Serum Activins and Follistatin during the Treatment of Chronic Hepatitis C Genotypes 1 and 4 and Their Correlations with Viral Load and Liver Enzymes: A Preliminary Report

Aims. To measure the effect of pegylated interferon-α therapy on serum activin-A, activin-B, and follistatin and their correlation with viral load and liver fibrosis in chronic hepatitis C (CHC). Methods. This study was cross-sectional and sera were collected from 165 participants classified into 7 groups: 40 healthy negative control, 33 treatment naïve patients as positive control, 19 patients at week 4, 22 at week 12, and 19 at week 24 of treatment initiation and 21 responders and 11 nonresponders at the end of 48-week treatment protocol. Serum candidate proteins were measured using ELISA and liver fibrosis was assessed by AST platelet ratio index (APRI). Results. CHC significantly increased activins and decreased follistatin compared to negative control (P<0.05). Activin-A and follistatin levels returned to the levels of negative control group at weeks 4, 12, and 24 following treatment initiation and were significantly different from positive control (P<0.05). Both proteins were significantly different between responders and nonresponders. Activin-A correlated positively and significantly with the viral load and APRI. Conclusion. CHC modulates serum activin-A and follistatin and they appear to be influenced by pegylated interferon-α therapy. Further studies are needed to explore the role of activins in CHC

Combinatorial strategies based on CRAd-IL24 and CRAd-ING4 virotherapy with anti-angiogenesis treatment for ovarian cancer

BACKGROUND: A major hurdle incurrent to the human clinical application of conditionally replicative adenovirus (CRAd)-based virotherapy agents is their limited therapeutic efficacy. In this study we evaluated whether arming our previously reported Ad5/3Δ24 CRAd vector containing a 24-base pair deletion in the E1A conserved region 2, which allows selective replication within Rb-p16-deficient tumor cells, to express therapeutic genes could improve oncolytic virus potency in ovarian cancer cells. We choose to assess the therapeutic benefits achieved by virus-mediated expression of interleukin 24 (IL-24), a cytokine-like protein of the IL-10 family, and the inhibitor of growth 4 (ING4) tumor suppressor protein. RESULTS: The generated CRAd-IL24 and CRAd-ING4 vectors were tested in ovarian cancer cell lines in vitro to compare their replication, yield, and cytotoxic effects with control CRAd Ad5/3∆24 lacking the therapeutic gene. These studies showed that CRAd-IL24 infection resulted in significantly increased yield of infectious particles, which translated to a marked enhancement of virus-induced cytotoxic effects as compared to CRAd-ING4 and non-armed CRAd. Testing CRAd-IL24 and CRAd-ING4 vectors combined together did not revealed synergistic effects exceeding oncolytic potency of single CRAD-IL24 vector. Both CRAds were also tested along with anti-VEGF monoclonal antibody Avastin and showed no significant augmentation of viral cytolysis by anti-angiogenesis treatment in vitro. CONCLUSIONS: Our studies validated that arming with these key immunomodulatory genes was not deleterious to virus-mediated oncolysis. These findings thus, warrant further preclinical studies of CRAd-IL24 tumoricidal efficacy in murine ovarian cancer models to establish its potential utility for the virotherapy of primary and advanced neoplastic diseases

Effectiveness of Food Safety and Hygiene Training Program for Hospital Food Services Staff in Holly Makkah

Foodborne diseases have been increasing in recent years, with a greater impact on the health and economy of developing countries. Food can be a potential source of infection and disease, right from the point of procurement to the point of consumption. It is more likely in the food service establishments where mass food is prepared. Hygienic practices at procuring, preparing and serving are essential. Food handlers specially play a major role here. The aim of this study was to assess the knowledge, attitudes and practices of food service staff regarding food safety and hygiene, before and after food safety education program in hospital. A cross-sectional design was used to assess the knowledge, attitudes and practices about food safety of the food services staff for the purpose of the study before and after a nutrition education program in a systemic sample of a hospital at holy Makkah were assessment by means of a questionnaire survey.After obtaining approval, the questionnaire was addressed to all food services staff. The study included 107 food handlers. Sixty of them (56.1%) were males and 47 were females (43.9%). Their age ranged between 22 and 56 years. Almost half of them (49%) were at least university graduated. Most of them (80.4%) were workers whereas (13.7%) were dieticians. Data analysis was carried out using the Statistical Package of the Social Science (SPSS) version 20. Overall mean percentage of knowledge before an educational intervention was 56.1% and after an intervention it became 77.7%. This difference was statistically significant, p&lt;0.001. It is concluded that the overall knowledge, attitudes and practices scores were higher regarding personnel hygiene, however, it need reconsideration regarding food-borne diseases and sanitation  Since the education, training of those handling food would improve the status of food hygiene knowledge. Keywords: Food Safety, Hygiene, Training Program, Hospital Food Services and Services Staff

Review Article Activins and Follistatin in Chronic Hepatitis C and Its Treatment with Pegylated-Interferon-Based Therapy

Pegylated-interferon-based therapy for the treatment of chronic hepatitis C (CHC) is considered suboptimal as not all patients respond to the treatment and it is associated with several side effects that could lead to dose reduction and/or termination of therapy. The currently used markers to monitor the response to treatment are based on viral kinetics and their performance in the prediction of treatment outcome is moderate and does not combine accuracy and their values have several limitations. Hence, the development of new sensitive and specific predictor markers could provide a useful tool for the clinicians and healthcare providers, especially in the new era of interferon-free therapy, for the classification of patients according to their response to the standard therapy and only subscribing the novel directly acting antiviral drugs to those who are anticipated not to respond to the conventional therapy and/or have absolute contraindications for its use. The importance of activins and follistatin in the regulation of immune system, liver biology, and pathology has recently emerged. This review appraises the up-to-date knowledge regarding the role of activins and follistatin in liver biology and immune system and their role in the pathophysiology of CHC

The prevalence of dengue virus serotypes in asymptomatic blood donors reveals the emergence of serotype 4 in Saudi Arabia

Abstract Background Transmission of dengue virus (DENV) through blood transfusion has been documented and hence screening for DENV during blood donation has been recently recommended by the American Association of Blood Banks and Centres of Disease Control and Prevention. DENV is endemic in the Western province of the Kingdom of Saudi Arabia (KSA) and serotypes 1, 2 and 3, but not 4, have been detected. However, little is known regarding the rates of DENV during blood donation in the kingdom. The aim of this study was therefore to measure the prevalence of dengue virus and its serotypes in eligible Saudi blood donors in the endemic Western region of KSA. Methods This was a cross-sectional study and serum samples were collected from 910 eligible Saudi male blood donors. DENV IgM and IgG antibodies were measured serologically by ELISA while viral serotypes were detected by a single step IVD CE certified multiplex RT-PCR kit. Results The overall prevalence was 39 and 5.5% for IgG+ and IgM+, respectively. There were 12 (1.3%) with exclusively IgM+, 317 (34.8%) exclusively IgG+ and 38 (4.2%) with dual IgM+/IgG+ donors. The overall prevalence was 3.2% (n = 29) and 2.3% (n = 21) for primary and secondary infections. PCR was positive in 5.5% (n = 50) and, DENV-2 (n = 24; 48%) was the most frequent serotype and was significantly higher than DENV-1 (20%; P = 0.02) and DENV-3 (2%; P = 0.1 × 10−5) but not DENV-4 (30%; P = 0.2). There was no significant difference between both DENV-4 and DENV-1 (P = 0.4). The combination of the PCR and serology findings showed that 22 (2.4%) and 28 (3.1%) donors had primary and secondary viremic infections, respectively. Conclusions The detected rates of DENV by PCR suggest a potential high risk of viral transmission by blood transfusion. To the best of our knowledge, this study is the first to report the detection of DENV-4 serotype in Saudi Arabia. More studies are required to measure the precise prevalence of DENV serotypes and their potential transmission rate during blood donation in the kingdom

Quality of Life in Patients with Oral Potentially Malignant Disorders: Oral Lichen Planus and Oral Epithelial Dysplasia

Background Oral potential malignant disorders (OPMDs) are lesions and conditions that increase the risk for malignant transformation. OPMDs have an impact on patients’ health status and Quality of life (QoL). The aim of our study is to evaluate and compare QoL in patients with oral lichen planus and oral epithelial dysplasia as a setting of clinical leukoplakia. Methods This is a cross-sectional study with a sample size of 100 patients divided into, 53 subjects with Oral Lichen Planus (53.0%), 39 patients with oral epithelial dysplasia as a setting of solitary leukoplakia (39.0%), and 8 subjects with oral lichen planus with oral epithelial dysplasia (8.0%). Patients who fit the criteria were asked to fill out three different questionnaires. 26-item Chronic Oral Mucosal Disease Questionnaire (COMDQ-26), Hospital Anxiety and Depression Scale (HADS), and the Oral potentially malignant disorders QoL questionnaire (OPMDsQoL). Results Both the COMD-26 and OPMDQoL questionnaire scores were significantly different across the three disease groups. Relative to patients aged \u3e65, the 40-64 age group added 6.8 points to the COMD-26 survey score (P \u3c 0.05). Relative to oral epithelial dysplasia, oral lichen planus added 15.7 points to the COMD-26 survey score (P \u3c 0.001). Relative to oral epithelial dysplasia, oral lichen plus added 8.9 points to the OPMDQoL survey score (P = 0.003). Conclusion Within the limitation of our study OLP shows significant poorer QoL in compared to OED as a setting of clinical OL. Younger individuals affected with OLP, and OED showed significant impact in QoL in compared to older individuals