Molecular Weight Dependent Glucose Lowering Effect of Low Molecular Weight Chitosan Oligosaccharide (GO2KA1) on Postprandial Blood Glucose Level in SD Rats Model

Abstract This research investigated the effect of enzymatically digested low molecular weight (MW) chitosan oligosaccharide on type 2 diabetes prevention. Three different chitosan oligosaccharide samples with varying MW were evaluated in vitro for inhibition of rat small intestinal α-glucosidase and porcine pancreatic α-amylase (GO2KA1; \u3c1000 Da, GO2KA2; 1000–10,000 Da, GO2KA3; MW \u3e 10,000 Da). The in vitro results showed that all tested samples had similar rat α-glucosidase inhibitory and porcine α-amylase inhibitory activity. Based on these observations, we decided to further investigate the effect of all three samples at a dose of 0.1 g/kg, on reducing postprandial blood glucose levels in Sprague-Dawley (SD) rat model after sucrose loading test. In the animal trial, all tested samples had postprandial blood glucose reduction effect, when compared to control, however GO2KA1 supplementation had the strongest effect. The glucose peak (Cmax) for GO2KA1 and control was 152 mg/dL and 193 mg/dL, respectively. The area under the blood glucose-time curve (AUC) for GO2KA1 and control was 262 h mg/dL and 305 h mg/dL, respectively. Furthermore, the time of peak plasma concentration of blood glucose (Tmax) for GO2KA1 was significantly delayed (0.9 h) compared to control (0.5 h). These results suggest that GO2KA1 could have a beneficial effect for blood glucose management relevant to diabetes prevention in normal and pre-diabetic individuals. The suggested mechanism of action is via inhibition of the carbohydrate hydrolysis enzyme α-glucosidase and since GO2KA1 (MW \u3c 1000 Da) had higher in vivo effect, we hypothesize that it is more readily absorbed and might exert further biological effect once it is absorbed in the blood stream, relevant to blood glucose management

Anti-Obesity and Anti-Adipogenic Effects of Chitosan Oligosaccharide (GO2KA1) in SD Rats and in 3T3-L1 Preadipocytes Models

Excess body weight is a major risk factor for type 2 diabetes (T2D) and associated metabolic complications, and weight loss has been shown to improve glycemic control and decrease morbidity and mortality in T2D patients. Weight-loss strategies using dietary interventions produce a significant decrease in diabetes-related metabolic disturbance. We have previously reported that the supplementation of low molecular chitosan oligosaccharide (GO2KA1) significantly inhibited blood glucose levels in both animals and humans. However, the effect of GO2KA1 on obesity still remains unclear. The aim of the study was to evaluate the anti-obesity effect of GO2KA1 on lipid accumulation and adipogenic gene expression using 3T3-L1 adipocytes in vitro and plasma lipid profiles using a Sprague-Dawley (SD) rat model. Murine 3T3-L1 preadipocytes were stimulated to differentiate under the adipogenic stimulation in the presence and absence of varying concentrations of GO2KA1. Adipocyte differentiation was confirmed by Oil Red O staining of lipids and the expression of adipogenic gene expression. Compared to control group, the cells treated with GO2KA1 significantly decreased in intracellular lipid accumulation with concomitant decreases in the expression of key transcription factors, peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer-binding protein alpha (CEBP/α). Consistently, the mRNA expression of downstream adipogenic target genes such as fatty acid binding protein 4 (FABP4), fatty acid synthase (FAS), were significantly lower in the GO2KA1-treated group than in the control group. In vivo, male SD rats were fed a high fat diet (HFD) for 6 weeks to induced obesity, followed by oral administration of GO2KA1 at 0.1 g/kg/body weight or vehicle control in HFD. We assessed body weight, food intake, plasma lipids, levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) for liver function, and serum level of adiponectin, a marker for obesity-mediated metabolic syndrome. Compared to control group GO2KA1 significantly suppressed body weight gain (185.8 ± 8.8 g vs. 211.6 ± 20.1 g, p \u3c 0.05) with no significant difference in food intake. The serum total cholesterol, triglyceride, and low-density lipoprotein (LDL) levels were significantly lower in the GO2KA1-treated group than in the control group, whereas the high-density lipoprotein (HDL) level was higher in the GO2KA1 group. The GO2KA1-treated group also showed a significant reduction in ALT and AST levels compared to the control. Moreover, serum adiponectin levels were significantly 1.5-folder higher than the control group. These in vivo and in vitro findings suggest that dietary supplementation of GO2KA1 may prevent diet-induced weight gain and the anti-obesity effect is mediated in part by inhibiting adipogenesis and increasing adiponectin level

Inmate cancer patients – highlighting the importance of a holistic approach to oncological care

Purpose: Inmate oncologic patients' rates increased drastically worldwide. Elderly, limited exercise, unhealthy diet, hepatitis, HIV+ status, tobacco and alcohol use, constitute the main cancer risk factors. We present an outline of practical oncological management and ethical thinking, in the specific environment of a detention facility. Methods: PubMed, Cochrane Database of Controlled Trials, SCOPUS and grey literature were extensively searched upto October 2021. Ιncarcerated oncologic patients experiencevarious everyday challenges:their confinement in high securityfacilities, the lack of access to critical care and related ethicaldilemmas inherent to the context of a correctional facility. Results: The detention facilities may be inadequate in providing early cancer diagnosis and appropriate care mainlydue to a lack of specialized personnel, b) in-house or in external specialized cancer hospitals, care variability (e.g. admissions in small local or regional hospitals), c) delays inproviding access and d) gatekeeper systems. There is a paucity of administration of a) systemic therapy(chemotherapy, targeted drug therapy etc), b) radiotherapy, c)palliative care, and d) enrollment in clinical trials.  Conclusions: Correctional facilities must encourage teamwork between healthcare and correctional professionals inorder to improve the provided anticancer care

Lactic acid fermentation and phytochemical synergies for food safety and human health applications

Type 2 diabetes, hypertension and Helicobacter pylori -linked ulcer are among the important global health challenges. Healthier food and ingredient design for addressing above health challenges could be combined with traditional drug therapies for disease management. Listeria monocytogenes, Staphylococcus aureus, Vibrio parahaemolyticus and Escherichia coli are food-borne pathogens associated with infectious disease outbreaks. However, the development of antimicrobial resistance and the limited success of relatively narrow spectrum of antimicrobials, results in microbial contamination of food and poses public health and economic challenge. Therefore, the aim of this dissertation was to develop metabolic strategies for designing milk and soymilk fermented diets and ingredients enriched in phenolic phytochemicals for managing type 2 diabetes, hypertension, ulcer and controlling food-borne pathogens. This was investigated through understanding the in vitro inhibitory potential of hyperglycemia and hypertension linked enzymes as well as critical metabolic pathways contributing to the cellular breakdown of bacterial pathogens. Based on structure-function rationale, the potential antioxidant, anti-hyperglycemia and anti-hypertention functionality of herb and fruit extracts, fruit enriched dairy and soy yogurts and herb and fruit enriched cheeses were investigated. Results indicated that specific extract profiles had high anti-hyperglycemia and anti-hypertension potential which generally corresponded to total phenolic content and free radical scavenging-linked antioxidant activity. Further it was observed that lactic acid bacterial fermented milk and soymilk have potential for hyperglycemia and hypertension management, which is enhanced with phenolic phytochemical enrichment. However, it was determined that soymilk fermented products had superior potential for hypertension management and further that the anti-hypertension potential of fermented milk depended strongly on the lactic acid produced during fermentation. Furthermore these fermented extracts had potential to inhibit L. monocytogenes, S. aureus , V. parahaemolyticus, and ulcer related H. pylori. Results also indicated that oregano-cranberry synergies with sodium lactate had the best potential for L. monocytogenes inhibition in broth and cooked meat substrate and that this inhibition recovered with the addition of proline. Finally the mode of action of cranberry phenolics and L-lactic acid was investigated in L. monocytogenes and E. coli based on the concept of disruption of critical proline-linked pentose phosphate pathway and the role of this pathway was confirmed

Recovery of Bioactive Peptides and Omega-3 Fatty Acids-Containing Phospholipids from Squid Processing By-Product Hydrolysate

ABSTRACT: The present study examined whether bioactive peptides and omega-3 fatty acids-containing phopholipids could be recovered from squid processing by-product (SPB) hydrolysate. The hydrolysate was produced at 55°C for varying times with endogenous proteases and centrifuged to yield peptides-containing supernatant and phospholipids-containing precipitate. The supernatant showed angiotensin I-converting enzyme (ACE) inhibitory activity with half maximal inhibitory concentration (IC50) values decreasing with hydrolysis time from 2.11 mg (0 h) to 1.71 mg (1 h), 1.38 mg (1.5 h), and 1.34 mg (2 h). Two-hour squid hydrolysate was further fractionated to isolate the most active fraction whose molecular weight was found to be below 10 kDa with IC50 of 0.32 mg. The phospholipids-containing precipitate (45.6 g/100 g oil) was analyzed for a fatty acid profile, with the levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) being 16.86 and 29.24 g/100 g oil, respectively. The ACE inhibition by hydrolysate peptides and omega-3 fatty acid recovery support the nutraceutical potential of the SPB hydrolysate

Comparison of direct steam injection and steam-jacketed heating in squid protein hydrolysis for energy consumption and hydrolysis performance

Direct steam injection (DSI) and conventional steam-jacketed (SJ) heating systems were compared for energy consumption and hydrolysis performance in producing squid hydrolysates (SH). Hydrolysis was carried out with endogenous enzymes at 55°C for 90min and native pH (6.5±0.2), followed by pasteurization at 75°C for 30min. Hydrolysis performance was evaluated by monitoring the changes in viscosity, degree of hydrolysis (DH), and protein profile during the course of hydrolysis. The DSI heating process was more energy efficient than SJ heating with significantly less energy usage (~30% energy reduction), shorter come-up times, and total processing time. While considerable fouling was evident with SJ heating, no fouling was observed with DSI heating. No appreciable differences in hydrolysis performance were observed between DSI and SJ heating, although the DSI-treated hydrolysate exhibited slightly lower values in viscosity and DH, as well as weaker protein band intensities due to dilution caused by steam condensation. The use of a steam filtration unit in DSI not only filtered the incoming steam, but also reduced condensation. Results suggest that DSI heating with steam filtration is an energy efficient and fouling-free process for preparing SH and potentially for other type of enzymatic protein hydrolysates. © 2014 Elsevier Ltd

In vitro evaluation of phenolic-enriched maple syrup extracts for inhibition of carbohydrate hydrolyzing enzymes relevant to type 2 diabetes management

Phenolic-enriched extracts of Canadian maple syrup, namely ethyl acetate (MS-EtOAc) and butanol (MS-BuOH), were evaluated for their ability to inhibit carbohydrate hydrolyzing enzymes relevant to type 2 diabetes management. Extracts were standardized to phenolic contents by the Folin-Ciocalteau method and assayed for yeast α-glucosidase inhibitory activities. On normalization to phenolic content, MS-BuOH exhibited higher inhibitory activity than MS-EtOAc (IC50=68.38 and 107.9μg phenolics, respectively). The extracts were further assayed for inhibition of porcine α-amylase and rat α-glucosidase enzymes. MS-BuOH exhibited higher rat α-glucosidase and porcine α-amylase inhibitory activities (IC50=135 and 103μg phenolics, respectively) than MS-EtOAC extract (IC50\u3e187μg phenolics in both assays). These results suggest that maple syrup extracts may have potential for phenolic-mediated type 2 diabetes management, with the MS-BuOH phenolic-enriched fraction having highest bioactivity. © 2011 Elsevier Ltd

The Effect of Tannin-Rich Witch Hazel on Growth of Probiotic <i>Lactobacillus plantarum</i>

Probiotic bacteria help maintain microbiome homeostasis and promote gut health. Maintaining the competitive advantage of the probiotics over pathogenic bacteria is a challenge, as they are part of the gut microbiome that is continuously exposed to digestive and nutritional changes and various stressors. Witch hazel that is rich in hamamelitannin (WH, whISOBAXTM) is an inhibitor of growth and virulence of pathogenic bacteria. To test for its effect on probiotic bacteria, WH was tested on the growth and biofilm formation of a commercially available probiotic Lactobacillus plantarum PS128. As these bacteria are aerotolerant, the experiments were carried out aerobically and in nutritionally inadequate/poor (nutrient broth) or adequate/rich (MRS broth) conditions. Interestingly, despite its negative effect on the growth and biofilm formation of pathogenic bacteria such as Staphylococcus epidermidis, WH promotes the growth of the probiotic bacteria in a nutritionally inadequate environment while maintaining their growth under a nutritionally rich environment. In the absence of WH, no significant biofilm is formed on the surfaces tested (polystyrene and alginate), but in the presence of WH, biofilm formation was significantly enhanced. These results indicate that WH may thus be used to enhance the growth and survival of probiotics