The Secreted Acid Phosphatase Domain-Containing GRA44 from Toxoplasma gondii Is Required for c-Myc Induction in Infected Cells.

During host cell invasion, the eukaryotic pathogen Toxoplasma gondii forms a parasitophorous vacuole to safely reside within the cell, while it is partitioned from host cell defense mechanisms. From within this safe niche, parasites sabotage multiple host cell systems, including gene expression, apoptosis, and intracellular immune recognition, by secreting a large arsenal of effector proteins. Many parasite proteins studied for active host cell manipulative interactions have been kinases. The translocation of effectors from the parasitophorous vacuole into the host cell is mediated by a putative translocon complex, which includes the proteins MYR1, MYR2, and MYR3. Whether other proteins are involved in the structure or regulation of this putative translocon is not known. We have discovered that the secreted protein GRA44, which contains a putative acid phosphatase domain, interacts with members of this complex and is required for host cell effects downstream of effector secretion. We have determined that GRA44 is processed in a region with homology to sequences targeted by protozoan proteases of the secretory pathway and that both major cleavage fragments are secreted into the parasitophorous vacuole. Immunoprecipitation experiments showed that GRA44 interacts with a large number of secreted proteins, including MYR1. Importantly, conditional knockdown of GRA44 resulted in a lack of host cell c-Myc upregulation, which mimics the phenotype seen when members of the translocon complex are genetically disrupted. Thus, the putative acid phosphatase GRA44 is crucial for host cell alterations during Toxoplasma infection and is associated with the translocon complex which Toxoplasma relies upon for success as an intracellular pathogen.IMPORTANCE Approximately one-third of humans are infected with the parasite Toxoplasma gondii Toxoplasma infections can lead to severe disease in those with a compromised or suppressed immune system. Additionally, infections during pregnancy present a significant health risk to the developing fetus. Drugs that target this parasite are limited, have significant side effects, and do not target all disease stages. Thus, a thorough understanding of how the parasite propagates within a host is critical in the discovery of novel therapeutic targets. Toxoplasma replication requires that it enter the cells of the infected organism. In order to survive the environment inside a cell, Toxoplasma secretes a large repertoire of proteins, which hijack a number of important cellular functions. How these Toxoplasma proteins move from the parasite into the host cell is not well understood. Our work shows that the putative phosphatase GRA44 is part of a protein complex responsible for this process

Transversality of the logarithmic divergences in the Classical Finite Temperature SU(N) Self-Energy

We show that the logarithmic divergences that appear in the classical approximation of the finite temperature SU(N) self-energy are transverse. We use the Ward identities in linear gauges and the fact that the superficial degree of divergence d of a classical diagram only depends on the number of loops l via d=2-l. We comment on the relevance of this result to the construction of a low-energy effective theory beyond HTLs.Comment: 5 pages, 1 figure, REVTE

Distinguished self-adjoint extensions of Dirac operators via Hardy-Dirac inequalities

We prove some Hardy-Dirac inequalities with two different weights including measure valued and Coulombic ones. Those inequalities are used to construct distinguished self-adjoint extensions of Dirac operators for a class of diagonal potentials related to the weights in the above mentioned inequalities.Comment: 16 page

Ward Identities for the 2PI effective action in QED

We study the issue of symmetries and associated Ward-like identities in the context of two-particle-irreducible (2PI) functional techniques for abelian gauge theories. In the 2PI framework, the $n$-point proper vertices of the theory can be obtained in various different ways which, although equivalent in the exact theory, differ in general at finite approximation order. We derive generalized (2PI) Ward identities for these various $n$-point functions and show that such identities are exactly satisfied at any approximation order in 2PI QED. In particular, we show that 2PI-resummed vertex functions, i.e. field-derivatives of the so-called 2PI-resummed effective action, exactly satisfy standard Ward identities. We identify another set of $n$-point functions in the 2PI framework which exactly satisfy the standard Ward identities at any approximation order. These are obtained as field-derivatives of the two-point function \bcG^{-1}[\phi], which defines the extremum of the 2PI effective action. We point out that the latter is not constrained by the underlying symmetry. As a consequence, the well-known fact that the corresponding gauge-field polarization tensor is not transverse in momentum space for generic approximations does not constitute a violation of (2PI) Ward identities. More generally, our analysis demonstrates that approximation schemes based on 2PI functional techniques respect all the Ward identities associated with the underlying abelian gauge symmetry. Our results apply to arbitrary linearly realized global symmetries as well.Comment: 33 pages, 2 figure

Asymmetric Chern-Simons number diffusion from CP-violation

We study Chern-Simons number diffusion in a SU(2)-Higgs model with CP-odd dimension-eight operators. We find that the thermal average of the magnitude of the velocity of the Chern-Simons number depends on the direction of the velocity. This implies that the distribution function of the Chern-Simons number will develop an asymmetry. It is argued that this asymmetry manifests itself through a linear growth of the expectation value of the third power of the Chern-Simons number. This linear behavior of the third power of a coordinate of a periodic direction is verified by a numerical solution of a one-dimensional Langevin equation. Further, we make some general remarks on thermal averages and on the possibility of the generation of the baryon asymmetry in a non-equilibrium situation due to asymmetric diffusion of the Chern-Simons number

Non-equilibrium dynamics of a Bose-Einstein condensate in an optical lattice

The dynamical evolution of a Bose-Einstein condensate trapped in a one-dimensional lattice potential is investigated theoretically in the framework of the Bose-Hubbard model. The emphasis is set on the far-from-equilibrium evolution in a case where the gas is strongly interacting. This is realized by an appropriate choice of the parameters in the Hamiltonian, and by starting with an initial state, where one lattice well contains a Bose-Einstein condensate while all other wells are empty. Oscillations of the condensate as well as non-condensate fractions of the gas between the different sites of the lattice are found to be damped as a consequence of the collisional interactions between the atoms. Functional integral techniques involving self-consistently determined mean fields as well as two-point correlation functions are used to derive the two-particle-irreducible (2PI) effective action. The action is expanded in inverse powers of the number of field components N, and the dynamic equations are derived from it to next-to-leading order in this expansion. This approach reaches considerably beyond the Hartree-Fock-Bogoliubov mean-field theory, and its results are compared to the exact quantum dynamics obtained by A.M. Rey et al., Phys. Rev. A 69, 033610 (2004) for small atom numbers.Comment: 9 pages RevTeX, 3 figure

Parameter free Hubble constant from the quadruply lensed quasar SDSS J1004 + 4112

We present a free-form lens model for the multiply lensed quasar in the galaxy cluster SDSS J$1004+4112$. Our lens model makes minimal assumptions about the distribution of mass in the lens plane. We pay particular attention to the model uncertainties on the predicted time delay, originating from the particular configuration of model variables. Taking into account this uncertainty, we obtain a value of the Hubble constant of $H_0= 74^{+9}_{-13}$km s$^{-1}$ Mpc$^{-1}$, consistent with independent recent estimates. The predicted time delay between the central image E and image C (the first to arrive), is $\Delta T_{E-C}=3200\pm 200$ days. Future measurements of $\Delta T_{E-C}$ will allow to impose a tighter constrain on $H_0$ from this cluster-QSO system.Comment: 5 pages, 5 figure

2PI Effective Action and Evolution Equations of N = 4 super Yang-Mills

We employ nPI effective action techniques to study N = 4 super Yang-Mills, and write down the 2PI effective action of the theory. We also supply the evolution equations of two-point correlators within the theory.Comment: 16 pages, 6 figures. Figure 2 replaced, approximation scheme clarified, references adde

Equilibration in phi^4 theory in 3+1 dimensions

The process of equilibration in phi^4 theory is investigated for a homogeneous system in 3+1 dimensions and a variety of out-of-equilibrium initial conditions, both in the symmetric and broken phase, by means of the 2PI effective action. Two Phi-derivable approximations including scattering effects are used: the two-loop and the ``basketball'', the latter corresponding to the truncation of the 2PI effective action at O(lambda^2). The approach to equilibrium, as well as the kinetic and chemical equilibration is investigated.Comment: 32 pages, 14 figures, uses axodraw, minor corrections adde

Looking for defects in the 2PI correlator

Truncations of the 2PI effective action are seen as a promising way of studying non-equilibrium dynamics in quantum field theories. We probe their applicability in the non-perturbative setting of topological defect formation in a symmetry-breaking phase transition, by comparing full classical lattice field simulations and the 2PI formulation for classical fields in an O($N$) symmetric scalar field theory. At next-to-leading order in 1/N, the 2PI formalism fails to reproduce any signals of defects in the two-point function. This suggests that one should be careful when applying the 2PI formalism for symmetry breaking phase transitions.Comment: 22 pages, 6 figure