Tumor-Specific Hsp70 Plasma Membrane Localization Is Enabled by the Glycosphingolipid Gb3

Human tumors differ from normal tissues in their capacity to present Hsp70, the major stress-inducible member of the HSP70 family, on their plasma membrane. Membrane Hsp70 has been found to serve as a prognostic indicator of overall patient survival in leukemia, lower rectal and non small cell lung carcinomas. Why tumors, but not normal cells, present Hsp70 on their cell surface and the impact of membrane Hsp70 on cancer progression remains to be elucidated.Although Hsp70 has been reported to be associated with cholesterol rich microdomains (CRMs), the partner in the plasma membrane with which Hsp70 interacts has yet to be identified. Herein, global lipid profiling demonstrates that Hsp70 membrane-positive tumors differ from their membrane-negative counterparts by containing significantly higher amounts of globotriaoslyceramide (Gb3), but not of other lipids such as lactosylceramide (LacCer), dodecasaccharideceramide (DoCer), galactosylceramide (GalCer), ceramide (Cer), or the ganglioside GM1. Apart from germinal center B cells, normal tissues are Gb3 membrane-negative. Co-localization of Hsp70 and Gb3 was selectively determined in Gb3 membrane-positive tumor cells, and these cells were also shown to bind soluble Hsp70-FITC protein from outside in a concentration-dependent manner. Given that the latter interaction can be blocked by a Gb3-specific antibody, and that the depletion of globotriaosides from tumors reduces the amount of membrane-bound Hsp70, we propose that Gb3 is a binding partner for Hsp70. The in vitro finding that Hsp70 predominantly binds to artificial liposomes containing Gb3 (PC/SM/Chol/Gb3, 17/45/33/5) confirms that Gb3 is an interaction partner for Hsp70.These data indicate that the presence of Gb3 enables anchorage of Hsp70 in the plasma membrane of tumors and thus they might explain tumor-specific membrane localization of Hsp70

Asea Brown Boveri, Deutschland. Geschaeftsbericht 1999

Available from TIB Hannover / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEDEGerman

Asea Brown Boveri, Mannheim. Geschaeftsbericht 1994

Es wird eine Beschreibung der Aufgaben und Aktivitaeten dieses international taetigen Grossunternehmens gegeben, wobei der Jahresabschluss 1994 zahlreiche finanzielle Daten (Bilanz, Gewinn- u. Verlustrechnung etc.) offenlegt. ABB ist ein weltweit taetiges Unternehmen der Elektro-, Verkehrs- und Umwelttechnik. Sitz der Konzernleitung ist Zuerich. Das Unternehmen entwickelt, plant, baut und wartet Produkte und Systeme auf den Gebieten Stromerzeugung, Stromuebertragung und Stromverteilung, Schienenverkehr sowie Industrie und Gebaeudetechnik. Hierzu zaehlen auch die Bereiche elektrisches Serienmaterial, Klimatisierung, Service, Allgemeiner Anlagenbau, Oel und Gas, Roboter, Turbolader und Leitungsbau. (orig./UA)The report describes the tasks and activities of this large international corporation and reveals numerous financial data (1994 annual balance sheet, profit-and-loss account, etc.). ABB is an enterprise in the electrical engineering, transport and environmental engineering sectors with subsidiaries in all parts of the world. The central management of the group is in Zuerich. It develops, plans, builds and maintains products and systems in the fields of power generation, power transmission and power distribution, railway transport as well as industrial and structural engineering. This includes also the sectors electrical series products, air-conditioning, service, construction of plants in general, oil and gas, robots, superchargers and construction of power transmission lines. (orig./UA)SIGLEAvailable from TIB Hannover / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDEGerman

Asea Brown Boveri Mannheim. Geschaeftsbericht 1991

SIGLEAvailable from FIZ Karlsruhe / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDEGerman

Racetrack-Spulen fuer hoehere Feldstaerken. Bd. 1 T. 1-8. Abschlussbericht

The aim of the present research project was the development and construction of training-poor superconducting racetrack coils for high field strengthes. The coils are planned for a magnetic system as part of a magnetic separator supplied with 4 superconducting coils placed into a helium cryostat. The report summarizes the coil design, the field calculation for the optimization of the magnetic separator, the calculation of the mechanical behaviour, the tools and devices as well as the manufacturing and the testing of the coils. The aim of the project was achieved by the construction and operational testing of the second racetrack coilSIGLEAvailable from TIB Hannover: F93B328+a / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Forschung und Technologie (BMFT), Bonn (Germany)DEGerman

Racetrack-Spulen fuer hoehere Feldstaerken. Bd. 2 T. 9 und 10. Abschlussbericht

The aim of the present research project was the development and construction of training-poor superconductig racetrack coils for high field strengthes. The coils are planned for a magnetic system as part of a magnetic separapor supplied with 4 superconducting coils placed into a helium cryostat. The present second volume of the report contains the technical drawings of the magnetic separator and of the tools and devicesSIGLEAvailable from TIB Hannover: F93B329+a / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Forschung und Technologie (BMFT), Bonn (Germany)DEGerman

Asea Brown Boveri, Mannheim. Geschaeftsbericht 1998

Available from TIB Hannover / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEDEGerman