22 research outputs found
Glucose Metabolism of Embryos and Endosperms from Deteriorating Barley and Wheat Seeds
A field-grown transgenic tomato line expressing higher levels of polyamines reveals legume cover crop mulch-specific perturbations in fruit phenotype at the levels of metabolite profiles, gene expression, and agronomic characteristics
Genetic modification of crop plants to introduce desirable traits such as nutritional enhancement, disease and pest resistance, and enhanced crop productivity is increasingly seen as a promising technology for sustainable agriculture and boosting food production in the world. Independently, cultural practices that utilize alternative agriculture strategies including organic cultivation subscribe to sustainable agriculture by limiting chemical usage and reduced tillage. How the two together affect fruit metabolism or plant growth in the field or whether they are compatible has not yet been tested. Fruit-specific yeast S-adenosylmethionine decarboxylase (ySAMdc) line 579HO, and a control line 556AZ were grown in leguminous hairy vetch (Vicia villosa Roth) (HV) mulch and conventional black polyethylene (BP) mulch, and their fruit analysed. Significant genotype×mulch-dependent interactions on fruit phenotype were exemplified by differential profiles of 20 fruit metabolites such as amino acids, sugars, and organic acids. Expression patterns of the ySAMdc transgene, and tomato SAMdc, E8, PEPC, and ICDHc genes were compared between the two lines as a function of growth on either BP or HV mulch. HV mulch significantly stimulated the accumulation of asparagine, glutamate, glutamine, choline, and citrate concomitant with a decrease in glucose in the 556AZ fruits during ripening as compared to BP. It enables a metabolic system in tomato somewhat akin to the one in higher polyamine-accumulating transgenic fruit that have higher phytonutrient content. Finally, synergism was found between HV mulch and transgenic tomato in up-regulating N:C indicator genes PEPC and ICDHc in the fruit
Regulation by Auxin of Carbohydrate Metabolism Involved in Cell Wall Synthesis by Pea Stem Tissue
Sweet corn production and efficiency of nitrogen use in high cover crop residue
In the humid, temperate mid-Atlantic area of the USA,
crop production that leaves the soil uncovered can lead to undesirable soil
and nutrient losses to the surrounding Chesapeake Bay watershed. To cope
with this issue, winter annual cover crops could provide soil cover both
during winter months and, as surface residue in no-tillage cropping systems,
during summer months. Legume cover crops such as hairy vetch can produce
abundant biomass and N by the time summer crops are planted in spring.
Although N mineralized from a legume cover crop can contribute to meeting
the N requirement of crops such as corn, it also may not be used efficiently
by crops and could be lost into the local environment. This research was
conducted to determine whether hairy vetch or a hairy vetch-rye mixture that
was allowed to produce high levels of biomass with a high N content (200 to
250 kg/ha) could meet the N requirements of no-tillage sweet corn and to
determine the efficiency of N use relative to that of fertilizer N. Our
results show that marketable yield of sweet corn was approximately doubled
by hairy vetch in 2 of 3 years compared to an unfertilized, no-cover crop
control. However, in 2 of 3 years, hairy vetch and the vetch-rye mix reduced
yield by 19 and 34%, respectively, compared to a no-cover crop control
with fertilizer N. Reduced plant population that reduced the number of ears
per ha accounted for the yield reduction by these cover crops compared to
the fertilized no-cover crop control. Fertilizer N was 1.5 to 2 times more
efficient than hairy vetch at producing sweet corn ear mass per unit of N
input but combinations of fertilizer N with cover crops were less efficient
than either alone. Results suggest that growing sweet corn without tillage
in high biomass levels of cover crops can interfere with crop establishment,
reduce the efficiency of crop production, and allow for potentially high N
losses into the environment