Biological Control of F. Oxysporum F. Sp. Lycopersici Causing Wilt of Tomato by Pseudomonas Fluorescens

Abstract- Pseudomonas fluorescens is one of the major fungal biocontrol agents found in the soil and the rhizosphere of various crop systems. Ten isolates of P.fluorescens were isolated from rhizosphere soil samples collected from various tomato-growing fields and evaluated for their efficacy in increasing seed quality variables of tomato and in inhibiting the mycelial growth of Fusarium oxysporum. Pseudomonas isolate 2 produced effective results and was selected and mass multiplied. Talc and sodium alginate formulations of mass multiplied using different agents were prepared and evaluated for their effects against fusarium wilt under greenhouse conditions. Fresh cultures of Pf2 isolate was found to increase seedling emergence and reduce fusarium wilt disease incidence when compared to the control and the formulations

Selection of effective bio-​antagonistic bacteria for biological control of tomato wilt caused by Fusarium oxysporum F. sp. lycopersici

Bacteria from the rhizoplane soil and surrounding soil of healthy and Fusarium oxysporum diseased tomato plants of district regions of Karnataka were collected. The best bacterial strains, based on their ability to control development of Fusarium oxysporum isolate, were identified as BS1, BS5 and BS18. All bacterial isolates resulted effective for the in vitro control of growth of Fusarium oxysporum, where the control mechanisms used by the bacteria do not involve the secretion of fungal cell wall hydrolytic enzymes. On the other hand, all bacteria grew well in conditions similar to those that can be found at the field level (considering pH, salinity, Fe3+ and temp.) and showed a good capacity of tomato root colonization. These results suggest that Pseudomonas fluorescens isolates studied have an excellent potential to be used as biocontrol agents of Fusarium oxysporum in tomato greenhouses at the field level