Eddies in the Western Arctic Ocean From Spaceborne SAR Observations Over Open Ocean and Marginal Ice Zones

The Western Arctic Ocean is a host to major ocean circulation systems, many of which generate eddies that can transport water masses and corresponding tracers over long distances from their formation sites. However, comprehensive observations of critical eddy characteristics are currently not available and are limited to spatially and temporally sparse in situ observations. Here we use high‐resolution spaceborne synthetic aperture radar measurements to detect eddies from their surface imprints in ice‐free sea surface roughness, and in sea ice patterns throughout marginal ice zones. We provide the first estimate of eddy characteristics extending over the seasonally ice‐free and marginal ice zone regions of the Western Arctic Ocean, including their locations, diameters, and monthly distribution. Using available synthetic aperture radar data, we identified over 4,000 open ocean eddies, as well as over 3,500 eddies in marginal ice zones from June to October in 2007, 2011, and 2016. Eddies range in size between 0.5 and 100 km and are frequently found over the shelf and near continental slopes but also present in the deep Canada Basin and over the Chukchi Plateau. We find that cyclonic eddies are twice more frequent compared to anticyclonic eddies at the surface, distinct from the dominating anticyclonic eddies observed at depth by in situ moorings and ice‐tethered profilers. Our study supports the notion that eddies are ubiquitous in the Western Arctic Ocean even in the presence of sea ice and emphasizes the need for improved ocean observations and modeling at eddy scales

Eddies in the Western Arctic Ocean From Spaceborne SAR Observations Over Open Ocean and Marginal Ice Zones

The Western Arctic Ocean is a host to major ocean circulation systems, many of which generate eddies that can transport water masses and corresponding tracers over long distances from their formation sites. However, comprehensive observations of critical eddy characteristics are currently not available and are limited to spatially and temporally sparse in situ observations. Here we use high‐resolution spaceborne synthetic aperture radar measurements to detect eddies from their surface imprints in ice‐free sea surface roughness, and in sea ice patterns throughout marginal ice zones. We provide the first estimate of eddy characteristics extending over the seasonally ice‐free and marginal ice zone regions of the Western Arctic Ocean, including their locations, diameters, and monthly distribution. Using available synthetic aperture radar data, we identified over 4,000 open ocean eddies, as well as over 3,500 eddies in marginal ice zones from June to October in 2007, 2011, and 2016. Eddies range in size between 0.5 and 100 km and are frequently found over the shelf and near continental slopes but also present in the deep Canada Basin and over the Chukchi Plateau. We find that cyclonic eddies are twice more frequent compared to anticyclonic eddies at the surface, distinct from the dominating anticyclonic eddies observed at depth by in situ moorings and ice‐tethered profilers. Our study supports the notion that eddies are ubiquitous in the Western Arctic Ocean even in the presence of sea ice and emphasizes the need for improved ocean observations and modeling at eddy scales

Biomanufacturing and testbed development for the continuous production of monoclonal antibodies

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Palm Fruit Bioactives augment expression of Tyrosine Hydroxylase in the Nile Grass Rat basal ganglia and alter the colonic microbiome

Tyrosine hydroxylase (TH) catalyzes the hydroxylation of L-tyrosine to L-DOPA. This is the rate-limiting step in the biosynthesis of the catecholamines - dopamine (DA), norepinephrine (NE), and epinephrine (EP). Catecholamines (CA) play a key role as neurotransmitters and hormones. Aberrant levels of CA are associated with multiple medical conditions, including Parkinson's disease. Palm Fruit Bioactives (PFB) significantly increased the levels of tyrosine hydroxylase in the brain of the Nile Grass rat (NGR), a novel and potentially significant finding, unique to PFB among known botanical sources. Increases were most pronounced in the basal ganglia, including the caudate-putamen, striatum and substantia nigra. The NGR represents an animal model of diet-induced Type 2 Diabetes Mellitus (T2DM), exhibiting hyperglycemia, hyperinsulinemia, and insulin resistance associated with hyperphagia and accelerated postweaning weight gain induced by a high-carbohydrate diet (hiCHO). The PFB-induced increase of TH in the basal ganglia of the NGR was documented by immuno-histochemical staining (IHC). This increase in TH occurred equally in both diabetes-susceptible and diabetes-resistant NGR fed a hiCHO. PFB also stimulated growth of the colon microbiota evidenced by an increase in cecal weight and altered microbiome.  The metabolites of colon microbiota, e.g. short-chain fatty acids, may influence the brain and behavior significantly

Palm Fruit Bioactives modulate human astrocyte activity in vitro altering the cytokine secretome reducing levels of TNFα, RANTES and IP-10

Neurodegenerative diseases, including Alzheimer’s disease and Parkinson’s disease, are becoming more prevalent and an increasing burden on society. Neurodegenerative diseases often arise in the milieu of neuro-inflammation of the brain. Reactive astrocytes are key regulators in the development of neuro-inflammation. This study describes the effects of Palm Fruit Bioactives (PFB) on the behavior of human astrocytes which have been activated by IL-1β. When activated, the astrocytes proliferate, release numerous cytokines/chemokines including TNFα, RANTES (CCL5), IP-10 (CXCL10), generate reactive oxygen species (ROS), and express specific cell surface biomarkers such as the Intercellular Adhesion Molecule (ICAM), Vascular Cellular Adhesion Molecule (VCAM) and the Neuronal Cellular Adhesion Molecule (NCAM). Interleukin 1-beta (IL-1β) causes activation of human astrocytes with marked upregulation of pro-inflammatory genes. We show significant inhibition of these pro-inflammatory processes when IL-1β-activated astrocytes are exposed to PFB. PFB causes a dose-dependent and time-dependent reduction in specific cytokines: TNFα, RANTES, and IP-10. We also show that PFB significantly reduces ROS production by IL-1β-activated astrocytes. Furthermore, PFB also reduces the expression of ICAM and VCAM, both in activated and naïve human astrocytes in vitro. Since reactive astrocytes play an essential role in the neuroinflammatory state preceding neurodegenerative diseases, this study suggests that PFB may have a potential role in their prevention and/or treatment

Coordination to Imidazole Ring Switches on Phosphorescence of Platinum Cyclometalated Complexes: The Route to Selective Labeling of Peptides and Proteins via Histidine Residues

In this study, we have shown that substitution of chloride ligand for imidazole (Im) ring in the cyclometalated platinum complex Pt­(phpy)­(PPh₃)Cl (<b>1</b>; phpy, 2-phenylpyridine; PPh₃, triphenylphosphine), which is nonemissive in solution, switches on phosphorescence of the resulting compound. Crystallographic and nuclear magnetic resonance (NMR) spectroscopic studies of the substitution product showed that the luminescence ignition is a result of Im coordination to give the [Pt­(phpy)­(Im)­(PPh₃)]Cl complex. The other imidazole-containing biomolecules, such as histidine and histidine-containing peptides and proteins, also trigger luminescence of the substitution products. The complex <b>1</b> proved to be highly selective toward the imidazole ring coordination that allows site-specific labeling of peptides and proteins with <b>1</b> using the route, which is orthogonal to the common bioconjugation schemes via lysine, aspartic and glutamic acids, or cysteine and does not require any preliminary modification of a biomolecule. The utility of this approach was demonstrated on (i) site-specific modification of the ubiquitin, a small protein that contains only one His residue in its sequence, and (ii) preparation of nonaggregated HSA-based Pt phosphorescent probe. The latter particles easily internalize into the live HeLa cells and display a high potential for live-cell phosphorescence lifetime imaging (PLIM) as well as for advanced correlation PLIM and FLIM experiments