16 research outputs found
Epidemiology of plasmid-mediated quinolone resistance in salmonella enterica serovar typhimurium isolates from food-producing animals in Japan
A total of 225 isolates of Salmonella enterica serovar Typhimurium from food-producing animals collected between 2003 and 2007 were examined for the prevalence of plasmid-mediated quinolone resistance (PMQR) determinants, namely qnrA, qnrB, qnrC, qnrD, qnrS, qepA and aac(6')Ib-cr, in Japan. Two isolates (0.8%) of S. Typhimurium DT104 from different dairy cows on a single farm in 2006 and 2007 were found to have qnrS1 on a plasmid of approximately 9.6-kbp. None of the S. Typhimurium isolates had qnrA, qnrB, qnrC, qnrD, qepA and acc(6')-Ib-cr. Currently in Japan, the prevalence of the PMQR genes among S. Typhimurium isolates from food animals may remain low or restricted. The PFGE profile of two S. Typhimurium DT104 isolates without qnrS1 on the farm in 2005 had an identical PFGE profile to those of two S. Typhimurium DT104 isolates with qnrS1. The PFGE analysis suggested that the already existing S. Typhimurium DT104 on the farm fortuitously acquired the qnrS1 plasmid
ヒト モウハツ ノ デンシ ジョウジセイ キョウメイ キュウシュウ キョウド : ヒト モウハツ ノ デンシ ジョウジセイ キョウメイ キュウシュウ キョウド ノ セイジョウ トクニ ホウシャセン ショウシャ コウカノ コタイ トクイセイ ニツイテ
Phylogenetic groups and cephalosporin resistance genes of Escherichia coli from diseased food-producing animals in Japan
A total of 318 Escherichia coli isolates obtained from different food-producing animals affected with colibacillosis between 2001 and 2006 were subjected to phylogenetic analysis: 72 bovine isolates, 89 poultry isolates and 157 porcine isolates. Overall, the phylogenetic group A was predominant in isolates from cattle (36/72, 50%) and pigs (101/157, 64.3%) whereas groups A (44/89, 49.4%) and D (40/89, 44.9%) were predominant in isolates from poultry. In addition, group B2 was not found among diseased food-producing animals except for a poultry isolate. Thus, the phylogenetic group distribution of E. coli from diseased animals was different by animal species. Among the 318 isolates, cefazolin resistance (minimum inhibitory concentrations: ≥32 μg/ml) was found in six bovine isolates, 29 poultry isolates and three porcine isolates. Of them, 11 isolates (nine from poultry and two from cattle) produced extended spectrum β-lactamase (ESBL). The two bovine isolates produced blaCTX-M-2, while the nine poultry isolates produced blaCTX-M-25 (4), blaSHV-2 (3), blaCTX-M-15 (1) and blaCTX-M-2 (1). Thus, our results showed that several types of ESBL were identified and three types of β-lactamase (SHV-2, CTX-M-25 and CTX-M-15) were observed for the first time in E. coli from diseased animals in Japan
Is water at farms a source of Campylobacter spp. contamination in live chickens in Khon Kaen Province of Thailand?
This study is the first to quantify the Campylobacter contamination in chicken farm waters. Water samples from 10
broiler chicken farms in Khon Kaen, Thailand, were collected during winter, summer, and rainy season. Four types of water i.e.
3 drinking water, 1 main tank water, 1 evaporative pond water, and 1 environmental water sample were collected at each farm
twice during 1-15, and 16-35 days of rearing. In total, 360 water samples were sampled. The overall occurrence rate of contamination
was 0.3% (1/360). Only 1 environmental water sample (1.7%, 1/60) was found contaminated with Campylobacter
species. Water collected during the later stage of rearing (16-35 days) was positive for Campylobacter spp. contamination.
The extent of contamination was greater than 230 MPN/100 ml. However, drinking and main tank water were free of
Campylobacter spp. contamination