Industrial Melanism in the Peppered Moth Is Not Associated with Genetic Variation in Canonical Melanisation Gene Candidates

Industrial melanism in the peppered moth (Biston betularia) is an iconic case study of ecological genetics but the molecular identity of the gene determining the difference between the typical and melanic (carbonaria) morphs is entirely unknown. We applied the candidate gene approach to look for associations between genetic polymorphisms within sixteen a priori melanisation gene candidates and the carbonaria morph. The genes were isolated and sequence characterised in B. betularia using degenerate PCR and from whole-transcriptome sequence. The list of candidates contains all the genes previously implicated in melanisation pattern differences in other insects, including aaNAT, DOPA-decarboxylase, ebony, tan, tyrosine hydroxylase, yellow and yellow2 (yellow-fa). Co-segregation of candidate gene alleles and carbonaria morph was tested in 73 offspring of a carbonaria male-typical female backcross. Surprisingly, none of the sixteen candidate genes was in close linkage with the locus controlling the carbonaria-typical polymorphism. Our study demonstrates that the ‘carbonaria gene’ is not a structural variant of a canonical melanisation pathway gene, neither is it a cis-regulatory element of these enzyme-coding genes. The implication is either that we have failed to characterize an unknown enzyme-coding gene in the melanisation pathway, or more likely, that the ‘carbonaria gene’ is a higher level trans-acting factor which regulates the spatial expression of one or more of the melanisation candidates in this study to alter the pattern of melanin production

Spontaneous Charging and Crystallization of Water Droplets in Oil

We study the spontaneous charging and the crystallization of spherical micron-sized water-droplets dispersed in oil by numerically solving, within a Poisson-Boltzmann theory in the geometry of a spherical cell, for the density profiles of the cations and anions in the system. We take into account screening, ionic Born self-energy differences between oil and water, and partitioning of ions over the two media. We find that the surface charge density of the droplet as induced by the ion partitioning is significantly affected by the droplet curvature and by the finite density of the droplets. We also find that the salt concentration and the dielectric constant regime in which crystallization of the water droplets is predicted is enhanced substantially compared to results based on the planar oil-water interface, thereby improving quantitative agreement with recent experiments.Comment: 10 pages, 7 figures, submitted for publicatio

The TREC2001 video track: information retrieval on digital video information

The development of techniques to support content-based access to archives of digital video information has recently started to receive much attention from the research community. During 2001, the annual TREC activity, which has been benchmarking the performance of information retrieval techniques on a range of media for 10 years, included a ”track“ or activity which allowed investigation into approaches to support searching through a video library. This paper is not intended to provide a comprehensive picture of the different approaches taken by the TREC2001 video track participants but instead we give an overview of the TREC video search task and a thumbnail sketch of the approaches taken by different groups. The reason for writing this paper is to highlight the message from the TREC video track that there are now a variety of approaches available for searching and browsing through digital video archives, that these approaches do work, are scalable to larger archives and can yield useful retrieval performance for users. This has important implications in making digital libraries of video information attainable

Sadržaj

Sadržaj broja 74. časopisa "Dijete, vrtić, obitelj

Sadržaj

Sadržaj broja 74. časopisa "Dijete, vrtić, obitelj

Vrtići kao primjeri održivosti

Mogu li sveučilišta uciti od vrtića?! Na tako postavljeno pitanje, odgovor daje autor Wals u svom članku Zrcaljenje, Gestaltswitching i transformativno socijalno učenje – Početni koraci za razvoj kompetencija održivosti

Migratiepatronen in dynamisch perspectief Een pilotstudie naar veranderende patronen van arbeidsmigratie uit Polen, Bulgarije en Roemenië.

__Abstract__ Sinds de toetreding in 2004 en 2007 van nieuwe EU-lidstaten zijn er naar schatting 340.000 Middenen Oost-Europeanen in Nederland komen wonen en werken (Van der Heijden et al. 2013). De meeste van hen zijn Polen, Roemenen en Bulgaren. Dit aantal overstijgt de aanvankelijke verwachtingen van de Nederlandse overheid (Tijdelijke Commissie, 2011, p. 41) en gaat gepaard met een toegenomen publieke en politieke aandacht voor de thematiek van arbeidsmigranten uit Midden- en Oost- Europa. Een centraal thema daarin is de aard van de arbeidsmigratie. Is vooral sprake van tijdelijke migratie of juist van vestigingsmigratie

Adaptive colour change and background choice behaviour in peppered moth caterpillars is mediated by extraocular photoreception.

Light sensing by tissues distinct from the eye occurs in diverse animal groups, enabling circadian control and phototactic behaviour. Extraocular photoreceptors may also facilitate rapid colour change in cephalopods and lizards, but little is known about the sensory system that mediates slow colour change in arthropods. We previously reported that slow colour change in twig-mimicking caterpillars of the peppered moth (Biston betularia) is a response to achromatic and chromatic visual cues. Here we show that the perception of these cues, and the resulting phenotypic responses, does not require ocular vision. Caterpillars with completely obscured ocelli remained capable of enhancing their crypsis by changing colour and choosing to rest on colour-matching twigs. A suite of visual genes, expressed across the larval integument, likely plays a key role in the mechanism. To our knowledge, this is the first evidence that extraocular colour sensing can mediate pigment-based colour change and behaviour in an arthropod

Predicting Quiescence: The Dependence of Specific Star Formation Rate on Galaxy Size and Central Density at 0.5<z<2.5

In this paper, we investigate the relationship between star formation and structure, using a mass-complete sample of 27,893 galaxies at $0.5<z<2.5$ selected from 3D-HST. We confirm that star-forming galaxies are larger than quiescent galaxies at fixed stellar mass (M$_{\star}$). However, in contrast with some simulations, there is only a weak relation between star formation rate (SFR) and size within the star-forming population: when dividing into quartiles based on residual offsets in SFR, we find that the sizes of star-forming galaxies in the lowest quartile are 0.27$\pm$0.06 dex smaller than the highest quartile. We show that 50% of star formation in galaxies at fixed M$_{\star}$ takes place within a narrow range of sizes (0.26 dex). Taken together, these results suggest that there is an abrupt cessation of star formation after galaxies attain particular structural properties. Confirming earlier results, we find that central stellar density within a 1 kpc fixed physical radius is the key parameter connecting galaxy morphology and star formation histories: galaxies with high central densities are red and have increasingly lower SFR/M$_{\star}$, whereas galaxies with low central densities are blue and have a roughly constant (higher) SFR/M$_{\star}$ at a given redshift. We find remarkably little scatter in the average trends and a strong evolution of $>$0.5 dex in the central density threshold correlated with quiescence from $z\sim0.7-2.0$. Neither a compact size nor high-$n$ are sufficient to assess the likelihood of quiescence for the average galaxy; rather, the combination of these two parameters together with M$_{\star}$ results in a unique quenching threshold in central density/velocity.Comment: 20 pages, 15 figures, and 2 tables; Accepted for publication in the Astrophysical Journa