817 research outputs found
Zur Analyse der anfanglich beim Au-Al-Verbindungsbereich vorkommenden Storungen bein LSI-Bearbeitungsablauf(Materials, Metallurgy & Weldability)
Development of High Power Electron Beam Gun (Report I) : Generation of High Power Electron Beam with LaB_6 Cathode(Physics, Process, Instruments & Measurements)
Untersuchungen der SchweiBguteigenschaften in Abhängigkeiten vom Basizitätsgrad der 50kg/㎟ Grad agglomerierten UP-Schweißpulver(Materials, Metallurgy & Weldability)
Research on Characteristics of Filler Metal and Flux Influencing on the Toughness of a Carbon Steel Weld : Minor Elements and Alloying Elements Influencing on the Toughness of Weld(Materials, Metallurgy & Weldability)
Surface deoxidation mechanism during vacuum heat treatment of stainless steels AISI 304 and 446
A process of removal of surface oxides from stainless steels AISI 304 and 446 that involves reduction by residual carbon, followed by the formation and desorption of carbon monoxide, is studied by investigation of thermal desorption with quadrupole mass spectrometry (QMS) and secondary ion mass spectrometry (SIMS). Carbon monoxide desorption is studied as a function of time and temperature by QMS, and carbon diffusion due to heat treatment is studied with SIMS—twice as much carbon monoxide desorbs from AISI 304 overall and desorption sets in at 900 °C as opposed to 1100 °C for AISI 446. In samples heated to 900 °C, carbon shows surface enrichment in AISI 304 but depletion in AISI 446
Inter-Quark Potentials in Baryons and Multi-Quark Systems in QCD
We perform the first studies of various inter-quark potentials in SU(3) lattice QCD. From the accurate lattice calculation for more than 300
different patterns of three-quark (3Q) systems, we find that the static 3Q
potential is well described by Y-Ansatz, i.e., the Coulomb plus
Y-type linear potential. Quark confinement mechanism in baryons is also
investigated in maximally-Abelian projected QCD. We next study the multi-quark
potentials (=4,5) in SU(3) lattice QCD, and find
that they are well described by the one-gluon-exchange Coulomb plus multi-Y
type linear potential, which supports the flux-tube picture even for the
multi-quarks. Finally, we study the heavy-heavy-light quark (QQq) potential
both in lattice QCD and in a lattice-QCD-based quark model.Comment: Invited talk at the international Conference on Chiral Symmetry in
Hadron and Nuclear Physics (Chiral07) November 13-16, 2007, Osaka University,
Japa
Maintenance of glucose-sensitive insulin secretion of cryopreserved human islets with University of Wisconsin solution and ascorbic acid-2 glucoside
Normal human islet cells are an ideal source for pancreas-targeted cell therapies, but the availability of human donor pancreata for islet isolation is severely limited. To effectively utilize such scarce donor organs for cell therapies, it is crucial to develop an excellent isolation, effective cryopreservation, and efficient gene transfer techniques for the transportation of isolated cells. In the present study, we investigate the effect of University of Wisconsin (UW) solution and ascorbic acid-2 glucoside (AA2G) on the cryopreservation of human islets. We also evaluate the gene transfer efficiency of a lentiviral vector expressing the E. coli LacZ gene, Lt-NLS/LacZ, in human islets. Human islets were isolated with a standard digestion method at the University of Alberta. Isolated islets were transported to Japan for 40 h and then subjected to cryopreservation experiments. The following preservation solutions were tested: UW solution with 100 mug/mL of AA2G, UW solution, 100% fetal bovine serum (FBS), and CMRL supplemented with 10% FBS. Following three months of cryopreservation, the islets were thawed and analyzed for viability, glucose-sensitive insulin secretion, proinsulin gene expression profile, and in vivo engraftment. The islets were also subjected to monolayer formation with 804G-cell-line-derived extracellular matrix (ECM), followed by Lt-NLS/LacZ transduction. The viability, morphology, glucose-sensitive insulin secretion, proinsulin gene expression, and monolayer formation efficiency of the thawed cryopreserved islets are significantly better maintained by the use of UW solution. When AA2G (100 mug/mL) is combined with UW, such parameters are further improved. The adequate engraftment of UW + AA2G-cryopreserved human islets is achieved in the liver of nude mice. Efficient Lt-NLS/LacZ transduction is identified in monolayered islets cryopreserved with UW solution with AA2G. The present work demonstrates that the combination of UW solution with AA2G (100 mug/mL) would be a useful cryopreservation means for human islets. Human islets monolayer-cultured with 804G-derived ECM are efficiently transduced with a lentiviral vector Lt-NLS/LacZ
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