EFFECTS OF PRIMING WITH OR WITHOUTH DRYING ON Pterogyne nitens TUL. SEEDS UNDER STRESS

Pterogyne nitens Tul. \ue9 conhecida popularmente como amendoim do campo,\ue9 uma esp\ue9cie arb\uf3rea, heli\uf3fita, secund\ue1ria inicial que se regenera intensamente em \ue1reas abertas e pastagens. Pode ser empregada como esp\ue9cie ornamental e na reposi\ue7\ue3o de mata ciliar, em l\ub0Cais sujeitos a inunda\ue7\uf5es peri\uf3dicas, em s\uedtios arenosos e degradados. O condicionamento \ue9 uma t\ue9cnica p\uf3s-colheita usada com o objetivo de aumentar a vel\ub0Cidade de germina\ue7\ue3o, emerg\ueancia, bem como ampliar a toler\ue2ncia a v\ue1rios tipos de estresse. O objetivo deste trabalho foi avaliar a efici\ueancia do condicionamento com ou sem secagem posterior em aumentar a resist\ueancia a diferentes tipos de estresse. As sementes selecionadas foram escarificadas com \ue1cido sulf\ufarico durante 15 min. e depois condicionadas em \ue1gua destilada e solu\ue7\uf5es de manitol -0,5 e -1,0 MPa durante 24h a 10\ub0C. Para cada solu\ue7\ue3o de condicionamento, o lote de sementes foi dividido em dois grupos, um dos quais foi seco at\ue9 atingir o teor de umidade apresentado antes do condicionamento, e o segundo foi imediatamente usado nos testes. Os diferentes grupos de sementes foram expostos ao envelhecimento acelerado (100% U.R. sob 35 e 40\ub0C), ao estresse t\ue9rmico (24h a 60 e 70\ub0C) e o teste de exaust\ue3o (24h submersos a 10 e 27\ub0C). Para todos os testes foram utilizadas quatro repeti\ue7\uf5es de 25 unidades e os dados de porcentagem e vel\ub0Cidade de germina\ue7\ue3o foram submetidos \ue0 an\ue1lise de vari\ue2ncia e ao teste de Tukey. Com o aumento da intensidade do estresse houve diminui\ue7\ue3o na germina\ue7\ue3o e no vigor das sementes, que as diferentes formas de condicionamento n\ue3o reverteram. Em geral, o condicionamento com o uso manitol a -1,0 MPa diminuiu a qualidade fisiol\uf3gica das sementes e, solu\ue7\uf5es a -0,5 MPa ou \ue1gua destilada aumentaram a porcentagem e/ou vel\ub0Cidade de germina\ue7\ue3o de sementes submetidas aos diferentes tipos de estresse.Pterogyne nitens Tul. is a arboreal species, with highlight requirements, growing in gaps, grass land and sandy soils . It could be used in streets, to recovery degradated areas with periodical floods. Priming is a post harvest technique, used to improve germination rate, seedling field emergence and stress tolerance. The aim of this work is to contrast, under stress conditions, different ways to the priming. The seeds with 12% of moisture content were selected, scarified with sulfuric acid during 15 min. Distilled water and mannitol solutions (-0.5 MPa and -1.0 MPa) were used to seeds pre imbibition, during 24h at 10\ub0C. A half part of the premed seeds was drying until reach the initial moisture content, and the other part is immediately used in the tests. The accelerated aging was carried out at 100% of R.U. at 35 and 40\ub0C; thermal stress at 60 and 70\ub0C during 24h, and in last test, the seeds were immersed in distilled water at 10 on 27\ub0C. All the tests were conducted with fours replicates of 20 seeds and the data registered were submitted to variance analysis and Tukey test. Seed viability and vigor decreased when stress intensity increased. The priming did not reversed this effect, but in general, mannitol solutions (-1.0 MPa) decreased the seed vigor. The primed seeds in distilled water or mannitol (-0.5 MPa) present an increase in the rate and germination percentage under stress condition

Fl\uf3rula do Parque Nacional da Restinga de Jurubatiba, Rio de Janeiro, Brasil: Amaranthaceae

Volume: 68Start Page: 167End Page: 17

Fl\uf3rula do Parque Nacional da Restinga de Jurubatiba, Rio de Janeiro, Brasil: Asclepiadoideae (Apocynaceae)

Volume: 68Start Page: 177End Page: 18

First record of larvae of Hysterothylacium (Nematoda: Anisakidae) with zoonotic potential in the pirarucu Arapaima gigas (Osteichthyes: Arapaimidae) from South America

Abstract Third-stage larvae (L3) of Hysterothylacium sp. were collected by the first time in juveniles of pirarucu Arapaima gigas farmed in the Rio Preto da Eva, Amazonas state. Ninety-eight (98) out of 100 examined fish showed to be parasitized. Five hundred and ninety larvae of Hysterothylacium sp. were collected from the intestines, stomach and pyloric caeca. The mean intensity of parasite indexes was 6.02 (±5.75) ranging from 1 to 40 larvae per host and the mean abundance was 5.9 (±5.76). The A. gigas is the new host record for larvae of Hysterothylacium sp. in Brazil, and this is the first record of larvae of Hysterothylacium (Nematoda: Anisakidae) with zoonotic potential in the pirarucu from South America

Anakinra and canakinumab for patients with R92Q-associated autoinflammatory syndrome: a multicenter observational study from the AIDA Network

Background: This study aims at describing the therapeutic outcome of patients carrying the R92Q variant in the TNFRSF1A gene treated with anakinra (ANA) or canakinumab (CAN) and identifying any factors predictive of complete response to IL-1 inhibition.Methods: Clinical data of patients treated with ANA or CAN for recurrent inflammatory attacks due to the presence of the R92Q variant were retrospectively collected and analysed.Results: Data about 20 treatment courses with IL-1 inhibitors (16 with ANA and 4 with CAN) from 19 patients were collected. Mean age at disease onset was 20.2 \ub1 14.8 years. In 5 cases (26%) the R92Q variant was found in a family member affected by recurrent fever. The therapeutic response was complete in 13(68%) and partial in 2 patients (11%); treatment failure was observed in 4 cases (21%). Median AIDAI decreased from 10 (interquartile range [IQR] = 28) to 0 (IQR = 1) at the 12-month follow-up visit (p< 0.001). Mean ESR and median CRP dropped respectively from 40.8 \ub1 24.8 to 9.1 \ub1 4.5 mm/h (p< 0.001) and from 3.0 (IQR = 1.9) to 0.3 (IQR = 0.3) mg/dl (p< 0.001) after 12 months of treatment. A steroid-sparing effect was observed from the third month of treatment (p< 0.01). Thirteen patients (65%) were still on treatment at the last follow-up visit (median duration of treatment 17 (IQR = 38) months). The presence of R92Q mutation in a symptomatic relative (p= 0.022), the relapsing remitting disease course (p< 0.001) and the presence of migratory erythematous skin rashes during fever attacks (p= 0.005) were associated with complete efficacy of IL-1 inhibitors.Conclusions: R92Q patients showed a favourable response to ANA and CAN, particularly when the mutation segregated in a family member and when a relapsing-remitting disease course or TNF-\u3b1 receptor-associated periodic syndrome (TRAPS) typical skin rash were observed. In the subgroup of patients not taking advantage of IL-1 blockage different molecular mechanisms underlying the autoinflammatory picture are likely to exist