Gcn5 histone acetyltransferase is present in the mitoplasts

In Saccharomyces cerevisiae the Lysine-acetyltransferase Gcn5 (KAT2) is part of the SAGA complex and is responsible for histone acetylation widely or at specific lysines. In this paper we report that GCN5 deletion differently affects the growth of two strains. The defective mitochondrial phenotype is related to a marked decrease in mtDNA content, which also involves the deletion of specific regions of the molecule. We also show that in wild-type mitochondria the Gcn5 protein is present in the mitoplasts, suggesting a new mitochondrial function independent from the SAGA complex and possibly a new function for this protein connecting epigenetics and metabolism

Virtual Reality for training the public towards unexpected emergency situations

Nowadays, unexpected situations in public spaces are quite frequent; for this reason, there is the need to provide valid decision-making tools to support people’s behavior in emergency situations. The aim of these support tools is to provide a “training” for the public on how to behave when something unexpected happens, in order to make them aware of how to manage and control their own emotions. Thanks to the introduction of new technologies, trainings are also feasible in Virtual Reality (VR), exploiting the chance to create virtual environments and situations that reflect real ones and test different scenarios on a sample of people in order to verify and validate training procedures. Virtual simulations in this context are paramount, because they offer the possibility to analyse reactions and behaviors in a safe, “not real”, so without health concern, environment. Three scenarios (fire, heart attack of a person in the environment and terrorist attack) have been reproduced in VR, analyzing how to define the context for emergency situations. Users approaching the training only know they are going to face a situation without having details on what is happening; this is fundamental to test the training efficiency on people’s reaction

IL POTERE DELLA PAROLA

Di linguaggio è fatta la nostra immagine del mondo, la nostra coscienza, e dallinguaggio dipende il comportamento sociale. In un'analisi del linguaggio verbale edei segni che considera l'aspetto pragmatico, segni e parole non sono solo mezzo dicomunicazione ma fanno parte della prassi, sono essi stessi prassi. Sta qui il poteredella parola e conoscerlo significa non restarne asserviti. Klaus analizza i nuoviprocessi di comunicazione che si sono imposti a partire dal dopoguerra e l'influenzada essi esercitata sulla vita delle persone. Il filosofo si interessa dello sviluppodell'automazione nei processi di produzione, della diffusione capillare dei media, insintesi della rivoluzione tecnico-scientifica che è l'essenza dell'attuale svilupposociale avvalendosi del contributo della semiotica e della cibernetica. Quest'ultima èconsiderata in relazione al materialismo dialettico, e collegata con la logica, lamatematica, la filosofia del linguaggio, la teoria della conoscenza, lasociolinguistica, la psicolinguistica, la didattica delle lingue, ecc

Role of Scaffold Protein Proline-, Glutamic Acid-, and Leucine-Rich Protein 1 (PELP1) in the Modulation of Adrenocortical Cancer Cell Growth

PELP1 acts as an estrogen receptor (ER) coactivator that exerts an essential role in the ER's functions. ER coregulators have a critical role in the progression and response to hormonal treatment of estrogen-dependent tumors. We previously demonstrated that, in adrenocortical carcinoma (ACC), ER\u3b1 is upregulated and that estradiol activates the IGF-II/IGF1R signaling pathways defining the role of this functional cross-talk in H295R ACC cell proliferation. The aim of this study was to determine if PELP1 is expressed in ACC and may play a role in promoting the interaction between ER\u3b1 and IGF1R allowing the activation of pathways important for ACC cell growth. The expression of PELP1 was detected by Western blot analysis in ACC tissues and in H295R cells. H295R cell proliferation decrease was assessed by A3-(4,5-Dimethylthiaoly)-2,5-diphenyltetrazolium bromide (MTT) assay and [3H] thymidine incorporation. PELP1 is expressed in ACC tissues and in H295R cells. Moreover, treatment of H295R with E2 or IGF-II induced a multiprotein complex formation consisting of PELP1, IGF1R, ER\u3b1, and Src that is involved in ERK1/2 rapid activation. PELP1/ER/IGF1R/c-Src complex identification as part of E2- and IGF-II-dependent signaling in ACC suggests PELP1 is a novel and more efficient potential target to reduce ACC growth

The Operative management in Bariatric Acute abdomen (OBA) Survey: long-term complications of bariatric surgery and the emergency surgeon\u27s point of view.

Background: The number of bariatric procedures is increasing worldwide. No consensus or guidelines about the emergency management of long-term complications following bariatric surgery are currently available. The aim of this study is to investigate by a web survey how an emergency surgeon approaches this unique group of patients in an emergency medical scenario and to report their personal experience. Method: An international web survey was sent to 197 emergency surgeons with the aim to collect data about emergency surgeons\u27 experience in the management of patients admitted in the emergency department for acute abdominal pain after bariatric surgery. The survey was conceived as a questionnaire composed by 26 (multiple choice and open) questions and approved by a steering committee. Results: One hundred seventeen international emergency surgeons decided to join the project and answered to the web survey with a response rate of 59.39%. Conclusions: The aim of this WSES web survey was to highlight the current management of patients previously submitted to bariatric surgical procedures by ES.Emergency surgeons must be mindful of postoperative bariatric surgery complications. CT scan with oral intestinal opacification may be useful in making a diagnosis if carefully interpreted by the radiologist and the surgeon.In case of inconclusive clinical and radiological findings, when symptoms fail to improve, surgical exploration for bariatric patients presenting acute abdominal pain, by laparoscopy if expertise is available, is mandatory in the first 12-24 h, to have good outcomes and decrease morbidity rate

Relation between climatic condition, temperature and moisture in the subsoil of Turin Plain

Unconfined aquifer, unsaturated zone, satured zone, Po River, temperatur

Aortic stenting in the growing sheep causes aortic endothelial dysfunction but not hypertension: Clinical implications for coarctation repair

Stent implantation is the treatment of choice for adolescents and adults with aortic coarctation (CoAo). Despite excellent short-term results, 20%-40% of the patients develop arterial hypertension later in life, which was attributed to inappropriate response of the aortic baroreceptors to increased stiffness of the ascending aorta (ASAO), either congenital or induced by CoAo repair. In particular, it has been hypothesized that stent itself may cause or sustain hypertension. Therefore, we aimed to study the hemodynamic and structural impact following stent implantation in the normal aorta of a growing animal

Copper monitoring in vineyard soils of central Italy subjected to three antifungal treatments, and effects of copper sub-lethal doses on the earthworm Eisenia fetida.

none6The extensive employment of copper-based fungicides has increased copper concentration in vineyard soils. The present study’s objectives were to monitor copper concentration in two vineyard soils during two cropping seasons and study the ecotoxicological effects on the earthworm Eisenia fetida. Total, soluble, and bioavailable copper fractions were measured at the end of two cropping seasons and different depths in two vineyards of central Italy, characterised by three anticryptogamic control methods: copper compounds, chitosan, and combined treatments of them. A laboratory experiment to assess the effects on Eisenia fetida was conducted with soil samples collected in the vineyards with a mean copper concentration of 60 mg/kg and two higher concentrations of 90 and 150 mg/kg. Results showed low levels of total copper concentration in the first 20 cm of soils, regardless of antifungal treatment, highlighting prudent management of the vineyards under study, but the soluble fractions showed a significant increase in all samples during the two cropping seasons. At the dose of 150 mg/kg, earthworms suffer during the first two days, showing weight loss and DNA damage, but they are able to recover until day 28, showing no permanent harm at this copper concentration in soil.openArianna De Bernardi, Enrica Marini, Cristiano Casucci, Luca Tiano, Fabio Marcheggiani, Costantino VischettiDE BERNARDI, Arianna; Marini, Enrica; Casucci, Cristiano; Tiano, Luca; Marcheggiani, Fabio; Vischetti, Costantin

Urban groundwater warming in Turin area (NW Italy)

Low enthalpy, geothermal systems, alluvial, temperatur

Italian health professionals on the mandatory COVID-19 vaccine: An online cross-sectional survey

Italy was the first country in Europe to make vaccination against COVID-19 mandatory for healthcare professionals by imposing restrictions in cases of non-compliance. This study investigates the opinions of the Italian healthcare professionals' categories affected by the regulation. We performed a qualitative online survey: the questionnaire comprised both close- and open-ended questions. The final dataset included n = 4,677 valid responses. Responses to closed-ended questions were analyzed with descriptive statistics. The framework method was applied for analyzing the open-ended questions. The sample spanned all health professions subject to compulsory vaccination, with a prevalence of physicians (43.8%) and nurses (26.3%). The vaccine adhesion before the introduction of the obligation was substantial. 10.4% declared not to have adhered to the vaccination proposal. Thirty-five percent of HPs who opted not to get vaccinated said they experienced consequences related to their choice. The trust in the vaccine seems slightly cracked, demonstrating overall vaccine confidence among professionals. Nonetheless, our results show that whether (or not) professionals adhere to vaccination is not a reliable indicator of consent to how it was achieved. There are criticisms about the lawfulness of the obligation. The data show a great variety of participants interpreting their roles concerning public and individual ethics. The scientific evidence motivates ethics-related decisions-the epidemic of confusing and incorrect information affected professionals. The Law triggered an increased disaffection with the health system and conflicts between professionals. Dealing with the working climate should be a commitment to assume soon