Effect of Annonaceous Sp. Ethanolic Extract on Transforming Growth Factor- Β (TGF- Β) Expression on MCF-7 Cells Line

Aims: To analyze effect of the Annonaceous sp. ethanolic extract to Transforming Growth Factor- β (TGF- β) expressions on MCF-7 cells line. Methods: This experimental analytic study used Randomized Complete Block Design (RCBD) with three repetitions. The extract was dissolved in 1 ml of DMSO with a concentration of 0.1%. Furthermore, dilution was made with a dose of 0 µg / ml (K); 25 µg / ml (P1); 50 µg / ml (P2); 100 µg / ml (P3); 200 µg / ml (P4). MCF-7 cells line were cultured using RPMI 1640 Medium with 80 – 90% confluent. The ethanolic extract of Annonaceous sp. was exposed to MCF-7 cells for 48 hours. Analyze Transforming Growth Factor- β (TGF- β) level using ELISA Methods with λ=405 nm. Results: The results of the ELISA analysis show that the ethanolic extract of Annonaceous sp. has a potential effect of decreasing TGF- β expression on the sample with treatment on some concentration (p< 0,05). Conclusion: The ethanolic extract of Annonaceous sp. has shown the potential effect to decreased of TGF- β level on MCF-7 cells line Keywords: Annonaceous sp ethanolic extract, TGF- β, MCF-7 cells line, anti-cance

The Ethanolic Extract of Annonaceous sp. to Inhibit Vascular Endothelial Growth Factor (VEGF) as Anticancer Modalities

Introduction: Cancer has become a major public health problem worldwide, with breast cancer is the most common cancer among women and one of the most important causes of death among them. Management of breast cancer have some adverse effects, with many factorx=s shiouln be considered to ordered the therapy. Use of chemotherapy will suppress cell proliferation and trigger apoptosis, but it cannot just focus on cancer cells only, but also would affected on other cells and cause. Objective: This study aim to analyze the inhibition effect of the Annonanceous sp. ethanolic extract to Vascular Endothelial Growth Factor (VEGF) on MCF-7 cells line Material and Methods: This study is an analytic experimental study used Randomized Complete Block Design (RCBD) with three repetitions. The extract was dissolved in 1 ml of DMSO with a concentration of 0.1%. Furthermore, dilution was made with a dose of 0 µg / ml (K); 25 µg / ml (P1); 50 µg / ml (P2); 100 µg / ml (P3); 200 µg / ml (P4). MCF-7 cells line were cultured used RPMI 1640 Medium with 80 – 90% confluent. The ethanolic extract of Annonaceous sp. were exposed to MCF-7 cells for 48 hours. Analyze of VEGF level use ELISA Methods with λ=405 nm. Results: The results of ELISA analyze shows that the ethanolic extract of Annonaceous sp. have potential effect to decreased of VEGF expression on sample with treatment on some concentration (p< 0,05). Conclusion: The ethanolic extract of Annonaceous sp. has shows the potential effect to decreased of VEGF level on MCF-7 cells lin