P5A-Type ATPase Cta4p Is Essential for Ca2+ Transport in the Endoplasmic Reticulum of Schizosaccharomyces pombe

This study establishes the role of P5A-type Cta4 ATPase in Ca2+ sequestration in the endoplasmic reticulum by detecting an ATP-dependent, vanadate-sensitive and FCCP insensitive 45Ca2+-transport in fission yeast membranes isolated by cellular fractionation. Specifically, the Ca2+-ATPase transport activity was decreased in ER membranes isolated from cells lacking a cta4+ gene. Furthermore, a disruption of cta4+ resulted in 6-fold increase of intracellular Ca2+ levels, sensitivity towards accumulation of misfolded proteins in ER and ER stress, stimulation of the calcineurin phosphatase activity and vacuolar Ca2+ pumping. These data provide compelling biochemical evidence for a P5A-type Cta4 ATPase as an essential component of Ca2+ transport system and signaling network which regulate, in conjunction with calcineurin, the ER functionality in fission yeast

Antimicrobial Resistance and Infection Control

Introduction Training Infection Control in Europe (TRICE) in 2010 identified significant differences within European Countries (EC) in the existence of Infection Control /Hospital Hygiene (IC/HH) courses and their compliance with the Improving Patient Safety in Europe (IPSE, 2008) recommended Core Competencies. The need to improve official recognition of “IC/HH degrees” for healthcare professionals also emerged. TRICE further developed, agreed EIC/HHCC with two tiers, published by ECDC in March 2013 as a Technical Document. Objectives Within the ECDC 2012 commissioned project TRICE-IS (Implementation Strategy) we conducted a comparative analysis between EIC/HHCC and IC/HH related disciplines within the broader aim of promoting mutual recognition of courses based on EIC/HHCC within European Countries. Methods We collected documents about disciplines that in TRICE were related to IC/HH (Medical Microbiology, Infectious Diseases, Public Health and Epidemiology) and compared them with European IC/HH Core Competencies. Results Documents collected were referred to:Public Health (ASPHER), Epidemiology (ECDC) Public Health Microbiology (EUPHEM, ECDC), Medical Microbiology (UEMS) and Infectious Diseases (UEMS). Global alignment with the EIC/HHCC competencies (n.101) resulted: 80% for ASPHER, 56% for epidemiology ECDC, 40% for Infectious Diseases, 20% Medical Microbiology UEMS and 64,4% for EUPHEM. In the Program Management area (n. 24 competencies) poor alignment has been identified for Infectious Diseases 37.5%,and Medical Microbiology (UEMS) 16.7% and Public Health Microbiology (EUPHEM) 4%. Conclusion European documents addressing the training of specialties related to IC/HH have many topics similar to those reported in the EIC/HHCC. Almost all of them need to be complemented in order to cover the topics mentioned in EIC/HHCC