Development of Functional Models for a SOD

Superoxide dismutase (SOD) is the scavenger of superoxide anion (O2−) and functions as a protector of living bodies. Study of a model compound of SOD is important when searching for the relationship between functions and structures of enzymes. Furthermore, SOD model compounds have potential for therapeutic usefulness. Although many SOD: model compounds have been reported, their structures are quite different from those of the native enzyme. Cu,Zn-SOD has been proposed for clinical uses. Unfortunately, many problems such as half-lifetime and antigenicity have not been overcome even though several copper(II) complexes are known to show SOD activity. Active oxygen species such as superoxide (O2−) from various components of the cellular electron transport chains, and provided during the respiratory burst of phagocytic cells, have been implicated both in the aging process and in degenerative diseases, including arthritis and cancer. Therefore, the biological system posseses the protective mechanisms against active species

Genomic alterations in low-grade, anaplastic astrocytomas and glioblastomas.

RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are.To extend our understanding of potential stepwise genetic alterations that may underlie tumor progression from low-grade astrocytomas to glioblastomas, histopathologic and comparative genomic hybridization analyses were performed on tumor specimens from 68 primary lesions, including 40 glioblastomas, 10 anaplastic and 18 low-grade astrocytomas. The number of aberrations per case increased towards the higher grade tumors (grade II: 1.66+/-1.49; grade III: 2.80+/-1.68; grade IV: 3.02+/-1.07; F=6.955, p=0.002). A gain of 7/7q was common and the most frequently seen aberration in low-grade astrocytomas, whereas loss of 10q was the most frequently seen anomaly in anaplastic astrocytomas and glioblastomas. Chromosome 7p amplification was only detected in glioblastomas. Chromosome 10/10q deletion and combination of 1p, 19q and 17p deletions were specific to high-grade astrocytic tumors. Sequences of chromosome 7 and 10 seem to have pivotal roles in the biology of human gliomas. The genomic copy deletions of chromosomes 1p and 19q might provide an alternative mechanism in the genesis of astrocytomas

An Investigation upon DNA Interaction of 2 3 , 9 10 , 16 17 , 23 24 –Tetrakis 4- 4- 2-phenylprop-2-yl phenoxy Phthalocyanine Compound

The DNA binding activity of previously synthesized and characterized 2 3 , 9 10 , 16 17 , 23 24 –Tetrakis 4- 4- 2-phenylprop-2-yl phenoxy phthalocyanine compound PcF with CT-DNA was studied using UV/Vis, emission spectroscopic titrations, the melting temperature, viscosity measurement, and agarose gel electrophoresis methods in a Tris-HCl buffer solution at a pH of 7.1 at room temperature. The absorption titration spectra studies of PcF showed that absorbance intensities were decreased with increasing of concentrations of CT-DNA and the finding proved that the compound interacts with the DNA. Addition to absorption titration study, emission spectroscopic titration, the melting temperature, viscosity measurement, and agarose gel electrophoresis methods were also performed to investigate the binding activities of PcF with the DNA. The results of these methods confirmed the findings of absorption spectra study that the compound interacts with the DNA

DNA-Binding Study of Tetraaqua-bis(p-nitrobenzoato)cobalt(II) Dihydrate Complex: [Co(H2O)4(p-NO2C6H4COO)2]·2H2O

The interaction of [Co(H2O)4(p-NO2C6H4COO)2]. 2H2O with sheep genomicDNA has been investigated by spectroscopic studies and electrophoresis measurements.The interaction between cobalt(II) p-nitrobenzoate and DNA has been followed by gelelectrophoresis while the concentration of the complex was increased from 0 to 14 mM.The spectroscopic study and electrophoretic experiments support the fact that the complexbinds to DNA by intercalation via p-nitrobenzoate into the base pairs of DNA. Themobility of the bands decreased as the concentration of complex was increased, indicatingthat there was increase in interaction between the metal ion and DNA

Study of Polymorph Prediction for L-Ascorbic Acid [Int. J. Mol. Sci. 2005, 6, 291-302]

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Studies on the Interaction between Zinc-Hydroxybenzoite Complex and Genomic DNA

Zinc-Hydroxybenzoite ([Zn (H20)6] (p-HO-C6H4COO)22H20) complex which wassynthesized and characterized by instrumental methods and the DNA samples which hadbeen isolated from cattle were allowed to interact at 37 oC for different time periods. Theinteraction of genomic DNA with this complex has been followed by agarose gelelectrophoresis at 50 V for 2 h. When DNA samples were allowed to interact with this metalcomplex, it was found that band intensities changed with the concentrations of the complex.In the result of interaction between this complex and genomic DNA samples, it wasdetermined that the intensities of bands were changed at the different concentrations of thecomplex. The brightness of the bands was increased and mobility of the bands wasdecreased, indicating the occurrence of increased covalent binding of the metal complexwith DNA. In this study it was concluded that the damage effect of ascorbate was reducedby Zinc-Hydroxybenzoite

The Interaction of Sheep Genomic DNA with a Cobalt(II) Complex Containing p-Nitrobenzoate and N,N`-Diethylnicotinamide Ligands

The synthesized cobalt(II) complex, CoPNBDENA and the binding of thiscomplex with sheep genomic DNA were investigated by UV–Visible absorption andviscosity techniques. Also the interaction of sheep genomic DNA with the complex wasstudied using the agarose gel electrophoresis method. The results indicated that thecomplex interacted with DNA. The nature of the binding seemed to be mainly anelectrostatic interaction between DNA and the cobalt(II) complex. Other binding modessuch as hydrogen bonds may also exist in this system. In this study, after the interaction ofDNA– CoPNBDENA, it was observed that the migration of the DNA band became slow asthe amount of cobalt(II) complex was increased. This clearly demonstrates that theCoPNBDENA complex neutralizes the negative charges of DNA

DNA-Binding Properties of Nickle II Phthalocyanine with 2-Isopropyl-5-Methylphenoxy Substituents and Their Studies of UV/Vis, Fluorescence Spectroscopies, Cyclic voltametry, Gel Eelectrophoresis and Viscosity Measurement

I n this study, previously synthesized Ni II phthalocyanine compound bearing 2,10,16,24-tetrakis 2-isopropyl-5-methylphenoxy group was chosen for its interaction with calf thymus-DNA. Calf thymus-DNA was used to determine DNA binding properties of Ni II phthalocyanine compound. The DNA binding activities of Ni II phthalocyanine compound bearing 2-isopropyl-5-methylphenoxy substituent was investigated by using absorption titration, fluorescence emission, cyclic voltammetry, gel elctrophoresis in Tris-HCl buffer at pH 7.0. In addition to above methods, melting point and viscosity experiment were performed to determine the DNA intercation of the compound in Tris-HCl buffer solution. The results showed that Ni II phthalocyanine compound binds strongly to calf thymus-DNA via intercalation bindin

DNA-Binding Study of Tetraaqua-bis(p-nitrobenzoato)cobalt(II) Dihydrate Complex: [Co(H2O)4(p-NO2C6H4COO)2]·2H2O

The interaction of [Co(H2O)4(p-NO2C6H4COO)2]. 2H2O with sheep genomicDNA has been investigated by spectroscopic studies and electrophoresis measurements.The interaction between cobalt(II) p-nitrobenzoate and DNA has been followed by gelelectrophoresis while the concentration of the complex was increased from 0 to 14 mM.The spectroscopic study and electrophoretic experiments support the fact that the complexbinds to DNA by intercalation via p-nitrobenzoate into the base pairs of DNA. Themobility of the bands decreased as the concentration of complex was increased, indicatingthat there was increase in interaction between the metal ion and DNA