PHARMACOLOGICAL ACTION OF THE ACTIVE FRACTION-STEREOSPERMUM TETRAGONUM DC

Objective: The objective of the present study was to evaluate the mechanism of action of the active fraction of Stereospermum tetragonum.Methods: An active fraction was isolated from the water extract (active extract by GTT) using ethanol precipitation. To get insight to the mechanism of action of the active fraction, glucose uptake by isolated rat diaphragm was performed. Further the effect of active fraction on glucose tolerance in fed rats was compared with that of fasted rats. Besides, effect of oral administration of the active fraction was compared with that of intra peritonial administration. The effect of active fraction on serum glucose levels in orally glucose loaded rats was compared with that of intra peritoneal glucose loaded rats.Results: Glucose uptake in the isolated rat hemi-diaphragm, were increased by the active fraction at 40 µg/ml concentrations, the active fraction showed promising anti hyper glycemic activity in fed rats when compared with fasted rats. The active fraction was found to be effective in orally glucose loaded in contrast to intra peritonial route. The result shows that glucose absorbtion from an intestinal tract is inhibited by the active fraction.Conclusion: Our findings suggest that this plant is promising for further studies leading to the development of valuable medicine for diabetes.Â