group 2 and the experimental groups. Conclusions: In our study, we showed toxic effect of isotretinoin on oocyte maturation in female rats. © 2020 S. Karger AG, Basel