22 research outputs found
Mutation of Tyrosine Residues Involved in the Alkylation Half Reaction of Epoxide Hydrolase from Agrobacterium radiobacter
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Biocatalytic preparation of chiral epichlorohydrins using recombinant Pichia pastoris expressing epoxide hydrolase of Rhodotorula glutinis
No full textThe use of enantioselective hydrolysis for preparing chiral epichlorohydrins was investigated using recombinant Pichia pastoris with the enantioselective epoxide hydrolase of Rhodotorula glutinis. The rate of the recombinant epoxide hydrolase-catalyzed enantioselective hydrolysis of racemic epichlorohydrins was enhanced by the addition of 5% (v/v) Tween 20. Enantiopure (R)-epichlorohydrins with an enantiopurity of 100% ee and a yield of 26% were obtained within 5 min from 50 mM racemates
One-pot biotransformation of racemic styrene oxide into (R)-1,2-phenylethandiol by two recombinant microbial epoxide hydrolases
No full textFormation of Enantiopure 5-Substituted Oxazolidinones through Enzyme-Catalysed Kinetic Resolution of Epoxides
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Purification and characterization of a highly enantioselective epoxide hydrolase from Aspergillus niger
No full textIn vitro evolution of styrene monooxygenase from Pseudomonas putida CA-3 for improved epoxide synthesis
No full textEnantioselective hydrolysis of racemic and meso -epoxides with recombinant escherichia coli expressing epoxide hydrolase from Sphingomonas sp. HXN-200: Preparation of epoxides and vicinal diols in high ee and high concentration
No full text10.1021/cs300804vACS Catalysis34752-75
Active Site of Epoxide Hydrolases Revisited: A Noncanonical Residue in Potato StEH1 Promotes both Formation and Breakdown of the Alkylenzyme Intermediate
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