Enzyme activities in brown forest soils after introduction of Bacillus thuringiensis-based bioinsecticides

Much attention in the complex of forest pest control methods nowadays is devoted to the application of biological preparations, especially to bacterial formulations produced on the base of Bacillus thuringiensis (BT) that in addition to their high biological effectiveness against injurious insects are safe for man, homoiоtherms, beneficial insects and fish. As is known only 20-40% of sprayed preparation influences directly on pests while its 60-80% by different ways eventually penetrates into the soil. Taking into account also the fact that usage norm of commercial bacterial preparations makes up to 1-3 kg ha^-1^ and that preparation powder contains 45-100 billion viable spores g^-1^ it becomes evident that as a result of spraying huge quantity of bacterial stimulants introduces into the forest soils. In this connection a goal was set to determine the impact of some separately applied domestic insecticides of BT species (BT кб-1, BT кб-2, BT(SAR)-49, BT(SAR)-54, BT(SAR)-86, BT subsp. thuringiensis) introduced into the brown forest soils after spraying on soil enzymatic activity (invertase, urease) defining its fertility. Studies were conducted in 2010 under laboratory conditions. The results obtained indicate that in soils sprayed and non-sprayed by bioinsecticides the activities of invertase and urease undergo to changes from May to August. Maximal activities in soils were registered in June (25.641mg C~6~H~12~O~6~ g^-1^ for invertase and 12.254 mg NH~3~ g^-1^ for urease) and minimal – in May (20.643 mg C~6~H~12~O~6~ g^-1^ for invertase) and in August (9.297 mg NH~3~ g^-1^ for urease) at the average for all variants. By statistical analysis of study results it has been established that there aren’t any significant differences between indices of enzyme activities in sprayed and non-sprayed by biopesticides soils. Study results have led us to the assumption that tested insecticides don’t influence adversely on enzyme activities of brown forest soils and can be widely used in the field of plant protection