A new robust diagnostic polymerase chain reaction for determining the mating status of female Anopheles gambiae mosquitoes.

The principal malaria vector in Africa, Anopheles gambiae, contains two pairs of autosomes and one pair of sex chromosomes. The Y chromosome is only associated with males and other Y chromosome-specific DNA sequences, which are transferred to women during mating. A reliable tool to determine the mating status of dried wild An. gambiae females is currently lacking. DNA was extracted from dried virgin and mated females and used to test whether Y chromosome-specific polymerase chain reaction (PCR) markers can be successfully amplified and used as a predictor of mating. Here we report a new PCR-based method to determine the mating status among successfully inseminated and virgin wild An. gambiae females, using three male-specific primers. This dissection-free method has the potential to facilitate studies of both population demographics and gene flow from dried mosquito samples routinely collected in epidemiologic monitoring and aid existing and new malaria-vector control approaches

Learning design in diverse institutional and cultural contexts: suggestions from a participatory workshop with higher education professionals in Africa

Learning design approaches, such as those adopted by the Open University, provide a set of tools and resources for purposefully-designing modules with a focus on student experiences. However, many of the current learning design strategies have been situated within specific institutions in Europe and North America. This means that there are several issues worth considering around if and how established learning design approaches make sense in diverse institutional and cultural contexts. To critically assess the relevance and appropriateness of learning design strategies in new contexts, this article describes an in-depth participatory workshop with 34 education professionals from five African countries. Altogether, 10 suggestions for learning design practices were derived from the consensus of workshop participants, which provide a foundation for the development of learning design practices moving forward

Conceptualizing Internationalization at a Distance: A "Third Category" of University Internationalization

Internationalization efforts in higher education have often been categorized according to Jane Knight’s binary of “Internationalization at Home” (IaH) and “Internationalization Abroad” (IA). However, a rising number of technology-supported activities have created new opportunities for university internationalization. For example, students can now remain “at home” while using technology to study with an institution or program that is simultaneously located “abroad.” We have conceptualized these activities as a new third category called Internationalization at a Distance (IaD). In this article, we introduce the concept of IaD and outline an in-depth case study of an international distance education provider at scale, the University of South Africa

Internationalisation at a Distance and at Home: Academic and social adjustment in a South African distance learning context

With the rise of technology and distance learning, a recently new type of internationalisation of higher education seems to be emerging in Southern Africa higher education, which we coin as Internationalisation at a Distance. In this empirical study, we aim to provide an initial attempt to theorise this form of Internationalisation at a Distance through an in-depth analysis of 1141 students’ experiences while studying at the largest distance learning institution in Southern Africa. Using an adjusted version of the Student Adaptation to College Questionnaire (SACQ) instrument developed by Baker and Siryk (1999), we have explored the study experiences of international students living at a distance, as well as South Africans and international students living in South Africa. Our regression models indicate that academic adjustment is significantly predicted by emotional adjustment, attachment towards the institution, access to technology, and internationalisation at home students. The results highlight the need for a much more complex narrative around internationalisation in distance learning settings in light of technological advances, requiring a potential reconsideration of what internationalisation ‘abroad’ and ‘at home’ might mean

Recovering from COVID-19 Building Closures: Guidance Document

While the country comes to terms with the inevitable impact that SARS-CoV-2, the virus that causes COVID-19, will have on our lives and communities, public health authorities remain focused on breaking the chain of transmission. Managing the risk has resulted in widespread closures of businesses, schools, universities, resorts, and other facilities deemed “non-essential.” Practically speaking, this means closing buildings and ceasing operations. For building owners and operators, this poses a significant challenge to protect their assets and to ensure they are ready to reoccupy once the pandemic subsides

Understanding the adjustment of first-year distance education students in South Africa: Factors that impact students’ experiences

Much research in face-to-face contexts outlines the importance of early adjustment on students’ higher education experiences. However, few studies have replicated this research in distance learning contexts to unpack the early multifaceted adjustments associated with studying in absence of a physical campus. This is particularly needed from a Global South perspective, where countries like South Africa have become regional hubs for distance learners. To explore distance learners’ adjustment experiences, this study analysed results from a Student Adaptation to College Questionnaire (SACQ) with 320 distance learners at the University of South Africa, mixed with qualitative thematic analysis of open-ended questions. The results outlined key factors that impact distance learning experiences for students in South Africa, including demographic variables, class, language, and access to resources. These findings, compared with similar work in face-to-face contexts, suggest areas in need of additional support from distance education providers in South Africa and beyond

Sickle cell patients are characterized by a reduced glycocalyx volume

The glycocalyx is an important anti-inflammatory and anti-adhesive barrier at the luminal side of endothelial cells. Glycocalyx volume was significantly reduced in sickle cell patients (HbSS/HbSβ0-thalassemia median 0.47L, IQR 0.27-0.66, HbSC/HbSβ+-thalassemia 0.23L, 0.0-0.58) compared with controls (1×109L, 0.52-1.77) (p=0.03). Reduced glycocalyx may be a new factor in the pathophysiology of sickle cell disease

Diverse metallicities of Fermi bubble clouds indicate dual origins in the disk and halo

The Galactic Center is surrounded by two giant plasma lobes known as the Fermi Bubbles, extending ~10 kpc both above and below the Galactic plane. Spectroscopic observations of Fermi Bubble directions at radio, ultraviolet, and optical wavelengths have detected multi-phase gas clouds thought to be embedded within the bubbles referred to as Fermi Bubble high-velocity clouds (FB HVCs). While these clouds have kinematics that can be modeled by a biconical nuclear wind launched from the Galactic center, their exact origin is unknown because, until now, there has been little information on their heavy-metal abundance (metallicity). Here we show that FB HVCs have a wide range of metallicities from <20% solar to ~320% solar. This result is based on the first metallicity survey of FB HVCs. These metallicities challenge the previously accepted tenet that all FB HVCs are launched from the Galactic center into the Fermi Bubbles with solar or super-solar metallicities. Instead, we suggest that FB HVCs originate in both the Milky Way's disk and halo. As such, some of these clouds may characterize circumgalactic medium that the Fermi Bubbles expand into, rather than material carried outward by the nuclear wind, changing the canonical picture of FB HVCs. More broadly, these results reveal that nuclear outflows from spiral galaxies can operate by sweeping up gas in their halos while simultaneously removing gas from their disks.Comment: This version of the article has been accepted for publication on Nature Astronomy after peer review. This version is not the Version of Record (https://doi.org/10.1038/s41550-022-01720-0) and does not reflect post-acceptance improvements, or any correction

The Grizzly, April 13, 2006

Relay a Success • New Healthcare Scholarship Available • Lurking Beneath the Skin: Scabies Infection • Day of Museums • Shopping with Eco-labels • Airband a Hit • Opinions: Drawing the Line: Moral Predicament of Abortion, Part II • Importance of CoSA: A Message • Bears Battle Back • Real Deal on Steroidshttps://digitalcommons.ursinus.edu/grizzlynews/1712/thumbnail.jp